AGER/RAGE Antibody | Affinity Biosciences

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Product:	AGER/RAGE Antibody
Catalog:	DF6405
Description:	Rabbit polyclonal antibody to AGER/RAGE
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Horse, Rabbit
Mol.Wt.:	43~60kDa; 43kD(Calculated).
Uniprot:	Q15109
RRID:	AB_2838368

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(88%), Horse(88%), Rabbit(100%)

Clonality:

Polyclonal

Specificity:

AGER/RAGE Antibody detects endogenous levels of total AGER/RAGE.

RRID:

AB_2838368
Cite Format: Affinity Biosciences Cat# DF6405, RRID:AB_2838368.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Advanced glycosylation end product-specific receptor; Ager; MGC2235; RAGE_HUMAN; Receptor for advanced glycosylation end products;

Immunogens

Immunogen:

Uniprot:

Q15109(RAGE_HUMAN) >>Visit HPA database.

Gene(ID):

AGER(177)

Expression:

Q15109 RAGE_HUMAN:

Endothelial cells.

Description:

The receptor for advanced glycation end products (RAGE) is member of the immunoglobulin (Ig) superfamily. It can be expressed as full-length, membrane-bound RAGE isoform 1 or as a secreted sRAGE protein that lacks a transmembrane domain (1). RAGE is detected during early developmental stages and in the lung under normal physiological conditions (2) and is upregulated at sites of inflammation (3). Advanced glycation end products (AGEs) and a variety of other ligands interact with this receptor (1). Ligand binding activates full-length RAGE and initiates downstream signaling pathways that include activation of NF-κB, which leads to production of pro-inflammatory cytokines and inflammation (4). Activation of these pathways has been implicated in various disease states including Alzheimer disease, diabetes, arthritis, and atherosclerosis (4). Soluble RAGE can competitively bind RAGE ligands in the extracellular environment, which prevents ligand interaction with full-length RAGE at the cell surface (1).

Sequence:

Q15109 RAGE_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAGTAVGAWVLVLSLWGAVVGAQNITARIGEPLVLKCKGAPKKPPQRLEWKLNTGRTEAWKVLSPQGGGPWDSVARVLPNGSLFLPAVGIQDEGIFRCQAMNRNGKETKSNYRVRVYQIPGKPEIVDSASELTAGVPNKVGTCVSEGSYPAGTLSWHLDGKPLVPNEKGVSVKEQTRRHPETGLFTLQSELMVTPARGGDPRPTFSCSFSPGLPRHRALRTAPIQPRVWEPVPLEEVQLVVEPEGGAVAPGGTVTLTCEVPAQPSPQIHWMKDGVPLPLPPSPVLILPEIGPQDQGTYSCVATHSSHGPQESRAVSISIIEPGEEGPTAGSVGGSGLGTLALALGILGGLGTAALLIGVILWQRRQRRGEERKAPENQEEEEERAELNQSEEPEAGESSTGGP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Pig

Horse

Bovine

Sheep

Dog

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15109 As Substrate

Q15109

Site	PTM Type	Source
S391	Phosphorylation	Uniprot
S399	Phosphorylation	Uniprot
S400	Phosphorylation	Uniprot

Research Backgrounds

Q15109_RAGE_HUMAN

Function:

Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. Can also bind oligonucleotides.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Secreted.

Cell membrane>Single-pass type I membrane protein.

Tissue Specificity:

Endothelial cells.

Subunit Structure:

Interacts with S100A1 and APP (By similarity). Interacts with S100B, S100A12 and S100A14. Constitutive homodimer; disulfide-linked.

References

1). GLP-1 mediates the neuroprotective action of crocin against cigarette smoking-induced cognitive disorders via suppressing HMGB1-RAGE/TLR4-NF-κB pathway. International Immunopharmacology, 2022 (PubMed: 35785730) [IF=5.6]

Application: WB Species: Rat Sample: hippocampal

Fig. 5. Effect of crocin on hippocampal HMGB-1 level and expression as well as RAGE expression in a rat model of CS-induced cognitive disorders. (A) HMGB1 level (pg/g tissue); n = 6, (B) western blot showing HMGB1 and RAGE expression, (C) bar graph reflecting HMGB1 expression, and (D) bar graph reflecting RAGE expression. Protein expression level was normalized to β-actin and shown relative to that of control; n = 3. Values were expressed as means ± SD. Significant difference were presented at *p < 0.05 and ***p < 0.001; using One-way ANOVA followed by Tukey's Honestly Significant Difference (HSD) post-hoc. C: control; Cro: crocin; CS: cigarettes smoke; HMGB1: High mobility group box 1 Glucagon-like peptide-1; RAGE: Receptor for advanced glycation end products.

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AGER/RAGE Antibody - #DF6405