IL7R Antibody | Affinity Biosciences

Cites

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Figure 6 Circ_MTM1 targeted miR-122-5p to regulate IL7R expression.

Product:	IL7R Antibody
Catalog:	DF6362
Description:	Rabbit polyclonal antibody to IL7R
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	52~80kDa; 52kD(Calculated).
Uniprot:	P16871
RRID:	AB_2838326

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

IL7R Antibody detects endogenous levels of total IL7R.

RRID:

AB_2838326
Cite Format: Affinity Biosciences Cat# DF6362, RRID:AB_2838326.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CD 127; CD127; CD127 antigen; CDw127; IL 7R alpha; IL 7R; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7R-alpha; IL-7RA; IL7R; IL7RA; IL7RA_HUMAN; IL7Ralpha; ILRA; Interleukin 7 receptor alpha chain; Interleukin 7 receptor; Interleukin 7 receptor isoform H5 6; Interleukin-7 receptor subunit alpha;

Immunogens

Immunogen:

A synthesized peptide derived from human IL7R, corresponding to a region within N-terminal amino acids.

Uniprot:

P16871(IL7RA_HUMAN) >>Visit HPA database.

Gene(ID):

IL7R(3575)

Description:

The protein encoded by this gene is a receptor for interleukine 7 (IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukine 2, 4, 7, 9, and 15. This protein has been shown to play a critical role in the V(D)J recombination during lymphocyte development. This protein is also found to control the accessibility of the TCR gamma locus by STAT5 and histone acetylation. Knockout studies in mice suggested that blocking apoptosis is an essential function of this protein during differentiation and activation of T lymphocytes. The functional defects in this protein may be associated with the pathogenesis of the severe combined immunodeficiency (SCID). [provided by RefSeq, Jul 2008]

Sequence:

P16871 IL7RA_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MTILGTTFGMVFSLLQVVSGESGYAQNGDLEDAELDDYSFSCYSQLEVNGSQHSLTCAFEDPDVNITNLEFEICGALVEVKCLNFRKLQEIYFIETKKFLLIGKSNICVKVGEKSLTCKKIDLTTIVKPEAPFDLSVVYREGANDFVVTFNTSHLQKKYVKVLMHDVAYRQEKDENKWTHVNLSSTKLTLLQRKLQPAAMYEIKVRSIPDHYFKGFWSEWSPSYYFRTPEINNSSGEMDPILLTISILSFFSVALLVILACVLWKKRIKPIVWPSLPDHKKTLEHLCKKPRKNLNVSFNPESFLDCQIHRVDDIQARDEVEGFLQDTFPQQLEESEKQRLGGDVQSPNCPSEDVVITPESFGRDSSLTCLAGNVSACDAPILSSSRSLDCRESGKNGPHVYQDLLLSLGTTNSTLPPPFSLQSGILTLNPVAQGQPILTSLGSNQEEAYVTMSSFYQNQ

PTMs - P16871 As Substrate

P16871

Site	PTM Type	Source
N49	N-Glycosylation	Uniprot
N65	N-Glycosylation	Uniprot
N151	N-Glycosylation	Uniprot
Y159	Phosphorylation	Uniprot
Y169	Phosphorylation	Uniprot
K292	Ubiquitination	Uniprot
S297	Phosphorylation	Uniprot
K337	Ubiquitination	Uniprot
S346	Phosphorylation	Uniprot
S365	Phosphorylation	Uniprot
S375	Phosphorylation	Uniprot
Y449	Phosphorylation	Uniprot

Research Backgrounds

P16871_IL7RA_HUMAN

Function:

Receptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP).

PTMs:

N-glycosylated IL-7Ralpha binds IL7 300-fold more tightly than the unglycosylated form.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Cell membrane>Single-pass type I membrane protein.

Secreted.

Subunit Structure:

The IL7 receptor is a heterodimer of IL7R and IL2RG. The TSLP receptor is a heterodimer of CRLF2 and IL7R.

Family&Domains:

The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.

The box 1 motif is required for JAK interaction and/or activation.

Belongs to the type I cytokine receptor family. Type 4 subfamily.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction. (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Immune diseases > Primary immunodeficiency.

· Organismal Systems > Immune system > Hematopoietic cell lineage. (View pathway)

References

1). Circ_MTM1 knockdown inhibits the progression of HBV-related liver fibrosis via regulating IL7R expression through targeting miR-122-5p. American Journal of Translational Research, 2022 (PubMed: 35559382) [IF=2.2]

Application: WB Species: Human Sample: LX-2 cells

Figure 6 Circ_MTM1 targeted miR-122-5p to regulate IL7R expression. HBV-infected LX-2 cells and LX-2 cells with HBV and TGF-β1 co-treatment were transfected with si-NC, si-circ_MTM1, si-circ_MTM1 + anti-miR-NC, or si-circ_MTM1 + anti-miR-122-5p, respectively. A-D. IL7R expression was detected in HBV-infected LX-2 cells and LX-2 cells with HBV and TGF-β1 co-treatment. A-D. *P < 0.05, si-circ_MTM1 group compared with si-NC group; si-circ_MTM1 + anti-miR-122-5p group compared with si-circ_MTM1 + anti-miR-NC group, n = 6. Intergroup comparisons were analyzed by one-way ANOVA with post hoc Bonferroni test, and the intragroup differences were analyzed by Student’s t-test.

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IL7R Antibody - #DF6362