ERG Antibody - #DF6372

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Product: ERG Antibody
Catalog: DF6372
Description: Rabbit polyclonal antibody to ERG
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 55kDa; 55kD(Calculated).
Uniprot: P11308
RRID: AB_2838336

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 100ul $280 In stock
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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(90%), Horse(90%), Sheep(80%), Dog(90%), Chicken(80%), Xenopus(89%)
Clonality:
Polyclonal
Specificity:
ERG Antibody detects endogenous levels of total ERG.
RRID:
AB_2838336
Cite Format: Affinity Biosciences Cat# DF6372, RRID:AB_2838336.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Avian erythroblastosis virus E-26 (v-ets) oncogene related; D030036I24Rik; Erg 3; Erg; ERG/EWS fusion gene, included; ERG/FUS fusion gene, included; ERG/TMPSSR2 fusion gene, included; ERG_HUMAN; ERG1, included; ERG2, included; ets related; ETS-related gene; KCNH2; Oncogene ERG; p55; TMPRSS2/ERG fusion; transcriptional regulator ERG (transforming protein ERG); Transcriptional regulator ERG; Transforming protein ERG; v ets avian erythroblastosis virus E26 oncogene; v ets avian erythroblastosis virus E26 oncogene related; v ets erythroblastosis virus E26 oncogene homolog; v ets erythroblastosis virus E26 oncogene like; v ets erythroblastosis virus E26 oncogene like isoform 2; v-ets erythroblastosis virus E26 oncogene; v-ets erythroblastosis virus E26 oncogene homolog (avian); V-ets erythroblastosis virus E26 oncogene like (Avian), isoform CRA_e;

Immunogens

Immunogen:
Uniprot:

P11308(ERG_HUMAN) >>Visit HPA database.

Gene(ID):
ERG(2078)
Description:
This gene encodes a member of the erythroblast transformation-specific (ETS) family of transcriptions factors. All members of this family are key regulators of embryonic development, cell proliferation, differentiation, angiogenesis, inflammation, and apoptosis. The protein encoded by this gene is mainly expressed in the nucleus. It contains an ETS DNA-binding domain and a PNT (pointed) domain which is implicated in the self-association of chimeric oncoproteins. This protein is required for platelet adhesion to the subendothelium, inducing vascular cell remodeling. It also regulates hematopoesis, and the differentiation and maturation of megakaryocytic cells. This gene is involved in chromosomal translocations, resulting in different fusion gene products, such as TMPSSR2-ERG and NDRG1-ERG in prostate cancer, EWS-ERG in Ewing's sarcoma and FUS-ERG in acute myeloid leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jan 2012]
Sequence:
MIQTVPDPAAHIKEALSVVSEDQSLFECAYGTPHLAKTEMTASSSSDYGQTSKMSPRVPQQDWLSQPPARVTIKMECNPSQVNGSRNSPDECSVAKGGKMVGSPDTVGMNYGSYMEEKHMPPPNMTTNERRVIVPADPTLWSTDHVRQWLEWAVKEYGLPDVNILLFQNIDGKELCKMTKDDFQRLTPSYNADILLSHLHYLRETPLPHLTSDDVDKALQNSPRLMHARNTGGAAFIFPNTSVYPEATQRITTRPDLPYEPPRRSAWTGHGHPTPQSKAAQPSPSTVPKTEDQRPQLDPYQILGPTSSRLANPGSGQIQLWQFLLELLSDSSNSSCITWEGTNGEFKMTDPDEVARRWGERKSKPNMNYDKLSRALRYYYDKNIMTKVHGKRYAYKFDFHGIAQALQPHPPESSLYKYPSDLPYMGSYHAHPQKMNFVAPHPPALPVTSSSFFAAPNPYWNSPTGGIYPNTRLPTSHMPSHLGTYY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
90
Horse
90
Dog
90
Xenopus
89
Sheep
80
Chicken
80
Bovine
70
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P11308 As Substrate

Site PTM Type Enzyme
T4 Phosphorylation
T32 Phosphorylation
S44 Phosphorylation P48729 (CSNK1A1)
S45 Phosphorylation P48729 (CSNK1A1)
S46 Phosphorylation P48729 (CSNK1A1)
Y48 Phosphorylation
S52 Phosphorylation
S55 Phosphorylation
S85 Phosphorylation
S88 Phosphorylation
S93 Phosphorylation
K96 Acetylation
K99 Acetylation
S103 Phosphorylation
S222 Phosphorylation P28482 (MAPK1)
Y259 Phosphorylation
S283 Phosphorylation P28482 (MAPK1)
S285 Phosphorylation
Y300 Phosphorylation
K362 Ubiquitination
Y369 Phosphorylation
K371 Ubiquitination
K387 Ubiquitination
S413 Phosphorylation

Research Backgrounds

Function:

Transcriptional regulator. May participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure.

Subcellular Location:

Nucleus. Cytoplasm.
Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Interacts with SETDB1.

Family&Domains:

Belongs to the ETS family.

Research Fields

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

Restrictive clause

 

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For Research Use Only.
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