HP Antibody | Affinity Biosciences

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Product:	HP Antibody
Catalog:	DF6467
Description:	Rabbit polyclonal antibody to HP
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Rabbit, Dog
Mol.Wt.:	42kDa; 45kD(Calculated).
Uniprot:	P00738
RRID:	AB_2838429

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Rabbit(80%), Dog(90%)

Clonality:

Polyclonal

Specificity:

HP Antibody detects endogenous levels of total HP.

RRID:

AB_2838429
Cite Format: Affinity Biosciences Cat# DF6467, RRID:AB_2838429.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Binding peptide; BP; Haptoglobin alpha chain; Haptoglobin alpha(1S) beta; Haptoglobin alpha(2FS) beta; Haptoglobin beta chain; Haptoglobin, alpha polypeptide; Haptoglobin, beta polypeptide; HP; HP2 ALPHA2; HP2ALPHA2; HPA1S; HPT; HPT_HUMAN; MGC111141; Zonulin;

Immunogens

Immunogen:

Uniprot:

P00738(HPT_HUMAN) >>Visit HPA database.

Gene(ID):

HP(3240)

Expression:

P00738 HPT_HUMAN:

Expressed by the liver and secreted in plasma.

Description:

This gene encodes a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson's disease, and a reduced incidence of Plasmodium falciparum malaria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Sequence:

P00738 HPT_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSALGAVIALLLWGQLFAVDSGNDVTDIADDGCPKPPEIAHGYVEHSVRYQCKNYYKLRTEGDGVYTLNDKKQWINKAVGDKLPECEADDGCPKPPEIAHGYVEHSVRYQCKNYYKLRTEGDGVYTLNNEKQWINKAVGDKLPECEAVCGKPKNPANPVQRILGGHLDAKGSFPWQAKMVSHHNLTTGATLINEQWLLTTAKNLFLNHSENATAKDIAPTLTLYVGKKQLVEIEKVVLHPNYSQVDIGLIKLKQKVSVNERVMPICLPSKDYAEVGRVGYVSGWGRNANFKFTDHLKYVMLPVADQDQCIRHYEGSTVPEKKTPKSPVGVQPILNEHTFCAGMSKYQEDTCYGDAGSAFAVHDLEEDTWYATGILSFDKSCAVAEYGVYVKVTSIQDWVQKTIAEN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Dog

Rabbit

Horse

Bovine

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P00738 As Substrate

P00738

Site	PTM Type	Source
Y55	Phosphorylation	Uniprot
Y56	Phosphorylation	Uniprot
Y66	Phosphorylation	Uniprot
Y114	Phosphorylation	Uniprot
Y115	Phosphorylation	Uniprot
Y125	Phosphorylation	Uniprot
K170	Acetylation	Uniprot
N184	N-Glycosylation	Uniprot
N207	N-Glycosylation	Uniprot
N211	N-Glycosylation	Uniprot
N241	N-Glycosylation	Uniprot
K251	Ubiquitination	Uniprot
C266	S-Nitrosylation	Uniprot
Y272	Phosphorylation	Uniprot
Y280	Phosphorylation	Uniprot
C309	S-Nitrosylation	Uniprot
K321	Acetylation	Uniprot
S326	Phosphorylation	Uniprot
C340	S-Nitrosylation	Uniprot
C381	S-Nitrosylation	Uniprot
Y386	Phosphorylation	Uniprot

Research Backgrounds

P00738_HPT_HUMAN

Function:

As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity, and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidly cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.

The uncleaved form of allele alpha-2 (2-2), known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.

Subcellular Location:

Secreted.

Tissue Specificity:

Expressed by the liver and secreted in plasma.

Subunit Structure:

Tetramer of two alpha and two beta chains; disulfide-linked. The hemoglobin/haptoglobin complex is composed of a haptoglobin dimer bound to two hemoglobin alpha-beta dimers. Interacts with CD163.

Family&Domains:

Belongs to the peptidase S1 family.

References

1). TMT-based quantitative proteomic analysis of hepatic tissue reveals the effects of dietary cyanidin-3-diglucoside-5-glucoside-rich extract on alleviating D-galactose-induced aging in mice. Journal of Proteomics, 2021 (PubMed: 33161165) [IF=3.3]

Application: WB Species: mice Sample: Liver tissues

Fig. 5. Western blot analysis of three differentially expressed proteins. Data are presented as means ± SD (n = 3 per group). Significance was determined by ANOVA followed by Tukey’s post hoc correction. *p < 0.05 vs NC, #p < 0.05 vs DG; Two-way ANOVA for groups (A) F = 13.8, df = 8; (B) F = 195.3, df = 8; (C) F = 23.1, df = 8.

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For Research Use Only.
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HP Antibody - #DF6467