PAH Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/3

Western blot analysis of extracts from RAW264.

DF6455 at 1/100 staining Human ovarian cancer by IHC-P.

Product:	PAH Antibody
Catalog:	DF6455
Description:	Rabbit polyclonal antibody to PAH
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	52kDa, 70 kDa; 52kD(Calculated).
Uniprot:	P00439
RRID:	AB_2838418

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(88%), Horse(100%), Sheep(88%), Rabbit(100%), Dog(100%), Chicken(88%), Xenopus(88%)

Clonality:

Polyclonal

Specificity:

PAH Antibody detects endogenous levels of total PAH.

RRID:

AB_2838418
Cite Format: Affinity Biosciences Cat# DF6455, RRID:AB_2838418.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

PAH; PH; PH4H_HUMAN; Phe 4 monooxygenase; Phe-4-monooxygenase; Phenylalanine 4 hydroxylase; Phenylalanine hydroxylase; Phenylalanine-4-hydroxylase; PKU; PKU1;

Immunogens

Immunogen:

Uniprot:

P00439(PH4H_HUMAN) >>Visit HPA database.

Gene(ID):

PAH(5053)

Description:

PAH encodes the enzyme phenylalanine hydroxylase that is the rate-limiting step in phenylalanine catabolism. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria.

Sequence:

P00439 PH4H_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSTAVLENPGLGRKLSDFGQETSYIEDNCNQNGAISLIFSLKEEVGALAKVLRLFEENDVNLTHIESRPSRLKKDEYEFFTHLDKRSLPALTNIIKILRHDIGATVHELSRDKKKDTVPWFPRTIQELDRFANQILSYGAELDADHPGFKDPVYRARRKQFADIAYNYRHGQPIPRVEYMEEEKKTWGTVFKTLKSLYKTHACYEYNHIFPLLEKYCGFHEDNIPQLEDVSQFLQTCTGFRLRPVAGLLSSRDFLGGLAFRVFHCTQYIRHGSKPMYTPEPDICHELLGHVPLFSDRSFAQFSQEIGLASLGAPDEYIEKLATIYWFTVEFGLCKQGDSIKAYGAGLLSSFGELQYCLSEKPKLLPLELEKTAIQNYTVTEFQPLYYVAESFNDAKEKVRNFAATIPRPFSVRYDPYTQRIEVLDNTQQLKILADSINSEIGILCSALQKIK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Dog

100

Rabbit

100

Bovine

Sheep

Xenopus

Chicken

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P00439 As Substrate

P00439

Site	PTM Type	Enzyme	Source
S2	Phosphorylation		Uniprot
T3	Phosphorylation		Uniprot
S16	Phosphorylation	P17612 (PRKACA)	Uniprot
S87	Phosphorylation		Uniprot
T92	Phosphorylation		Uniprot
T117	Phosphorylation		Uniprot
S436	Phosphorylation		Uniprot

Research Backgrounds

P00439_PH4H_HUMAN

Function:

Catalyzes the hydroxylation of L-phenylalanine to L-tyrosine.

PTMs:

Phosphorylation at Ser-16 increases basal activity and facilitates activation by the substrate phenylalanine.

Subunit Structure:

Homodimer and homotetramer.

Family&Domains:

Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.

Research Fields

· Metabolism > Amino acid metabolism > Phenylalanine metabolism.

· Metabolism > Amino acid metabolism > Phenylalanine, tyrosine and tryptophan biosynthesis.

· Metabolism > Metabolism of cofactors and vitamins > Folate biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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PAH Antibody - #DF6455