SCARB1 Antibody | Affinity Biosciences

IHC
IF/ICC
Cites

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DF6479 at 1/100 staining Human prostate cancer by IHC-P.

DF6479 at 1/100 staining Mouse kidney tissue by IHC-P.

DF6479 at 1/100 staining Mouse brain tissue by IHC-P.

DF6479 at 1/100 staining Rat brain tissue by IHC-P.

DF6479 staining HEPG2 cells by IF/ICC.

FIGURE 6| (a) Representative Masson staining images of the heart papillary muscle cross‐sectional scan images, and infarct rate of the different groups.

FIGURE 2 The expression of cholesterol metabolism-related proteins in the cortex post-TBI.

Product:	SCARB1 Antibody
Catalog:	DF6479
Description:	Rabbit polyclonal antibody to SCARB1
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	61kDa; 61kD(Calculated).
Uniprot:	Q8WTV0
RRID:	AB_2838441

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(91%), Horse(91%), Sheep(82%), Rabbit(91%), Dog(91%)

Clonality:

Polyclonal

Specificity:

SCARB1 Antibody detects endogenous levels of total SCARB1.

RRID:

AB_2838441
Cite Format: Affinity Biosciences Cat# DF6479, RRID:AB_2838441.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CD36 and LIMPII analogous 1; CD36; CD36 Antigen like 1; CD36 antigen-like 1; CD36L1; CLA 1; CLA-1; CLA1; Collagen type I receptor; HDLQTL6; MGC138242; SCARB1; Scavebger Receptor Class B Member 1; Scavenger receptor class B member 1; Scavenger Receptor Class B Type 1; SCRB1_HUMAN; SR BI; SR-BI; SRB1; SRBI; Thrombospondin receptor like 1; thrombospondin receptor-like 1;

Immunogens

Immunogen:

Uniprot:

Q8WTV0(SCRB1_HUMAN) >>Visit HPA database.

Gene(ID):

SCARB1(949)

Expression:

Q8WTV0 SCRB1_HUMAN:

Widely expressed.

Description:

The protein encoded by this gene is a plasma membrane receptor for high density lipoprotein cholesterol (HDL). The encoded protein mediates cholesterol transfer to and from HDL. In addition, this protein is a receptor for hepatitis C virus glycoprotein E2. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]

Sequence:

Q8WTV0 SCRB1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGCSAKARWAAGALGVAGLLCAVLGAVMIVMVPSLIKQQVLKNVRIDPSSLSFNMWKEIPIPFYLSVYFFDVMNPSEILKGEKPQVRERGPYVYREFRHKSNITFNNNDTVSFLEYRTFQFQPSKSHGSESDYIVMPNILVLGAAVMMENKPMTLKLIMTLAFTTLGERAFMNRTVGEIMWGYKDPLVNLINKYFPGMFPFKDKFGLFAELNNSDSGLFTVFTGVQNISRIHLVDKWNGLSKVDFWHSDQCNMINGTSGQMWPPFMTPESSLEFYSPEACRSMKLMYKESGVFEGIPTYRFVAPKTLFANGSIYPPNEGFCPCLESGIQNVSTCRFSAPLFLSHPHFLNADPVLAEAVTGLHPNQEAHSLFLDIHPVTGIPMNCSVKLQLSLYMKSVAGIGQTGKIEPVVLPLLWFAESGAMEGETLHTFYTQLVLMPKVMHYAQYVLLALGCVLLLVPVICQIRSQVGAGQRAARADSHSLACWGKGASDRTLWPTAAWSPPPAAVLRLCRSGSGHCWGLRSTLASFACRVATTLPVLEGLGPSLGGGTGS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Dog

Rabbit

Sheep

Bovine

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8WTV0 As Substrate

Q8WTV0

Site	PTM Type	Source
K42	Ubiquitination	Uniprot
Y92	Phosphorylation	Uniprot
N102	N-Glycosylation	Uniprot
T175	Phosphorylation	Uniprot
K284	Ubiquitination	Uniprot
K288	Ubiquitination	Uniprot
N330	N-Glycosylation	Uniprot

Research Backgrounds

Q8WTV0_SCRB1_HUMAN

Function:

Receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and apoptotic cells. Receptor for HDL, mediating selective uptake of cholesteryl ether and HDL-dependent cholesterol efflux. Also facilitates the flux of free and esterified cholesterol between the cell surface and apoB-containing lipoproteins and modified lipoproteins, although less efficiently than HDL. May be involved in the phagocytosis of apoptotic cells, via its phosphatidylserine binding activity.

