NR1I2 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from B16F10, using NR1I2 Antibody.

Product:	NR1I2 Antibody
Catalog:	DF6478
Description:	Rabbit polyclonal antibody to NR1I2
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	50kDa; 50kD(Calculated).
Uniprot:	O75469
RRID:	AB_2838440

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(91%), Bovine(80%), Horse(82%), Sheep(80%), Rabbit(91%), Dog(82%)

Clonality:

Polyclonal

Specificity:

NR1I2 Antibody detects endogenous levels of total NR1I2.

RRID:

AB_2838440
Cite Format: Affinity Biosciences Cat# DF6478, RRID:AB_2838440.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

BXR; Nr1i2; NR1I2_HUMAN; Nuclear receptor subfamily 1 group I member 2; ONR 1; ONR1; Orphan nuclear receptor PAR 1; Orphan nuclear receptor PAR1; Orphan nuclear receptor PXR; OTTHUMP00000215173; OTTHUMP00000215174; OTTHUMP00000215175; PAR 1; PAR 2; PAR; PAR q; PAR1; PAR2; PARq; pregnane X nuclear receptor variant 2; Pregnane X receptor; PRR; PXR; SAR; Steroid and xenobiotic receptor; SXR;

Immunogens

Immunogen:

Uniprot:

O75469(NR1I2_HUMAN) >>Visit HPA database.

Gene(ID):

NR1I2(8856)

Expression:

O75469 NR1I2_HUMAN:

Expressed in liver, colon and small intestine.

Description:

This gene product belongs to the nuclear receptor superfamily, members of which are transcription factors characterized by a ligand-binding domain and a DNA-binding domain. The encoded protein is a transcriptional regulator of the cytochrome P450 gene CYP3A4, binding to the response element of the CYP3A4 promoter as a heterodimer with the 9-cis retinoic acid receptor RXR. It is activated by a range of compounds that induce CYP3A4, including dexamethasone and rifampicin. Several alternatively spliced transcripts encoding different isoforms, some of which use non-AUG (CUG) translation initiation codon, have been described for this gene. Additional transcript variants exist, however, they have not been fully characterized.

Sequence:

O75469 NR1I2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEVRPKESWNHADFVHCEDTESVPGKPSVNADEEVGGPQICRVCGDKATGYHFNVMTCEGCKGFFRRAMKRNARLRCPFRKGACEITRKTRRQCQACRLRKCLESGMKKEMIMSDEAVEERRALIKRKKSERTGTQPLGVQGLTEEQRMMIRELMDAQMKTFDTTFSHFKNFRLPGVLSSGCELPESLQAPSREEAAKWSQVRKDLCSLKVSLQLRGEDGSVWNYKPPADSGGKEIFSLLPHMADMSTYMFKGIISFAKVISYFRDLPIEDQISLLKGAAFELCQLRFNTVFNAETGTWECGRLSYCLEDTAGGFQQLLLEPMLKFHYMLKKLQLHEEEYVLMQAISLFSPDRPGVLQHRVVDQLQEQFAITLKSYIECNRPQPAHRFLFLKIMAMLTELRSINAQHTQRLLRIQDIHPFATPLMQELFGITGS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

Pig

Horse

Dog

Bovine

Sheep

Zebrafish

Xenopus

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75469 As Substrate

O75469

Site	PTM Type	Enzyme	Source
S8	Phosphorylation		Uniprot
T57	Phosphorylation		Uniprot
K101	Ubiquitination		Uniprot
K109	Acetylation		Uniprot
S114	Phosphorylation	P24941 (CDK2)	Uniprot
T133	Phosphorylation	P24941 (CDK2)	Uniprot
T135	Phosphorylation	P24941 (CDK2)	Uniprot
S167	Phosphorylation	P24941 (CDK2)	Uniprot
S200	Phosphorylation	P24941 (CDK2)	Uniprot
S208	Phosphorylation		Uniprot
T248	Phosphorylation		Uniprot
Y249	Phosphorylation		Uniprot
S256	Phosphorylation		Uniprot
S274	Phosphorylation		Uniprot
T290	Phosphorylation		Uniprot
S305	Phosphorylation		Uniprot
S350	Phosphorylation	P24941 (CDK2)	Uniprot
T408	Phosphorylation	P17252 (PRKCA)	Uniprot
T422	Phosphorylation		Uniprot

Research Backgrounds

O75469_NR1I2_HUMAN

Function:

Nuclear receptor that binds and is activated by variety of endogenous and xenobiotic compounds. Transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, drugs and endogenous compounds. Activated by the antibiotic rifampicin and various plant metabolites, such as hyperforin, guggulipid, colupulone, and isoflavones. Response to specific ligands is species-specific. Activated by naturally occurring steroids, such as pregnenolone and progesterone. Binds to a response element in the promoters of the CYP3A4 and ABCB1/MDR1 genes.

Subcellular Location:

Nucleus.

Tissue Specificity:

Expressed in liver, colon and small intestine.

Subunit Structure:

Heterodimer with RXR. Interacts with NCOA1. Interacts (via domain NR LBD) with CRY1 and CRY2 in a ligand-dependent manner (By similarity).

Family&Domains:

Belongs to the nuclear hormone receptor family. NR1 subfamily.

References

1). Indole-3-propionic Acid Attenuates HI-Related Blood–Brain Barrier Injury in Neonatal Rats by Modulating the PXR Signaling Pathway. ACS Chemical Neuroscience, 2022 (PubMed: 36129348) [IF=5.0]

2). Construction of Humanized CYP1A2 Rats Using CRISPR/CRISPR-Associated Protein 9 to Promote Drug Metabolism and Pharmacokinetic Research. Drug Metabolism and Disposition, 2024 (PubMed: 37884392)

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NR1I2 Antibody - #DF6478