SERPINC1 Antibody | Affinity Biosciences

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Western blot analysis of extracts from HepG2, using SERPINC1 Antibody.

Product:	SERPINC1 Antibody
Catalog:	DF6470
Description:	Rabbit polyclonal antibody to SERPINC1
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	53kDa; 53kD(Calculated).
Uniprot:	P01008
RRID:	AB_2838432

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(91%), Bovine(100%), Horse(100%), Sheep(91%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

SERPINC1 Antibody detects endogenous levels of total SERPINC1.

RRID:

AB_2838432
Cite Format: Affinity Biosciences Cat# DF6470, RRID:AB_2838432.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ANT3_HUMAN; Antithrombin; Antithrombin III; Antithrombin-III; AntithrombinIII; AT 3; AT III; AT3; AT3D; ATIII; Heparin cofactor I; MGC22579; Serine (or cysteine) proteinase inhibitor clade C (antithrombin) member 1; Serine cysteine proteinase inhibitor clade C member 1; Serine proteinase inhibitor clade C member 1; Serpin C1; Serpin family C member 1; Serpin peptidase inhibitor clade C (antithrombin) member 1; SERPINC1; THPH7;

Immunogens

Immunogen:

Uniprot:

P01008(ANT3_HUMAN) >>Visit HPA database.

Gene(ID):

SERPINC1(462)

Expression:

P01008 ANT3_HUMAN:

Found in plasma.

Description:

SERPINC1, also named Antithrombin III or Antithrombin, is a plasma protease inhibitor and a member of the serpin superfamily. This protein inhibits thrombin as well as other activated serine proteases of the coagulation system, and it regulates the blood coagulation cascade. The protein includes two functional domains: the heparin binding-domain at the N-terminus of the mature protein, and the reactive site domain at the C-terminus. The inhibitory activity is enhanced by the presence of heparin. More than 120 mutations have been identified for this gene, many of which are known to cause antithrombin-III deficiency.

Sequence:

P01008 ANT3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MYSNVIGTVTSGKRKVYLLSLLLIGFWDCVTCHGSPVDICTAKPRDIPMNPMCIYRSPEKKATEDEGSEQKIPEATNRRVWELSKANSRFATTFYQHLADSKNDNDNIFLSPLSISTAFAMTKLGACNDTLQQLMEVFKFDTISEKTSDQIHFFFAKLNCRLYRKANKSSKLVSANRLFGDKSLTFNETYQDISELVYGAKLQPLDFKENAEQSRAAINKWVSNKTEGRITDVIPSEAINELTVLVLVNTIYFKGLWKSKFSPENTRKELFYKADGESCSASMMYQEGKFRYRRVAEGTQVLELPFKGDDITMVLILPKPEKSLAKVEKELTPEVLQEWLDELEEMMLVVHMPRFRIEDGFSLKEQLQDMGLVDLFSPEKSKLPGIVAEGRDDLYVSDAFHKAFLEVNEEGSEAAASTAVVIAGRSLNPNRVTFKANRPFLVFIREVPLNTIIFMGRVANPCVK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Dog

100

Rabbit

100

Pig

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P01008 As Substrate

P01008

Site	PTM Type	Source
T10	Phosphorylation	Uniprot
S57	Phosphorylation	Uniprot
T63	Phosphorylation	Uniprot
S68	Phosphorylation	Uniprot
N128	N-Glycosylation	Uniprot
S174	Phosphorylation	Uniprot
N187	N-Glycosylation	Uniprot
S214	Phosphorylation	Uniprot
N224	N-Glycosylation	Uniprot
Y272	Phosphorylation	Uniprot

Research Backgrounds

P01008_ANT3_HUMAN

Function:

Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.

PTMs:

Phosphorylated by FAM20C in the extracellular medium.

Subcellular Location:

Secreted>Extracellular space.

Tissue Specificity:

Found in plasma.

Subunit Structure:

Forms protease inhibiting heterodimer with TMPRSS7.

Family&Domains:

Belongs to the serpin family.

Research Fields

· Organismal Systems > Immune system > Complement and coagulation cascades. (View pathway)

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SERPINC1 Antibody - #DF6470