Product: Claudin 1 Antibody
Catalog: AF0127
Source: Rabbit
Application: WB, IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Chicken
Mol.Wt.: 22kDa; 23kD(Calculated).
Uniprot: O95832
RRID: AB_2833311

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Product Info

WB 1:500-1:3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(83%)
Claudin 1 Antibody detects endogenous levels of total Claudin 1.
Cite Format: Affinity Biosciences Cat# AF0127, RRID:AB_2833311.
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


Claudin-1; Claudin1; CLD 1; CLD1; CLD1_HUMAN; CLDN 1; Cldn1; ILVASC; SEMP 1; SEMP1; Senescence associated epithelial membrane protein 1; Senescence associated epithelial membrane protein; Senescence-associated epithelial membrane protein;


O95832 CLD1_HUMAN:

Strongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis.

claudin 1 Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity. Acts as a co- receptor for HCV entry into hepatic cells. Belongs to the claudin family. Can form homo- and heteropolymers with other CLDN. Homopolymers interact with CLDN3, but not CLDN2, homopolymers. Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3. Interacts with MPDZ and INADL. May interact with HCV E1 and E2 proteins.



Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95832 As Substrate

Site PTM Type Enzyme
K115 Ubiquitination
T153 Phosphorylation
K189 Ubiquitination
T191 O-Glycosylation
T191 Phosphorylation
S192 O-Glycosylation
S192 Phosphorylation
T195 Phosphorylation
K201 Ubiquitination
S205 O-Glycosylation
S205 Phosphorylation
S206 O-Glycosylation
S206 Phosphorylation
K208 Ubiquitination
Y210 Phosphorylation

Research Backgrounds


Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions.

(Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes. Associates with CD81 and the CLDN1-CD81 receptor complex is essential for HCV entry into host cell. Acts as a receptor for dengue virus.

Subcellular Location:

Cell junction>Tight junction. Cell membrane>Multi-pass membrane protein. Basolateral cell membrane.
Note: Associates with CD81 and the CLDN1-CD81 complex localizes to the basolateral cell membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Strongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis.

Subunit Structure:

Homopolymers interact with CLDN3, but not CLDN2, homopolymers. Can form homo- and heteropolymers with other claudin family members. Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3. Interacts with MPDZ and PATJ (By similarity). Interacts with OCLN, CLDN4, CLDN6 and CLDN9. Interacts with CD81.

(Microbial infection) Interacts with hepatitis c virus E1 and E2 proteins.

(Microbial infection) Interacts with dengue virus small envelope protein M.


Belongs to the claudin family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)


1). Li C et al. Oxyberberine, a novel gut microbiota-mediated metabolite of berberine, possesses superior anti-colitis effect: Impact on intestinal epithelial barrier, gut microbiota profile and TLR4-MyD88-NF-κB pathway. Pharmacol Res 2020 Feb;152:104603 (PubMed: 31863867) [IF=10.334]

Application: WB    Species: Mice    Sample: colonic tissues

Fig. 4. OBB protected intestinal epithelial barrier by modulating TJs proteins. Effect of OBB on the mRNA levels of mucin-1(A) and mucin-2 (B). (C) Representative Western blotting images of TJs protein, and the relative protein expressions were normalized to β-actin. (D-H) Changes in the relative protein expression levels of ZO-1, ZO-2, occludin, JAM-A, and claudin-1 were measured respectively. Data are shown as mean ± SEM (n = 3). # P < 0.05, ## P < 0.01 vs. Control group, * P < 0.05, ** P < 0.01 vs. DSS group, & P < 0.05, && P < 0.01 vs. BBR group.

2). Liu L et al. Extracellular vesicles of Fusobacterium nucleatum compromise intestinal barrier through targeting RIPK1-mediated cell death pathway. Gut Microbes 2021;13(1):1-20. (PubMed: 33769187) [IF=9.434]

Application: IHC    Species: mouse    Sample: colon

Figure 5. |FnEVs increase gut barrier leakage in experimental colitis models. (a) Representative images and ex vivo imaging with the intestine, liver, heart, spleen and kidney of mice. (b) Relative fluorescence intensity of translocated EGFP-labeled E.coli in every tissues. (c) Representative images of immunohistochemical stainings of ZO-1, claudin-1 and occludin in the colon on day 3 after colitis induction. Scale bar = 50 um.

3). Yan S et al. Mesenchymal Stem Cells Overexpressing ACE2 Favorably Ameliorate LPS-Induced Inflammatory Injury in Mammary Epithelial Cells. Front Immunol 2022 Jan 14;12:796744. (PubMed: 35095873) [IF=8.786]

Application: WB    Species: Mice    Sample: EpH4-Ev cells

Figure 8 MSC-ACE2 upregulates blood-milk barrier-related protein expression to suppress LPS-induced inflammation in Eph4-EV cells. (A) Detection of relative protein expression levels of ZO-1, Claudin-1, Claudin-2 by Western blot; (B) Statistics of the ZO-1, Claudin-1, Claudin-2 Western blot results. Experiments were repeated three times and data were presented as the mean ± SEM (n = 4). * P < 0.05 vs. EpH4-Ev; # P < 0.05 vs. LPS; $ P < 0.05 vs. MSC; & P < 0.05 vs. MSC-GFP.