(Microbial infection) Acts as a receptor for hepatitis C virus in hepatocytes and appears to facilitate its cell entry. Binding between SCARB1 and the hepatitis C virus glycoprotein E2 is independent of the genotype of the viral isolate. Mediates uptake of M.fortuitum, E.coli and S.aureus.

PTMs:

N-glycosylated.

The six cysteines of the extracellular domain are all involved in intramolecular disulfide bonds.

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Membrane>Caveola>Multi-pass membrane protein.
Note: Predominantly localized to cholesterol and sphingomyelin-enriched domains within the plasma membrane, called caveolae.

Tissue Specificity:

Widely expressed.

Subunit Structure:

The C-terminal region binds to PDZK1.

(Microbial infection) Interacts with hepatitis C virus E1:E2 glycoproteins.

Family&Domains:

Belongs to the CD36 family.

Research Fields

· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Organismal Systems > Endocrine system > Ovarian steroidogenesis.

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

· Organismal Systems > Digestive system > Fat digestion and absorption.

· Organismal Systems > Digestive system > Vitamin digestion and absorption.

· Organismal Systems > Digestive system > Cholesterol metabolism.

References

1). Brain-specific loss of Abcg1 disturbs cholesterol metabolism and aggravates pyroptosis and neurological deficits after traumatic brain injury. Brain Pathology [IF=6.4]

Application: WB Species: Mouse Sample: brain

FIGURE 2 The expression of cholesterol metabolism-related proteins in the cortex post-TBI. (A) Representative western blotting bands of ABCA1, ABCG1, ABCG4, SCARB1, ApoE, LCAT, RXRalpha, PPARgamma, LXRalpha, and beta-actin in the cortex at the indicated time point post-TBI. (B) The relative optical densitometric quantification of the proteins was normalized against beta-actin. (C, D) A panoramic view of brain slice pointed out the location of injured cortical brain regions used for staining and cell counts. Distribution and semi-quantitative analysis of ABCA1 and ABCG1 in the injured cortex on Day 3 after TBI. Examples of co-localization of ABCA1/ABCG1 with the neuron marker NeuN, the astrocyte marker GFAP, and the microglia marker Iba-1 using white arrows. (E, F) The immunohistochemical staining and semi-quantitative analysis of ABCA1 and ABCG1 in the injured and normal cortex in the human brain. Examples of ABCA1/ABCG1-positive cell using white arrows. Data are mean ± SEM; *p

2). Silica-coated magnetic nanoparticles labeled endothelial progenitor cells alleviate ischemic myocardial injury and improve long-term cardiac function with magnetic field guidance in rats with myocardial infarction. JOURNAL OF CELLULAR PHYSIOLOGY (PubMed: 30982985) [IF=5.6]

Application: WB Species: rat Sample: EPCs

FIGURE 6| (a) Representative Masson staining images of the heart papillary muscle cross‐sectional scan images, and infarct rate of the different groups. (b) Upper panel: Bar graphs of LVEF and LVFS before and at 4 weeks after the transplantation of EPCs in the different groups.Lower panel: Western blots analysis of the expression of ANP and BNP in the different groups.

3). A Novel Rat Model of Patellofemoral Osteoarthritis Due to Patella Baja, or Low-Lying Patella. MEDICAL SCIENCE MONITOR (PubMed: 30979862) [IF=3.1]

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SCARB1 Antibody - #DF6479