NAT1 Antibody - #DF6459

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Product: NAT1 Antibody
Catalog: DF6459
Description: Rabbit polyclonal antibody to NAT1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Rabbit, Chicken
Mol.Wt.: 34kDa; 34kD(Calculated).
Uniprot: P18440
RRID: AB_2838421

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(90%), Rabbit(100%), Chicken(90%)
Clonality:
Polyclonal
Specificity:
NAT1 Antibody detects endogenous levels of total NAT1.
RRID:
AB_2838421
Cite Format: Affinity Biosciences Cat# DF6459, RRID:AB_2838421.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AAC1; ARY1_HUMAN; Arylamide acetylase 1; Arylamine N acetyltransferase 1; Arylamine N-acetyltransferase 1; mNAT; Monomorphic arylamine N acetyltransferase; Monomorphic arylamine N-acetyltransferase; N acetyltransferase type 1; N-acetyltransferase type 1; NAT 1; NAT-1; nat1; NATI;

Immunogens

Immunogen:
Uniprot:

P18440(ARY1_HUMAN) >>Visit HPA database.

Gene(ID):
NAT1(9)
Description:
This gene is one of two arylamine N-acetyltransferase (NAT) genes in the human genome, and is orthologous to the mouse and rat Nat2 genes. The enzyme encoded by this gene catalyzes the transfer of an acetyl group from acetyl-CoA to various arylamine and hydrazine substrates. This enzyme helps metabolize drugs and other xenobiotics, and functions in folate catabolism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
Sequence:
MDIEAYLERIGYKKSRNKLDLETLTDILQHQIRAVPFENLNIHCGDAMDLGLEAIFDQVVRRNRGGWCLQVNHLLYWALTTIGFETTMLGGYVYSTPAKKYSTGMIHLLLQVTIDGRNYIVDAGFGRSYQMWQPLELISGKDQPQVPCVFRLTEENGFWYLDQIRREQYIPNEEFLHSDLLEDSKYRKIYSFTLKPRTIEDFESMNTYLQTSPSSVFTSKSFCSLQTPDGVHCLVGFTLTHRRFNYKDNTDLIEFKTLSEEEIEKVLKNIFNISLQRKLVPKHGDRFFTI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Bovine
90
Chicken
90
Pig
0
Horse
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P18440 As Substrate

Site PTM Type Enzyme
M1 Acetylation
K18 Ubiquitination
T25 Phosphorylation
Y101 Phosphorylation
K185 Ubiquitination
K188 Ubiquitination
S191 Phosphorylation
Y208 Phosphorylation
S214 Phosphorylation
S221 Phosphorylation
T227 Phosphorylation
K247 Ubiquitination
K256 Ubiquitination
S259 Phosphorylation
K265 Ubiquitination
S274 Phosphorylation
T289 Phosphorylation

Research Backgrounds

Function:

Participates in the detoxification of a plethora of hydrazine and arylamine drugs. Catalyzes the N- or O-acetylation of various arylamine and heterocyclic amine substrates and is able to bioactivate several known carcinogens.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the arylamine N-acetyltransferase family.

Research Fields

· Human Diseases > Cancers: Overview > Chemical carcinogenesis.

· Metabolism > Biosynthesis of other secondary metabolites > Caffeine metabolism.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.

· Metabolism > Global and overview maps > Metabolic pathways.

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For Research Use Only.
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