TNF beta Antibody - #DF6453

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Product: TNF beta Antibody
Catalog: DF6453
Description: Rabbit polyclonal antibody to TNF beta
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Horse, Dog
Mol.Wt.: 22kDa; 22kD(Calculated).
Uniprot: P01374
RRID: AB_2838416

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Horse(89%), Dog(89%)
Clonality:
Polyclonal
Specificity:
TNF beta Antibody detects endogenous levels of total TNF beta.
RRID:
AB_2838416
Cite Format: Affinity Biosciences Cat# DF6453, RRID:AB_2838416.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

LTalpha; DIF; LT alpha; LT; LT-alpha; Lta; Lymphotoxin alpha (TNF superfamily, member 1); Lymphotoxin alpha; Lymphotoxin-alpha; TNF B; TNF superfamily member 1; TNF, lymphocyte-derived; TNF-beta; TNFB; TNFB_HUMAN; TNFbeta; TNFSF1; TNLG1E; Tumor necrosis factor beta; tumor necrosis factor ligand 1E; Tumor necrosis factor ligand superfamily member 1;

Immunogens

Immunogen:
Uniprot:

P01374(TNFB_HUMAN) >>Visit HPA database.

Gene(ID):
LTA(4049)
Description:
Lymphotoxin-α (LT-α), also known as TNF–β, is a member of the TNF superfamily of proteins (1). NK cells, T cells, B cells, and lymphoid tissue-inducer cells express LT-α (1). LT-α can be secreted as a soluble homotrimer or form membrane bound heterotrimers with lymphotoxin-β (LT α1β2 or LT α2β1) which can be cleaved from the cell surface by matrix metalloproteases (1,2). Soluble LT-α binds to and signals through TNFR1/TNFR2, activating the canonical NF-κB pathway (1). In contrast, LT α1β2 heterodimers bind to the LTβR receptor and activate the noncanonical NF-κB pathway (1). As a result, LT-α and TNF-α have overlapping functions. Soluble LT-α and LT α1β2 play key roles in lymphangiogenesis (3). The LT α1β2/LTβR axis is essential for the development of lymphoid tissue (1,3).
Sequence:
MTPPERLFLPRVCGTTLHLLLLGLLLVLLPGAQGLPGVGLTPSAAQTARQHPKMHLAHSTLKPAAHLIGDPSKQNSLLWRANTDRAFLQDGFSLSNNSLLVPTSGIYFVYSQVVFSGKAYSPKATSSPLYLAHEVQLFSSQYPFHVPLLSSQKMVYPGLQEPWLHSMYHGAAFQLTQGDQLSTHTDGIPHLVLSPSTVFFGAFAL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
89
Dog
89
Pig
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P01374 As Substrate

Site PTM Type Enzyme
T41 O-Glycosylation

Research Backgrounds

Function:

Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and is cytotoxic for a wide range of tumor cells in vitro and in vivo.

Subcellular Location:

Secreted. Membrane.
Note: The homotrimer is secreted. The heterotrimer is membrane-associated.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homotrimer, and heterotrimer of either two LTB and one LTA subunits or (less prevalent) two LTA and one LTB subunits. Interacts with TNFRSF14.

Family&Domains:

Belongs to the tumor necrosis factor family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Type I diabetes mellitus.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

References

1). Elevated lymphotoxin-α (TNFβ) is associated with intervertebral disc degeneration. BMC Musculoskeletal Disorders, 2021 (PubMed: 33441130) [IF=2.3]

Application: WB    Species: human    Sample:

Fig. 1 Images of intervertebral disc degeneration and detection of LTα in human NP tissues. (A) Upper panel: normal disc in a MRI T2 image; bottom panel: degenerated disc in a MRI T2 image. (B) Protein lysates from NP from the control and degeneration groups were blotted with anti-LTα and anti-GAPDH antibodies. (C) Quantitative analysis of LTα protein levels as determined in “B”. (D) Concentration of LTα in NP tissue determined by ELISA. (E) LTα mRNA production in NP tissue determined by RT-qPCR. Control group: relatively healthy disc samples from patients with idiopathic scoliosis or spinal trauma; degeneration group: degenerated disc samples from patients with lumbar spinal stenosis or discogenic low back pain. The GAPDH housekeeping gene/protein was used as the internal control. **. p 

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