TLR8 Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/6

Western blot analysis of extracts from rat brain, using TLR8 Antibody.

Western blot analysis of extracts from HepG2 cells(LPS 4h treatment), using TLR8 Antibody.

DF6426 at 1/100 staining Rat muscle tissue by IHC-P.

DF6426 at 1/100 staining Mouse liver tissue by IHC-P.

DF6426 at 1/100 staining Mouse stomach tissue by IHC-P.

DF6426 staining HeLa cells by ICC/IF.

Product:	TLR8 Antibody
Catalog:	DF6426
Description:	Rabbit polyclonal antibody to TLR8
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Dog
Mol.Wt.:	115kDa; 120kD(Calculated).
Uniprot:	Q9NR97
RRID:	AB_2838389

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(86%), Bovine(86%), Horse(86%), Dog(86%)

Clonality:

Polyclonal

Specificity:

TLR8 Antibody detects endogenous levels of total TLR8.

RRID:

AB_2838389
Cite Format: Affinity Biosciences Cat# DF6426, RRID:AB_2838389.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CD 288; CD288; CD288 antigen; MGC119599; MGC119600; TLR 8; Tlr8; TLR8_HUMAN; Toll like receptor 8; Toll-like receptor 8;

Immunogens

Immunogen:

Uniprot:

Q9NR97(TLR8_HUMAN) >>Visit HPA database.

Gene(ID):

TLR8(51311)

Expression:

Q9NR97 TLR8_HUMAN:

Detected in brain, heart, lung, liver, placenta, in monocytes, and at lower levels in CD11c+ immature dendritic cells.

Description:

The Toll-like Receptors (TLR) are a family of human receptors that share homology with the Drosophila Toll Receptors, which are involved in mediating dorsoventral polarization in developing Drosophila embryos and participate in host immunity. The TLR family members are characterized by a highly conserved Toll homology (TH) domain, which is essential for Toll-induced signal transductions. TLRs are type I transmembrane receptors that contain an extracellular domain consisting of several leucine-rich regions and a single cytoplasmic Toll/IL-1R like domain. Three TLR family members, TLR7, TLR8 and TLR9, belong to a subfamily of TLRs which are differentially expressed. TLR7 is expressed in lung, placenta and spleen. TLR8 is expressed in lung and peripheral blood leukocytes, and TLR9 is predominantly expressed in spleen, lymph nodes, bone marrow and peripheral blood leukocytes. TLR7, TLR8 and TLR9 stimulate the NF B signaling pathway, suggesting that they play a role in the immune response.

Sequence:

Q9NR97 TLR8_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MENMFLQSSMLTCIFLLISGSCELCAEENFSRSYPCDEKKQNDSVIAECSNRRLQEVPQTVGKYVTELDLSDNFITHITNESFQGLQNLTKINLNHNPNVQHQNGNPGIQSNGLNITDGAFLNLKNLRELLLEDNQLPQIPSGLPESLTELSLIQNNIYNITKEGISRLINLKNLYLAWNCYFNKVCEKTNIEDGVFETLTNLELLSLSFNSLSHVPPKLPSSLRKLFLSNTQIKYISEEDFKGLINLTLLDLSGNCPRCFNAPFPCVPCDGGASINIDRFAFQNLTQLRYLNLSSTSLRKINAAWFKNMPHLKVLDLEFNYLVGEIASGAFLTMLPRLEILDLSFNYIKGSYPQHINISRNFSKLLSLRALHLRGYVFQELREDDFQPLMQLPNLSTINLGINFIKQIDFKLFQNFSNLEIIYLSENRISPLVKDTRQSYANSSSFQRHIRKRRSTDFEFDPHSNFYHFTRPLIKPQCAAYGKALDLSLNSIFFIGPNQFENLPDIACLNLSANSNAQVLSGTEFSAIPHVKYLDLTNNRLDFDNASALTELSDLEVLDLSYNSHYFRIAGVTHHLEFIQNFTNLKVLNLSHNNIYTLTDKYNLESKSLVELVFSGNRLDILWNDDDNRYISIFKGLKNLTRLDLSLNRLKHIPNEAFLNLPASLTELHINDNMLKFFNWTLLQQFPRLELLDLRGNKLLFLTDSLSDFTSSLRTLLLSHNRISHLPSGFLSEVSSLKHLDLSSNLLKTINKSALETKTTTKLSMLELHGNPFECTCDIGDFRRWMDEHLNVKIPRLVDVICASPGDQRGKSIVSLELTTCVSDVTAVILFFFTFFITTMVMLAALAHHLFYWDVWFIYNVCLAKVKGYRSLSTSQTFYDAYISYDTKDASVTDWVINELRYHLEESRDKNVLLCLEERDWDPGLAIIDNLMQSINQSKKTVFVLTKKYAKSWNFKTAFYLALQRLMDENMDVIIFILLEPVLQHSQYLRLRQRICKSSILQWPDNPKAEGLFWQTLRNVVLTENDSRYNNMYVDSIKQY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Bovine

Dog

Rabbit

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NR97 As Substrate

Q9NR97

Site	PTM Type	Source
N80	N-Glycosylation	Uniprot
N88	N-Glycosylation	Uniprot
N293	N-Glycosylation	Uniprot
S345	Phosphorylation	Uniprot
Y348	Phosphorylation	Uniprot
S368	Phosphorylation	Uniprot
N395	N-Glycosylation	Uniprot
N511	N-Glycosylation	Uniprot
N546	N-Glycosylation	Uniprot
N590	N-Glycosylation	Uniprot
N640	N-Glycosylation	Uniprot
N680	N-Glycosylation	Uniprot
T762	Phosphorylation	Uniprot
S765	Phosphorylation	Uniprot
Y880	Phosphorylation	Uniprot
Y886	Phosphorylation	Uniprot
S935	Phosphorylation	Uniprot
S939	Phosphorylation	Uniprot
Y1030	Phosphorylation	Uniprot

Research Backgrounds

Q9NR97_TLR8_HUMAN

Function:

Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.

Subcellular Location:

Membrane>Single-pass type I membrane protein.

Tissue Specificity:

Detected in brain, heart, lung, liver, placenta, in monocytes, and at lower levels in CD11c+ immature dendritic cells.

Subunit Structure:

Interacts with MYD88 via their respective TIR domains. Interacts with UNC93B1 (By similarity). Homodimer. Interacts with BTK. Interacts with SMPDL3B (By similarity).

Family&Domains:

Belongs to the Toll-like receptor family.

Research Fields

· Organismal Systems > Immune system > Toll-like receptor signaling pathway. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

TLR8 Antibody - #DF6426