JAM1 Antibody - #DF6373

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Product: JAM1 Antibody
Catalog: DF6373
Description: Rabbit polyclonal antibody to JAM1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Horse, Sheep, Rabbit, Dog
Mol.Wt.: 33kDa; 33kD(Calculated).
Uniprot: Q9Y624
RRID: AB_2838337

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Horse(82%), Sheep(91%), Rabbit(82%), Dog(82%)
Clonality:
Polyclonal
Specificity:
JAM1 Antibody detects endogenous levels of total JAM1.
RRID:
AB_2838337
Cite Format: Affinity Biosciences Cat# DF6373, RRID:AB_2838337.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CD 321; CD321; CD321 antigen; ESTM33; F11 receptor; F11R; JAM 1; JAM A; JAM; JAM-1; JAM-A; JAM1; JAM1_HUMAN; JAMA; JCAM; Jcam1; Junction adhesion molecule 1; Junction adhesion molecule, mouse, homolog of; Junctional adhesion molecule 1; Junctional adhesion molecule A; KAT; Ly106; PAM 1; PAM-1; PAM1; Platelet adhesion molecule 1; Platelet adhesion molecule; Platelet F11 receptor; PRO301; UNQ264;

Immunogens

Immunogen:
Uniprot:

Q9Y624(JAM1_HUMAN) >>Visit HPA database.

Gene(ID):
F11R(50848)
Expression:
Q9Y624 JAM1_HUMAN:

Expressed in endothelium, epithelium and leukocytes (at protein level).

Description:
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as (1) a receptor for reovirus, (2) a ligand for the integrin LFA1, involved in leukocyte transmigration, and (3) a platelet receptor. Multiple 5' alternatively spliced variants, encoding the same protein, have been identified but their biological validity has not been established. [provided by RefSeq, Jul 2008]
Sequence:
MGTKAQVERKLLCLFILAILLCSLALGSVTVHSSEPEVRIPENNPVKLSCAYSGFSSPRVEWKFDQGDTTRLVCYNNKITASYEDRVTFLPTGITFKSVTREDTGTYTCMVSEEGGNSYGEVKVKLIVLVPPSKPTVNIPSSATIGNRAVLTCSEQDGSPPSEYTWFKDGIVMPTNPKSTRAFSNSSYVLNPTTGELVFDPLSASDTGEYSCEARNGYGTPMTSNAVRMEAVERNVGVIVAAVLVTLILLGILVFGIWFAYSRGHFDRTKKGTSSKKVIYSQPSARSEGEFKQTSSFLV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Sheep
91
Horse
82
Dog
82
Rabbit
82
Bovine
73
Chicken
73
Xenopus
44
Pig
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y624 As Substrate

Site PTM Type Enzyme
R86 Methylation
K97 Ubiquitination
S98 Phosphorylation
T100 Phosphorylation
S133 Phosphorylation
T175 O-Glycosylation
K178 Ubiquitination
N185 N-Glycosylation
S262 Phosphorylation
T273 Phosphorylation
S275 Phosphorylation
K277 Ubiquitination
Y280 Phosphorylation
S281 Phosphorylation
S284 Phosphorylation P17252 (PRKCA)
S287 Phosphorylation
K292 Ubiquitination
S295 Phosphorylation
S296 Phosphorylation

Research Backgrounds

Function:

Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier (By similarity). Ligand for integrin alpha-L/beta-2 involved in memory T-cell and neutrophil transmigration. Involved in platelet activation.

(Microbial infection) Acts as a receptor for Mammalian reovirus sigma-1.

(Microbial infection) Acts as a receptor for Human Rotavirus strain Wa.

PTMs:

N-glycosylated.

Subcellular Location:

Cell junction>Tight junction. Cell membrane>Single-pass type I membrane protein.
Note: Localized at tight junctions of both epithelial and endothelial cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in endothelium, epithelium and leukocytes (at protein level).

Subunit Structure:

Interacts with the ninth PDZ domain of MPDZ. Interacts with the first PDZ domain of PARD3. The association between PARD3 and PARD6B probably disrupts this interaction (By similarity). Interacts with ITGAL (via I-domain).

(Microbial infection) Interacts with Mammalian reovirus sigma-1 capsid protein.

(Microbial infection) Interacts with Human Rotavirus strain Wa vp4 capsid protein.

Family&Domains:

The Ig-like V-type 2 domain is necessary and sufficient for interaction with integrin alpha-L/beta-2.

Belongs to the immunoglobulin superfamily.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

References

1). Oxyberberine, a novel gut microbiota-mediated metabolite of berberine, possesses superior anti-colitis effect: impact on intestinal epithelial barrier, gut microbiota profile and TLR4-MyD88-NF-κB pathway. PHARMACOLOGICAL RESEARCH, 2020 (PubMed: 31863867) [IF=9.3]

Application: WB    Species: Mice    Sample: colonic tissues

Fig. 4. OBB protected intestinal epithelial barrier by modulating TJs proteins. Effect of OBB on the mRNA levels of mucin-1(A) and mucin-2 (B). (C) Representative Western blotting images of TJs protein, and the relative protein expressions were normalized to β-actin. (D-H) Changes in the relative protein expression levels of ZO-1, ZO-2, occludin, JAM-A, and claudin-1 were measured respectively. Data are shown as mean ± SEM (n = 3). # P < 0.05, ## P < 0.01 vs. Control group, * P < 0.05, ** P < 0.01 vs. DSS group, & P < 0.05, && P < 0.01 vs. BBR group.
2). The reduced SCFA-producing gut microbes are involved in the inflammatory activation in Kawasaki disease. Frontiers in Immunology, 2023 (PubMed: 37398673) [IF=7.3]

Application: IF/ICC    Species: Mouse    Sample:

Figure 3 Gut dysbiosis contributed to the leaky gut in the KD mouse model. (A, B) The expression of intestinal barrier proteins Claudin-1, Jam-1, Occludin and ZO-1 was assessed by qPCR and western blot when using C.butyricum or antibiotics. (C) Immunofluorescence staining showed different expressions of intestinal barrier proteins among groups. Magnification: × 200. Scale bars = 100 µm. (D) The concentration of plasma D-Lac was detected in PBS group (n=3), CAWS group (n=7), CAWS+C. butyricum group (n=6) and CAWS+ABX group (n=3). Values are expressed as the mean ± SEM. Significance: ** P < 0.01, *** P < 0.001, vs. the PBS group; # P < 0.05, ## P < 0.01, ### P < 0.01 vs. the CAWS group; ns means no significance.

Application: WB    Species: Mouse    Sample:

Figure 3 Gut dysbiosis contributed to the leaky gut in the KD mouse model. (A, B) The expression of intestinal barrier proteins Claudin-1, Jam-1, Occludin and ZO-1 was assessed by qPCR and western blot when using C.butyricum or antibiotics. (C) Immunofluorescence staining showed different expressions of intestinal barrier proteins among groups. Magnification: × 200. Scale bars = 100 µm. (D) The concentration of plasma D-Lac was detected in PBS group (n=3), CAWS group (n=7), CAWS+C. butyricum group (n=6) and CAWS+ABX group (n=3). Values are expressed as the mean ± SEM. Significance: ** P < 0.01, *** P < 0.001, vs. the PBS group; # P < 0.05, ## P < 0.01, ### P < 0.01 vs. the CAWS group; ns means no significance.

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