OGG1 Antibody - #DF6401

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Product: OGG1 Antibody
Catalog: DF6401
Description: Rabbit polyclonal antibody to OGG1
Application: WB IHC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Bovine, Sheep, Dog
Mol.Wt.: 39kDa; 39kD(Calculated).
Uniprot: O15527
RRID: AB_2838364

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Bovine(85%), Sheep(85%), Dog(91%)
Clonality:
Polyclonal
Specificity:
OGG1 Antibody detects endogenous levels of total OGG1.
RRID:
AB_2838364
Cite Format: Affinity Biosciences Cat# DF6401, RRID:AB_2838364.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

8 hydroxyguanine DNA glycosylase; 8 oxoguanine DNA glycosylase 1; 8 oxoguanine DNA glycosylase; AP lyase; DNA (apurinic or apyrimidinic site) lyase; DNA apurinic or apyrimidinic site lyase; DNA lyase; DNA-(apurinic or apyrimidinic site) lyase; HMMH; HOGG 1; HOGG1; MMH; MUTM; N glycosylase; Ogg 1; Ogg1; OGG1_HUMAN; OGH 1; OGH1;

Immunogens

Immunogen:

A synthesized peptide derived from human OGG1, corresponding to a region within the internal amino acids.

Uniprot:

O15527(OGG1_HUMAN) >>Visit HPA database.

Gene(ID):
OGG1(4968)
Expression:
O15527 OGG1_HUMAN:

Ubiquitous.

Description:
This gene encodes the enzyme responsible for the excision of 8-oxoguanine, a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen. The action of this enzyme includes lyase activity for chain cleavage. Alternative splicing of the C-terminal region of this gene classifies splice variants into two major groups, type 1 and type 2, depending on the last exon of the sequence. Type 1 alternative splice variants end with exon 7 and type 2 end with exon 8. All variants share the N-terminal region in common, which contains a mitochondrial targeting signal that is essential for mitochondrial localization. Many alternative splice variants for this gene have been described, but the full-length nature for every variant has not been determined. [provided by RefSeq, Aug 2008]
Sequence:
MPARALLPRRMGHRTLASTPALWASIPCPRSELRLDLVLPSGQSFRWREQSPAHWSGVLADQVWTLTQTEEQLHCTVYRGDKSQASRPTPDELEAVRKYFQLDVTLAQLYHHWGSVDSHFQEVAQKFQGVRLLRQDPIECLFSFICSSNNNIARITGMVERLCQAFGPRLIQLDDVTYHGFPSLQALAGPEVEAHLRKLGLGYRARYVSASARAILEEQGGLAWLQQLRESSYEEAHKALCILPGVGTKVADCICLMALDKPQAVPVDVHMWHIAQRDYSWHPTTSQAKGPSPQTNKELGNFFRSLWGPYAGWAQAVLFSADLRQSRHAQEPPAKRRKGSKGPEG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
91
Bovine
85
Sheep
85
Horse
77
Pig
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15527 As Substrate

Site PTM Type Enzyme
S115 Phosphorylation
S183 Phosphorylation
K198 Ubiquitination
S231 Phosphorylation
S232 Phosphorylation
S280 Phosphorylation
K289 Ubiquitination
K297 Ubiquitination
S326 Phosphorylation
K338 Acetylation
K341 Acetylation

Research Backgrounds

Function:

DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion.

Subcellular Location:

Nucleus>Nucleoplasm. Nucleus speckle. Nucleus matrix.
Note: Together with APEX1 is recruited to nuclear speckles in UVA-irradiated cells.

Nucleus.

Mitochondrion.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Family&Domains:

Belongs to the type-1 OGG1 family.

Research Fields

· Genetic Information Processing > Replication and repair > Base excision repair.

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