Factor X Antibody | Affinity Biosciences

WB
IF/ICC

1/3

Western blot analysis of extracts from A2780 cells, using F10 Antibody.

Western blot analysis of extracts from Myeloma cells, using F10 Antibody.

Product:	Factor X Antibody
Catalog:	DF6416
Description:	Rabbit polyclonal antibody to Factor X
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	55kDa, 65 kDa; 55kD(Calculated).
Uniprot:	P00742
RRID:	AB_2838379

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

Factor X Antibody detects endogenous levels of total Factor X.

RRID:

AB_2838379
Cite Format: Affinity Biosciences Cat# DF6416, RRID:AB_2838379.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Activated factor Xa heavy chain; Coagulation factor X; F10; FA10_HUMAN; factor Xa; FX; FXA; Prothrombinase; Stuart factor; Stuart Prower factor; Stuart-Prower factor;

Immunogens

Immunogen:

Uniprot:

P00742(FA10_HUMAN) >>Visit HPA database.

Gene(ID):

F10(2159)

Expression:

P00742 FA10_HUMAN:

Plasma; synthesized in the liver.

Description:

This gene encodes the vitamin K-dependent coagulation factor X of the blood coagulation cascade. This factor undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by 1 or more disulfide bonds; the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrisic pathway), or by factor VIIa (in the extrinsic pathway). The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting. Mutations of this gene result in factor X deficiency, a hemorrhagic condition of variable severity. [provided by RefSeq, Jul 2008]

Sequence:

P00742 FA10_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGRPLHLVLLSASLAGLLLLGESLFIRREQANNILARVTRANSFLEEMKKGHLERECMEETCSYEEAREVFEDSDKTNEFWNKYKDGDQCETSPCQNQGKCKDGLGEYTCTCLEGFEGKNCELFTRKLCSLDNGDCDQFCHEEQNSVVCSCARGYTLADNGKACIPTGPYPCGKQTLERRKRSVAQATSSSGEAPDSITWKPYDAADLDPTENPFDLLDFNQTQPERGDNNLTRIVGGQECKDGECPWQALLINEENEGFCGGTILSEFYILTAAHCLYQAKRFKVRVGDRNTEQEEGGEAVHEVEVVIKHNRFTKETYDFDIAVLRLKTPITFRMNVAPACLPERDWAESTLMTQKTGIVSGFGRTHEKGRQSTRLKMLEVPYVDRNSCKLSSSFIITQNMFCAGYDTKQEDACQGDSGGPHVTRFKDTYFVTGIVSWGEGCARKGKYGIYTKVTAFLKWIDRSMKTRGLPKAKSHAPEVITSSPLK

PTMs - P00742 As Substrate

P00742

Site	PTM Type	Source
T199	O-Glycosylation	Uniprot
T211	O-Glycosylation	Uniprot
Y384	Phosphorylation	Uniprot
Y407	Phosphorylation	Uniprot
T483	O-Glycosylation	Uniprot

Research Backgrounds

P00742_FA10_HUMAN

Function:

Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.

PTMs:

The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.

N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.

The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway).

The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.

Subcellular Location:

Secreted.

Tissue Specificity:

Plasma; synthesized in the liver.

Subunit Structure:

The two chains are formed from a single-chain precursor by the excision of two Arg residues and are held together by 1 or more disulfide bonds. Forms a heterodimer with SERPINA5.

Family&Domains:

Belongs to the peptidase S1 family.

Research Fields

· Organismal Systems > Immune system > Complement and coagulation cascades. (View pathway)

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Factor X Antibody - #DF6416