NQO1 Antibody | Affinity Biosciences

Product:	NQO1 Antibody
Catalog:	DF6437
Description:	Rabbit polyclonal antibody to NQO1
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	31kDa; 31kD(Calculated).
Uniprot:	P15559
RRID:	AB_2838400

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-400
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(89%), Bovine(89%), Horse(89%), Sheep(89%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

NQO1 Antibody detects endogenous levels of total NQO1.

RRID:

AB_2838400
Cite Format: Affinity Biosciences Cat# DF6437, RRID:AB_2838400.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Azoreductase; Cytochrome b 5 reductase; DHQU; DIA 4; DIA4; Diaphorase (NADH/NADPH) (cytochrome b 5 reductase); Diaphorase (NADH/NADPH); Diaphorase 4; Dioxin inducible 1; DT diaphorase; DT-diaphorase; DTD; Menadione reductase; NAD(P)H dehydrogenase [quinone] 1; NAD(P)H dehydrogenase quinone 1; NAD(P)H menadione oxidoreductase 1 dioxin inducible; NAD(P)H quinone dehydrogenase 1; NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1; NAD(P)H:menadione oxidoreductase 1; NAD(P)H:Quinone acceptor oxidoreductase type 1; NAD(P)H:quinone oxidoreductase 1; NAD(P)H:quinone oxireductase; NMOR 1; NMOR I; NMOR1; NMORI; NQO 1; NQO1; NQO1_HUMAN; Phylloquinone reductase; QR 1; QR1; Quinone reductase 1;

Immunogens

Immunogen:

Uniprot:

P15559(NQO1_HUMAN) >>Visit HPA database.

Gene(ID):

NQO1(1728)

Description:

NAD(P)H:quinone oxidoreductase 1 (NQO1) is a flavoprotein that catalyzes the two-electron reduction of quinones and their derivatives (1,2). This enzyme protects cells against redox cycling and oxidative stress (1,3). The expression of NQO1 is increased in liver, colon and breast tumors and non-small cell lung cancer (NSCLC) compared with the normal tissues (1,2). Moreover, expression levels are also elevated in developing tumors, suggesting a role for NQ01 in the prevention of tumor development (1). Studies on NQO1 knockout mice suggest that the lack of NQO1 enzymatic activity changes intracellular redox states resulting in a reduction in apoptosis, which in turns leads to myeloid hyperplasia of bone marrow (2).

Sequence:

P15559 NQO1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVGRRALIVLAHSERTSFNYAMKEAAAAALKKKGWEVVESDLYAMNFNPIISRKDITGKLKDPANFQYPAESVLAYKEGHLSPDIVAEQKKLEAADLVIFQFPLQWFGVPAILKGWFERVFIGEFAYTYAAMYDKGPFRSKKAVLSITTGGSGSMYSLQGIHGDMNVILWPIQSGILHFCGFQVLEPQLTYSIGHTPADARIQILEGWKKRLENIWDETPLYFAPSSLFDLNFQAGFLMKKEVQDEEKNKKFGLSVGHHLGKSIPTDNQIKARK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Dog

100

Pig

Horse

Bovine

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P15559 As Substrate

P15559

Site	PTM Type	Source
S13	Phosphorylation	Uniprot
Y20	Phosphorylation	Uniprot
K23	Ubiquitination	Uniprot
K31	Acetylation	Uniprot
K31	Ubiquitination	Uniprot
K33	Ubiquitination	Uniprot
S40	Phosphorylation	Uniprot
Y43	Phosphorylation	Uniprot
K54	Ubiquitination	Uniprot
K59	Acetylation	Uniprot
K59	Ubiquitination	Uniprot
K61	Ubiquitination	Uniprot
Y68	Phosphorylation	Uniprot
Y76	Phosphorylation	Uniprot
K77	Acetylation	Uniprot
K77	Ubiquitination	Uniprot
S82	Phosphorylation	Uniprot
K90	Ubiquitination	Uniprot
K91	Ubiquitination	Uniprot
Y127	Phosphorylation	Uniprot
Y129	Phosphorylation	Uniprot
Y133	Phosphorylation	Uniprot
K135	Ubiquitination	Uniprot
K209	Acetylation	Uniprot
K209	Ubiquitination	Uniprot
K210	Acetylation	Uniprot
K210	Ubiquitination	Uniprot
K241	Ubiquitination	Uniprot
K248	Ubiquitination	Uniprot
K251	Sumoylation	Uniprot
K251	Ubiquitination	Uniprot
S255	Phosphorylation	Uniprot
K262	Acetylation	Uniprot
K262	Ubiquitination	Uniprot
K271	Sumoylation	Uniprot
K271	Ubiquitination	Uniprot

Research Backgrounds

P15559_NQO1_HUMAN

Function:

The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.

Subcellular Location:

Cytoplasm.

Subunit Structure:

Homodimer. Interacts with PDLIM4 isoform 2; this interaction stabilizes PDLIM4 isoform 2 in response to oxidative stress and protects it from ubiquitin-independent degradation by the core 20S proteasome.

Family&Domains:

Belongs to the NAD(P)H dehydrogenase (quinone) family.

Research Fields

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma. (View pathway)

· Metabolism > Metabolism of cofactors and vitamins > Ubiquinone and other terpenoid-quinone biosynthesis.

