Claudin 1 Antibody | Affinity Biosciences

Product:	Claudin 1 Antibody
Catalog:	AF0127
Description:	Rabbit polyclonal antibody to Claudin 1
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Chicken
Mol.Wt.:	22kDa; 23kD(Calculated).
Uniprot:	O95832
RRID:	AB_2833311

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(83%)

Clonality:

Polyclonal

Specificity:

Claudin 1 Antibody detects endogenous levels of total Claudin 1.

RRID:

AB_2833311
Cite Format: Affinity Biosciences Cat# AF0127, RRID:AB_2833311.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Claudin-1; Claudin1; CLD 1; CLD1; CLD1_HUMAN; CLDN 1; Cldn1; ILVASC; SEMP 1; SEMP1; Senescence associated epithelial membrane protein 1; Senescence associated epithelial membrane protein; Senescence-associated epithelial membrane protein;

Immunogens

Immunogen:

Uniprot:

O95832(CLD1_HUMAN) >>Visit HPA database.

Gene(ID):

CLDN1(9076)

Expression:

O95832 CLD1_HUMAN:

Strongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis.

Description:

claudin 1 Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity. Acts as a co- receptor for HCV entry into hepatic cells. Belongs to the claudin family. Can form homo- and heteropolymers with other CLDN. Homopolymers interact with CLDN3, but not CLDN2, homopolymers. Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3. Interacts with MPDZ and INADL. May interact with HCV E1 and E2 proteins.

Sequence:

O95832 CLD1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MANAGLQLLGFILAFLGWIGAIVSTALPQWRIYSYAGDNIVTAQAMYEGLWMSCVSQSTGQIQCKVFDSLLNLSSTLQATRALMVVGILLGVIAIFVATVGMKCMKCLEDDEVQKMRMAVIGGAIFLLAGLAILVATAWYGNRIVQEFYDPMTPVNARYEFGQALFTGWAAASLCLLGGALLCCSCPRKTTSYPTPRPYPKPAPSSGKDYV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Rabbit

100

Chicken

Dog

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95832 As Substrate

O95832

Site	PTM Type	Source
K115	Ubiquitination	Uniprot
T153	Phosphorylation	Uniprot
K189	Ubiquitination	Uniprot
T191	O-Glycosylation	Uniprot
T191	Phosphorylation	Uniprot
S192	O-Glycosylation	Uniprot
S192	Phosphorylation	Uniprot
T195	Phosphorylation	Uniprot
K201	Ubiquitination	Uniprot
S205	O-Glycosylation	Uniprot
S205	Phosphorylation	Uniprot
S206	O-Glycosylation	Uniprot
S206	Phosphorylation	Uniprot
K208	Ubiquitination	Uniprot
Y210	Phosphorylation	Uniprot

Research Backgrounds

O95832_CLD1_HUMAN

Function:

Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions.

(Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes. Associates with CD81 and the CLDN1-CD81 receptor complex is essential for HCV entry into host cell. Acts as a receptor for dengue virus.

Subcellular Location:

Cell junction>Tight junction. Cell membrane>Multi-pass membrane protein. Basolateral cell membrane.
Note: Associates with CD81 and the CLDN1-CD81 complex localizes to the basolateral cell membrane.

Tissue Specificity:

Strongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis.

Subunit Structure:

Homopolymers interact with CLDN3, but not CLDN2, homopolymers. Can form homo- and heteropolymers with other claudin family members. Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3. Interacts with MPDZ and PATJ (By similarity). Interacts with OCLN, CLDN4, CLDN6 and CLDN9. Interacts with CD81.

(Microbial infection) Interacts with hepatitis c virus E1 and E2 proteins.

(Microbial infection) Interacts with dengue virus small envelope protein M.

Family&Domains:

Belongs to the claudin family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs). (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)

References

1). Extracellular vesicles of Fusobacterium nucleatum compromise intestinal barrier through targeting RIPK1-mediated cell death pathway. Gut Microbes, 2021 (PubMed: 33769187) [IF=12.2]

Application: IHC Species: mouse Sample: colon

Figure 5. |FnEVs increase gut barrier leakage in experimental colitis models. (a) Representative images and ex vivo imaging with the intestine, liver, heart, spleen and kidney of mice. (b) Relative fluorescence intensity of translocated EGFP-labeled E.coli in every tissues. (c) Representative images of immunohistochemical stainings of ZO-1, claudin-1 and occludin in the colon on day 3 after colitis induction. Scale bar = 50 um.

2). Stem cell membrane-coated isotretinoin for acne treatment. JOURNAL OF NANOBIOTECHNOLOGY, 2020 (PubMed: 32723398) [IF=10.2]

3). Oxyberberine, a novel gut microbiota-mediated metabolite of berberine, possesses superior anti-colitis effect: impact on intestinal epithelial barrier, gut microbiota profile and TLR4-MyD88-NF-κB pathway. PHARMACOLOGICAL RESEARCH, 2020 (PubMed: 31863867) [IF=9.3]

Application: WB Species: Mice Sample: colonic tissues

Fig. 4. OBB protected intestinal epithelial barrier by modulating TJs proteins. Effect of OBB on the mRNA levels of mucin-1(A) and mucin-2 (B). (C) Representative Western blotting images of TJs protein, and the relative protein expressions were normalized to β-actin. (D-H) Changes in the relative protein expression levels of ZO-1, ZO-2, occludin, JAM-A, and claudin-1 were measured respectively. Data are shown as mean ± SEM (n = 3). # P < 0.05, ## P < 0.01 vs. Control group, * P < 0.05, ** P < 0.01 vs. DSS group, & P < 0.05, && P < 0.01 vs. BBR group.

