NPY Antibody | Affinity Biosciences

IHC
Cites

1/5

DF6431 at 1/100 staining Rat brain tissue by IHC-P.

Fig.

Figure 1.

Product:	NPY Antibody
Catalog:	DF6431
Description:	Rabbit polyclonal antibody to NPY
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	11kDa; 11kD(Calculated).
Uniprot:	P01303
RRID:	AB_2838394

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

NPY Antibody detects endogenous levels of total NPY.

RRID:

AB_2838394
Cite Format: Affinity Biosciences Cat# DF6431, RRID:AB_2838394.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

C-flanking peptide of NPY; CPON; Neuropeptide tyrosine; Neuropeptide Y precursor; NPY; NPY_HUMAN; Pro neuropeptide Y; PYY 4; PYY4; Y Neuropeptide;

Immunogens

Immunogen:

Uniprot:

P01303(NPY_HUMAN) >>Visit HPA database.

Gene(ID):

NPY(4852)

Expression:

P01303 NPY_HUMAN:

One of the most abundant peptides in the nervous system. Also found in some chromaffin cells of the adrenal medulla.

Description:

The NPY hormone family consists of NPY, PP and peptide YY. NPY (neuropeptide Y) is a 36 amino acid protein that consists of a polyproline stretch followed by an amphipathic a-helix. NPY shares a 50% amino acid homology with pancreatic polypeptide gene (PP). NPY is expressed throughout the central and peripheral nervous system, particularly in the deep layers of the cortex and smaller cell bodies in the white matter. NPY interacts with the Y-receptor family of G protein-coupled receptors. NPY interacts with NPY receptor Y1 to increase corticotropin levels and decrease noradrenaline levels in the hypothalamus. Through interactions in the hypothalus, NPY plays important roles in the regulation of energy balance by stimulating food intake. NPY favors energy storage by increasing lipoprotein lipase activity in white adipose tissue. A Leucine 7 to Proline 7 polymorphism has been implicated in an increase in alcohol preference, and decrease in cholesterol metabolism.

Sequence:

P01303 NPY_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLGNKRLGLSGLTLALSLLVCLGALAEAYPSKPDNPGEDAPAEDMARYYSALRHYINLITRQRYGKRSSPETLISDLLMRESTENVPRTRLEDPAMW

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Zebrafish

100

Chicken

100

Rabbit

100

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P01303 As Substrate

P01303

Site	PTM Type	Source
Y48	Phosphorylation	Uniprot
Y49	Phosphorylation	Uniprot
S50	Phosphorylation	Uniprot
Y55	Phosphorylation	Uniprot
S75	Phosphorylation	Uniprot
S82	Phosphorylation	Uniprot
T83	Phosphorylation	Uniprot

Research Backgrounds

P01303_NPY_HUMAN

Function:

NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone.

PTMs:

The neuropeptide Y form is cleaved at Pro-30 by the prolyl endopeptidase FAP (seprase) activity (in vitro).

Subcellular Location:

Secreted. Cytoplasmic vesicle>Secretory vesicle>Neuronal dense core vesicle.

Tissue Specificity:

One of the most abundant peptides in the nervous system. Also found in some chromaffin cells of the adrenal medulla.

Family&Domains:

Belongs to the NPY family.

Research Fields

· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)

· Human Diseases > Substance dependence > Alcoholism.

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

References

1). Metabolic regulation of Ganoderma lucidum extracts in high sugar and fat diet-induced obese mice by regulating the gut-brain axis. Journal of Functional Foods, 2020 [IF=5.6]

2). Neuropeptide Y upregulates Runx2 and osterix and enhances osteogenesis in mouse MC3T3‑E1 cells via an autocrine mechanism. Molecular Medicine Reports, 2020 [IF=3.4]

Application: WB Species: Mouse Sample:

Figure 1. - Expression of NPY mRNA and protein in MC3T3-E1 cells. (A) Effects of siRNA inhibition on the expression of NPY mRNA at 48 h. **P

3). Chronic heat stress alters hypothalamus integrity, the serum indexes and attenuates expressions of hypothalamic appetite genes in broilers. Journal of Thermal Biology, 2019 (PubMed: 30975407) [IF=2.7]

Application: IF/ICC Species: broilers Sample: hypothalamus

Fig. 5. |Effect of chronic heat stress on protein levels of NPY in the hypothalamus of broilers after 14 days heat stress based on the whole-brain immunofluorescent method of the control group (A), heat stress group (B), pair-fed group (C). NPY, neuropeptide Y. White arrows on the right row panels indicates NPY proteins which colors are red.

4). Electroacupuncture alleviates Parkinson disease and regulates the expression of brain-gut peptides. EXPERIMENTAL ANIMALS, 2020 (PubMed: 32669479) [IF=2.4]

Application: WB Species: rat Sample: brain

Fig. 5.| Effects of EA on the levels of brain-gut peptides. A. The expression of NPY, CCK, and SST in the brain substantia nigra of rats from NC group, Sham group, 6-OHDA group, and 6-OHDA+EA group was determined by Western blot. B. The levels of NPY,CCK, SST, MTL, GAS, and PYY in the serum of rats from each group were detected by ELSA. Data are expressed as mean ±SD. *P<0.05, **P<0.01.

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NPY Antibody - #DF6431