References

1). Mitochondria-targeted supramolecular coordination container encapsulated with exogenous itaconate for synergistic therapy of joint inflammation. Theranostics, 2023 (PubMed: 35547753) [IF=12.4]

2). Cytotoxicity of adducts formed between quercetin and methylglyoxal in PC-12 cells. Food Chemistry, 2021 (PubMed: 33706136) [IF=8.8]

Application: WB Species: Rat Sample: PC-12 cells

Fig. 5. Effect of treatments of MGO, Que-mono-MGO, and Que-di-MGO on the expression levels of apoptotic markers and components of AKT and Nrf2-HO-1/NQO-1 signaling pathways. Significant differences (p < 0.05) between samples of different treatments are marked with different letters on each column.

3). Total flavonoids of Inula japonica alleviated the inflammatory response and oxidative stress in LPS-induced acute lung injury via inhibiting the sEH activity: Insights from lipid metabolomics. PHYTOMEDICINE, 2022 (PubMed: 36150346) [IF=7.9]

4). Moschus exerted protective activity against H2O2-induced cell injury in PC12 cells through regulating Nrf-2/ARE signaling pathways. Biomedicine & Pharmacotherapy, 2023 (PubMed: 36708701) [IF=7.5]

Application: WB Species: Rat Sample: PC12 cells

Fig. 8. A. WB analysis was performed to observe Moschus’ effects on Keap1, NQO-1, Nrf-2, and OH-1 protein levels in the injured model. PC12 cells were processed with 70 μM H2O2 after pretreatment with a specific concentration of Moschus. (Model: 70 μM H2O2-treated PC12 cells, VC: 100 μM L-ascorbate-treated PC12 cells). (A) The ratio between Keap1 and β-actin (n = 3). (B) The ratio between NQO-1 and β-actin (n = 3). (C) The ratio between Nrf-2 and β-actin (n = 3). (D) The ratio between OH-1 and β-actin (n = 3). 1: Control 2: Model 3: Moschus-0.05 mg/ml 4: Moschus-0.1 mg/ml 5: Moschus-0.15 mg/ml 6: VC. Dates were represented as means ± SEM. * p < 0.05, * * p < 0.01, and * ** p < 0.001 vs the model group.

5). The effect of monotropein on alleviating cisplatin-induced acute kidney injury by inhibiting oxidative damage, inflammation and apoptosis. BIOMEDICINE & PHARMACOTHERAPY, 2020 (PubMed: 32574971) [IF=7.5]

Application: WB Species: mouse Sample: kidney

Fig. 2.| Pretreatment with monotropein protected against renal oxidative stress induced by cisplatin. The level of serum GSH(A). The level of serum MDA(B). The level of serum SOD(C). The level of serum CAT(D). The protein expressions of Nrf2, HO-1 and NQO1 (E) were detected by Western blot in kidney tissue.

6). Med1 inhibits ferroptosis and alleviates liver injury in acute liver failure via Nrf2 activation. Cell & bioscience, 2024 (PubMed: 38678227) [IF=7.5]

7). Developing an advanced diagnostic model for hepatocellular carcinoma through multi-omics integration leveraging diverse cell-death patterns. Frontiers in immunology, 2024 (PubMed: 39044829) [IF=7.3]

Application: IHC Species: Human Sample: HCC cell

Figure 7 The expression pattern of seven key genes in the scRNA-seq and stRNA-seq data. (A-G) The violin plots on the left side illustrate the expression levels of such seven key genes in the HCC cell subsets of two independent datasets of GSE149614 and GSE151530, while the violin plots on the right side present their corresponding expression patterns in the normal hepatocytes, HCC cells, and proliferative HCC cells in the external validation scRNA-seq dataset. Their spatial expression distribution in normal liver, ICB responders, and ICB non-responders is shown in the right panel. (H) IHC analysis shows upregulated TRIB3 and NQO1 expression in tissue samples of HCC patients relative to the normal controls. * P

8). Echinacoside alleviates hypobaric hypoxia-induced memory impairment in C57 mice. PHYTOTHERAPY RESEARCH, 2019 (PubMed: 30768741) [IF=7.2]

Application: WB Species: mouse Sample: Hippocampi

FIGURE 7 |Effects of different doses of echinacoside (ECH) on the expressions of nuclear factor E2 p45‐related factor 2 (Nrf2), heme oxygenase‐1 (HO‐1), NAD(P)H:quinone oxidoreductase 1 (NQO1), and γ‐glutamyl cysteine synthetase (γ‐GCS) in the mice exposed to hypobaric hypoxia in protein level. Hippocampi of the mice were collected, and the protein levels of Nrf2 (cytoplasmic and nuclear), HO‐1, NQO1, and γ‐GCS were evaluated by Western blot. *p < 0.05, **p < 0.01 versus control; #p < 0.05, ##p < 0.01 versus hypoxia (HYP)

9). A quantitative proteomic study reveals oxidative stress and synapse-related proteins contributed to TDCIPP exposure induced neurotoxicity. Ecotoxicology and environmental safety, 2024 (PubMed: 38262093) [IF=6.8]

Application: WB Species: Rat Sample:

Fig. 5. Dysregulated proteins induced by TDCIPP exposure were analyzed by heatmap and Western blot. (A) The heatmap is clustered in rows and the values are protein expression abundance, with blue representing down-regulated proteins and red representing up-regulated proteins. Western blot analysis and quantification of PKN1 (B and F), GSTM4 (C and G), BMAL1 (D and H), NQO1 (E and I) expression levels. Data was shown Mean ± SEM. * , p

10). The protective effect of forsythiaside A on 3,5-diethoxycarbonyl-1,4-dihydrocollidine-induced cholestatic liver injury in mice: Based on targeted metabolomics and molecular biology technology. Biochimica et biophysica acta. Molecular basis of disease, 2023 (PubMed: 37523877) [IF=6.2]

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NQO1 Antibody - #DF6437