4). Patchouli alcohol attenuates 5-fluorouracil-induced intestinal mucositis via TLR2/MyD88/NF-kB pathway and regulation of microbiota. BIOMEDICINE & PHARMACOTHERAPY, 2020 (PubMed: 32004938) [IF=7.5]

Application: WB Species: rat Sample: Intestinal

Fig. 4. |Effect of PA on intestinal mocusal barrier proteins. (a–d) Relative mRNA expression of MLCK, ZO-1, occludin and claudin-1 (n = 4); (e–i) Expressions of MLC,p-MLC, ZO-1, occludin and claudin-1proteins (n = 3). Values were represented the mean ± SEM. **P < 0.01, *P < 0.05 versus 5-FU group and ##P < 0.01 versus normal group.

5). Allyl isothiocyanate mitigates airway inflammation and constriction in a house dust mite-induced allergic asthma model via upregulation of tight junction proteins and the TRPA1 modulation. Biomedicine & Pharmacotherapy, 2023 (PubMed: 37634475) [IF=7.5]

Application: WB Species: Mouse Sample: lung

Fig. 4. Effects of AITC on tight junction-related protein in lung. AITC treatments on the modulation of tight junction-related protein expression. (A) E-cadherin, (B), ZO-1, (C) Occludin, (D) Claudin-1. Data are expressed in mean ± SEM (n = 5–7). Significant difference presented as different symbol. *p

6). Mesenchymal Stem Cells Overexpressing ACE2 Favorably Ameliorate LPS-Induced Inflammatory Injury in Mammary Epithelial Cells. Frontiers in Immunology, 2022 (PubMed: 35095873) [IF=7.3]

Application: WB Species: Mice Sample: EpH4-Ev cells

Figure 8 MSC-ACE2 upregulates blood-milk barrier-related protein expression to suppress LPS-induced inflammation in Eph4-EV cells. (A) Detection of relative protein expression levels of ZO-1, Claudin-1, Claudin-2 by Western blot; (B) Statistics of the ZO-1, Claudin-1, Claudin-2 Western blot results. Experiments were repeated three times and data were presented as the mean ± SEM (n = 4). * P < 0.05 vs. EpH4-Ev; # P < 0.05 vs. LPS; $ P < 0.05 vs. MSC; & P < 0.05 vs. MSC-GFP.

7). SIRT1 Stabilizes β-TrCP1 to Inhibit Snail1 Expression in Maintaining Intestinal Epithelial Integrity to Alleviate Colitis. Cellular and molecular gastroenterology and hepatology, 2024 (PubMed: 38729522) [IF=7.2]

8). Djulis Hull Improves Insulin Resistance and Modulates the Gut Microbiota in High-Fat Diet (HFD)-Induced Hyperglycaemia. Antioxidants, 2021 (PubMed: 35052549) [IF=7.0]

Application: WB Species: Mice Sample: epididymal white adipose tissue

Figure 7 Effect of djulis hull crude extract on (A) LPS in serum and (B) the protein expression of tight junctions in the colon of high-fat diet-induced hyperglycaemia. ND: normal diet; HFD: high-fat diet; HCE: high dosage of crude extract; LPS: lipopolysaccharide. Values represent the mean ± SEM (n = 6). The statistical methods used one-way ANOVA, and the values with different letters are significantly different at p < 0.05.

9). Palmitoleic Acid on Top of HFD Ameliorates Insulin Resistance Independent of Diacylglycerols and Alters Gut Microbiota in C57BL/6J Mice. Food Science and Human Wellness, 2023 [IF=7.0]

Application: WB Species: Mouse Sample:

Fig. 6 Palmitoleic acid promotes intestinal tight junction integrity and reduces HFD-induced colon inflammation. 6 weeks old mice were fed with HFD for 12 weeks. Mice were treated with palmitoleic acid, oleic acid, or BSA as control via oral gavages for 6 weeks. The colon was collected for further analysis. (A) The level of occludin and claudin-1 by Western blotting. (B) Quantification of occludin level. (C) Quantification of claudin-1 level. (D) Macrophage infiltration by IHC for F4/80. (E) mRNA levels of MCP-1, TNF-α, IL-1β, and IL-6 by RT-PCR. n = 4 mice per group. The data are mean ± SEM (error bars). † P < 0.05, ††P < 0.01, †††P < 0.001, BSA vs palmitoleic acid; ‡ P < 0.05, ‡‡P < 0.01, ‡‡‡P < 0.001, oleic acid vs palmitoleic acid; §§§P < 0.001, BSA vs oleic acid.

10). Lactobacillus paracasei influences the gut-microbiota-targeted metabolic modulation of the immune status of diarrheal mice. Food & function, 2023 (PubMed: 37092300) [IF=6.1]

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Claudin 1 Antibody - #AF0127