4). Wu J et al. Patchouli alcohol attenuates 5-fluorouracil-induced intestinal mucositis via TLR2/MyD88/NF-kB pathway and regulation of microbiota. Biomed Pharmacother 2020 Jan 28;124:109883 (PubMed: 32004938) [IF=7.419]

Application: WB    Species: rat    Sample: Intestinal

Fig. 4. |Effect of PA on intestinal mocusal barrier proteins. (a–d) Relative mRNA expression of MLCK, ZO-1, occludin and claudin-1 (n = 4); (e–i) Expressions of MLC,p-MLC, ZO-1, occludin and claudin-1proteins (n = 3). Values were represented the mean ± SEM. **P < 0.01, *P < 0.05 versus 5-FU group and ##P < 0.01 versus normal group.

5). Zhang G et al. Assessment of the effect of ethanol extracts from Cinnamomum camphora seed kernel on intestinal inflammation using simulated gastrointestinal digestion and a Caco-2/RAW264.7 co-culture system. Food Funct 2021 Oct 4;12(19):9197-9210. (PubMed: 34606534) [IF=6.317]

6). Tung YT et al. Djulis Hull Improves Insulin Resistance and Modulates the Gut Microbiota in High-Fat Diet (HFD)-Induced Hyperglycaemia. Antioxidants (Basel) 2021 Dec 26;11(1):45. (PubMed: 35052549) [IF=6.312]

Application: WB    Species: Mice    Sample: epididymal white adipose tissue

Figure 7 Effect of djulis hull crude extract on (A) LPS in serum and (B) the protein expression of tight junctions in the colon of high-fat diet-induced hyperglycaemia. ND: normal diet; HFD: high-fat diet; HCE: high dosage of crude extract; LPS: lipopolysaccharide. Values represent the mean ± SEM (n = 6). The statistical methods used one-way ANOVA, and the values with different letters are significantly different at p < 0.05.

7). Huang S et al. Lipopolysaccharide induced intestinal epithelial injury: a novel organoids-based model for sepsis in vitro. Chin Med J (Engl) 2022 Oct 24. (PubMed: 36355867) [IF=6.133]

Application: IF/ICC    Species: Mouse    Sample:

Figure 2 The immunofluorescence of organoids with or without LPS stimulation. The images were captured by Opera Phenix™ high-content imaging system and compressed by Harmony. (A) The expression of Ki-67, LPS 100 μg/mL, 24 h. (B) The expression of ZO-1, LPS 100 μg/mL, 24 h. (C) The expression of occludins, LPS 100 μg/mL, 24 h. (D) The expression of claudin-1, LPS 100 μg/mL, 24 h. (E) Quantitative analysis of Ki-67 with different concentrations of LPS stimulated for 8 h or 24 h. (F) Quantitative analysis of ZO-1 with different concentrations of LPS stimulated for 8 h or 24 h. (G) Quantitative analysis of occludins with different concentrations of LPS stimulated for 8 h or 24 h. (H) Quantitative analysis of claudin-1 with different concentrations of LPS stimulated for 8 h or 24 h. Data are presented as means ± SD, compared with LPS 0 μg/mL 8 h or 24 h group accordingly using Student's t-tests. ∗ is used for 24 h. SD: Standard deviation; LPS: Lipopolysaccharide; ZO-1: Zonula occludens-1.

8). Huang Z et al. Industrially Produced Rice Protein Ameliorates Dextran Sulfate Sodium-Induced Colitis via Protecting the Intestinal Barrier, Mitigating Oxidative Stress, and Regulating Gut Microbiota. J Agric Food Chem 2022 Apr 27;70(16):4952-4965. (PubMed: 35412826) [IF=5.895]

9). Xu Q et al. Propionate Ameliorates Alcohol-Induced Liver Injury in Mice via the Gut–Liver Axis: Focus on the Improvement of Intestinal Permeability. J Agric Food Chem 2022 May 12. (PubMed: 35549256) [IF=5.895]

10). Yao H et al. Matrine protects against DSS-induced murine colitis by improving gut barrier integrity, inhibiting the PPAR-α signaling pathway, and modulating gut microbiota. Int Immunopharmacol 2021 Nov;100:108091. (PubMed: 34474274) [IF=5.714]

Application: WB    Species: mouse    Sample: colon

Fig. 4. |Matrine improved gut barrier integrity in DSS-induced UC mice.(C) The expressions of occludin, claudin-1,2, and ZO1 protein were verified by western blot.

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