Annexin A2 Antibody - #DF6468

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Product: Annexin A2 Antibody
Catalog: DF6468
Description: Rabbit polyclonal antibody to Annexin A2
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 39kDa; 39kD(Calculated).
Uniprot: P07355
RRID: AB_2838430

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(91%)
Clonality:
Polyclonal
Specificity:
Annexin A2 Antibody detects endogenous levels of total Annexin A2.
RRID:
AB_2838430
Cite Format: Affinity Biosciences Cat# DF6468, RRID:AB_2838430.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Annexin A2; Annexin II; Annexin II, heavy chain; Annexin-2; ANX 2; ANX2; ANX2L4; ANXA2; ANXA2_HUMAN; arylsulfatase B; CAL1H; Calpactin I heavy chain; calpactin I heavy polypeptide (p36); Calpactin I heavy polypeptide; Calpactin-1 heavy chain; chromobindin 8; Chromobindin-8; Epididymis secretory protein Li 270; HEL S 270; LIP2; Lipocortin II; LPC2; LPC2D; p36; P36 protein; PAP-IV; Placental anticoagulant protein IV; Protein I;

Immunogens

Immunogen:
Uniprot:

P07355(ANXA2_HUMAN) >>Visit HPA database.

Gene(ID):
ANXA2(302)
Description:
Annexin A2 (ANXA2), also known as lipocortin II or calpactin-1 heavy chain, is a 36 kDa member of the annexin superfamily that binds phospholipids and other proteins in a calcium-dependent manner via annexin repeats (1). Annexin A2 contains four such repeats through which it mediates protein-protein and protein-lipid interactions (1-4). It forms a constitutive heterotetramer with S100A10, acting as a bridge between the actin cytoskeleton, plasma membrane, and endocytotic vesicle machinery (5-7). Originally identified as a protein inhibitor of phospholipase A2, annexin A2 has subsequently been shown to interact with an array of protein and non-protein partners, including F-actin, spectrin, SNARE complexes, RNA, and virus particles (4,6,8,9). Annexin A2 has also been shown to have receptor-like activity and is detected on the surface of macrophages and vascular endothelial cells where it mediates macrophage activation and Factor Xa signaling, respectively (10-13). Upregulation of annexin A2 at the cell surface is thought to be modulated by phosphorylation at Tyr23 by Src (14-18). Interestingly, phosphorylation at Tyr23 has recently been shown to be required for cell surface expression of annexin A2 where it mediates motility, invasiveness, and overall metastatic potential of certain pancreatic cancer cells (19,20).
Sequence:
MSTVHEILCKLSLEGDHSTPPSAYGSVKAYTNFDAERDALNIETAIKTKGVDEVTIVNILTNRSNAQRQDIAFAYQRRTKKELASALKSALSGHLETVILGLLKTPAQYDASELKASMKGLGTDEDSLIEIICSRTNQELQEINRVYKEMYKTDLEKDIISDTSGDFRKLMVALAKGRRAEDGSVIDYELIDQDARDLYDAGVKRKGTDVPKWISIMTERSVPHLQKVFDRYKSYSPYDMLESIRKEVKGDLENAFLNLVQCIQNKPLYFADRLYDSMKGKGTRDKVLIRIMVSRSEVDMLKIRSEFKRKYGKSLYYYIQQDTKGDYQKALLYLCGGDD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
91
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P07355 As Substrate

Site PTM Type Enzyme
S2 Phosphorylation
T3 Phosphorylation
K10 Acetylation
K10 Ubiquitination
S12 Phosphorylation P17252 (PRKCA)
S18 Phosphorylation
T19 Phosphorylation
S22 Phosphorylation
Y24 Phosphorylation P12931 (SRC)
S26 Phosphorylation P05771 (PRKCB) , P17252 (PRKCA)
K28 Ubiquitination
Y30 Phosphorylation
T31 Phosphorylation
T44 Phosphorylation
K47 Acetylation
K47 Ubiquitination
K49 Acetylation
K49 Sumoylation
K49 Ubiquitination
T55 Phosphorylation
T61 Phosphorylation
Y75 Phosphorylation
K81 Ubiquitination
S85 Phosphorylation
K88 Ubiquitination
S89 Phosphorylation
S92 Phosphorylation
T97 Phosphorylation
K104 Acetylation
K104 Ubiquitination
T105 Phosphorylation
Y109 Phosphorylation
S112 Phosphorylation
K115 Acetylation
K115 Ubiquitination
S117 Phosphorylation
K119 Ubiquitination
T123 Phosphorylation
S127 Phosphorylation
C133 S-Nitrosylation
S134 Phosphorylation
T136 Phosphorylation
Y147 Phosphorylation
K148 Acetylation
K148 Ubiquitination
K152 Acetylation
K152 Ubiquitination
K157 Acetylation
K157 Ubiquitination
S161 Phosphorylation
T163 Phosphorylation
S164 Phosphorylation
K169 Methylation
K169 Ubiquitination
K176 Acetylation
K176 Ubiquitination
S184 Phosphorylation
Y188 Phosphorylation
Y199 Phosphorylation
K204 Acetylation
K204 Ubiquitination
K212 Acetylation
K212 Ubiquitination
S215 Phosphorylation
T218 Phosphorylation
S221 Phosphorylation
K227 Acetylation
K227 Methylation
K227 Ubiquitination
Y232 Phosphorylation
K233 Acetylation
K233 Ubiquitination
S234 Phosphorylation
Y235 Phosphorylation
S236 Phosphorylation
Y238 Phosphorylation
S243 Phosphorylation
K246 Ubiquitination
K249 Ubiquitination
C262 S-Nitrosylation
K266 Sumoylation
K266 Ubiquitination
Y269 Phosphorylation
Y275 Phosphorylation
S277 Phosphorylation
K279 Acetylation
K279 Ubiquitination
K281 Ubiquitination
S296 Phosphorylation
K302 Acetylation
K302 Ubiquitination
S305 Phosphorylation
K310 Acetylation
K313 Acetylation
K313 Methylation
K313 Ubiquitination
S314 Phosphorylation
Y316 Phosphorylation
Y317 Phosphorylation
Y318 Phosphorylation
T323 Phosphorylation
K324 Acetylation
K324 Sumoylation
K324 Ubiquitination
K329 Ubiquitination
Y333 Phosphorylation

Research Backgrounds

Function:

Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. Inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism but also competes with LDLR for binding with PCSK9.

PTMs:

Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface.

ISGylated.

Subcellular Location:

Secreted>Extracellular space>Extracellular matrix>Basement membrane. Melanosome.
Note: In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Heterotetramer containing 2 light chains of S100A10/p11 and 2 heavy chains of ANXA2/p36 (By similarity). Interacts with ATP1B1 (By similarity). Interacts with DYSF (By similarity). Interacts with COCH. Interacts (via repeat Annexin 1) with PCSK9 (via the C-terminal domain); the interaction inhibits the degradation of LDLR. Interacts with CEACAM1 (via the cytoplasmic domain); this interaction is regulated by phosphorylation of CEACAM1. Interacts with APPL2 and APPL1; targets APPL2 to endosomes and acting in parallel to RAB5A (By similarity).

(Microbial infection) Interacts with human cytomegalovirus (HCMV).

Family&Domains:

A pair of annexin repeats may form one binding site for calcium and phospholipid.

Belongs to the annexin family.

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