PLA2G2A Antibody | Affinity Biosciences

WB
IF/ICC
Cites

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Western blot analysis of extracts from various samples, using PLA2G2A Antibody.

Product:	PLA2G2A Antibody
Catalog:	DF6366
Description:	Rabbit polyclonal antibody to PLA2G2A
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Horse, Rabbit
Mol.Wt.:	16kDa; 16kD(Calculated).
Uniprot:	P14555
RRID:	AB_2838330

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(80%), Horse(90%), Rabbit(80%)

Clonality:

Polyclonal

Specificity:

PLA2G2A Antibody detects endogenous levels of total PLA2G2A.

RRID:

AB_2838330
Cite Format: Affinity Biosciences Cat# DF6366, RRID:AB_2838330.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

EC 3.1.1.4; GIIC sPLA2; Group IIA phospholipase A2; membrane associated; MOM1; Non pancreatic secretory phospholipase A2; Non-pancreatic secretory phospholipase A2; NPS PLA2; NPS-PLA2; NPSPLA2; PA2GA_HUMAN; Phosphatidylcholine 2 acylhydrolase; Phosphatidylcholine 2-acylhydrolase 2A; Phospholipase A2; Phospholipase A2 group IIA (platelets synovial fluid); Phospholipase A2 membrane associated precursor; PLA2; PLA2B; PLA2G2A; PLA2L; PLA2S; PLAS1; RASF A; RASFA; sPLA2;

Immunogens

Immunogen:

Uniprot:

P14555(PA2GA_HUMAN) >>Visit HPA database.

Gene(ID):

PLA2G2A(5320)

Description:

The protein encoded by this gene is a member of the phospholipase A2 family (PLA2). PLA2s constitute a diverse family of enzymes with respect to sequence, function, localization, and divalent cation requirements. This gene product belongs to group II, which contains secreted form of PLA2, an extracellular enzyme that has a low molecular mass and requires calcium ions for catalysis. It catalyzes the hydrolysis of the sn-2 fatty acid acyl ester bond of phosphoglycerides, releasing free fatty acids and lysophospholipids, and thought to participate in the regulation of the phospholipid metabolism in biomembranes. Several alternatively spliced transcript variants with different 5' UTRs have been found for this gene.[provided by RefSeq, Sep 2009]

Sequence:

P14555 PA2GA_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKTLLLLAVIMIFGLLQAHGNLVNFHRMIKLTTGKEAALSYGFYGCHCGVGGRGSPKDATDRCCVTHDCCYKRLEKRGCGTKFLSYKFSNSGSRITCAKQDSCRSQLCECDKAAATCFARNKTTYNKKYQYYSNKHCRGSTPRC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

Pig

Rabbit

Bovine

Sheep

Dog

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P14555 As Substrate

P14555

Site	PTM Type	Source
K82	Ubiquitination	Uniprot
Y86	Phosphorylation	Uniprot
K128	Sumoylation	Uniprot

Research Backgrounds

P14555_PA2GA_HUMAN

Function:

Catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Thought to participate in the regulation of phospholipid metabolism in biomembranes including eicosanoid biosynthesis. Independent of its catalytic activity, acts as a ligand for integrins. Binds to and activates integrins ITGAV:ITGB3, ITGA4:ITGB1 and ITGA5:ITGB1. Binds to a site (site 2) which is distinct from the classical ligand-binding site (site 1) and induces integrin conformational changes and enhanced ligand binding to site 1. Induces cell proliferation in an integrin-dependent manner.

Subcellular Location:

Cell membrane>Peripheral membrane protein. Secreted.

Family&Domains:

Belongs to the phospholipase A2 family.

Research Fields

· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)

· Metabolism > Lipid metabolism > Glycerophospholipid metabolism.

· Metabolism > Lipid metabolism > Ether lipid metabolism.

· Metabolism > Lipid metabolism > Arachidonic acid metabolism.

· Metabolism > Lipid metabolism > Linoleic acid metabolism.

· Metabolism > Lipid metabolism > alpha-Linolenic acid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction. (View pathway)

· Organismal Systems > Digestive system > Pancreatic secretion.

· Organismal Systems > Digestive system > Fat digestion and absorption.

References

1). Phospholipase A2 regulates autophagy in gouty arthritis: proteomic and metabolomic studies. Journal of Translational Medicine (PubMed: 37069596) [IF=7.4]

Application: WB Species: Human Sample:

Fig. 6 Interactions between the proteome and metabolome, and validation of the autophagy pathway. A: Example diagram of protein and metabolism KGML network. Squares represent pathways, triangles represent proteins, and circles represent metabolites. Red indicates up-regulated proteins or green indicates down-regulated proteins or metabolites. B: The expressions of LC3B and SQSTM1/62 were evaluated by western blotting. C: The expressions of PLA2 were evaluated by western blotting. D-F: Quantification of LC3B, SQSTM1/62 and PLA2 expressions (n = 3, all the data are expressed as means ± SD, two-way ANOVA followed by Turkey's post hoc test was applied)

2). Identification and immunological characterization of PLA2G2A and cell death-associated molecular clusters in idiopathic pulmonary fibrosis. Life Sciences (PubMed: 37673297) [IF=6.1]

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PLA2G2A Antibody - #DF6366