CST8 Antibody | Affinity Biosciences

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Western blot analysis of HEK293 cell lysates, using CST8 Antibody.

DF6356 at 1/100 staining Human pancreatic cancer and adjacent nomal tissues by IHC-P.

DF6356 at 1/100 staining Mouse liver tissue by IHC-P.

Product:	CST8 Antibody
Catalog:	DF6356
Description:	Rabbit polyclonal antibody to CST8
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	16kDa; 16kD(Calculated).
Uniprot:	O60676
RRID:	AB_2838320

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

CST8 Antibody detects endogenous levels of total CST8.

RRID:

AB_2838320
Cite Format: Affinity Biosciences Cat# DF6356, RRID:AB_2838320.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CRES; CST 8; Cst8; CST8_HUMAN; cystatin 8 (cystatin related epididymal specific); Cystatin 8; Cystatin related epididymal specific; Cystatin related epididymal specific protein; Cystatin related epididymal spermatogenic protein; Cystatin related protein epididymis specific; Cystatin-8; Cystatin-related epididymal spermatogenic protein;

Immunogens

Immunogen:

Uniprot:

O60676(CST8_HUMAN) >>Visit HPA database.

Gene(ID):

CST8(10047)

Expression:

O60676 CST8_HUMAN:

Proximal caput region of the epididymis. Lower expression in the testis. Within the testis it is localized to the elongating spermatids, whereas within the epididymis it is exclusively synthesized by the proximal caput epithelium.

Description:

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a protein similar to type 2 cystatins. The protein exhibits highly tissue-specific expression in the reproductive tract, suggesting implicit roles in reproduction. Alternative splicing identified in mouse is suggested in human based on EST evidence but the full-length nature of putative variants has not been determined. [provided by RefSeq, Jul 2008]

Sequence:

O60676 CST8_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPRCRWLSLILLTIPLALVARKDPKKNETGVLRKLKPVNASNANVKQCLWFAMQEYNKESEDKYVFLVVKTLQAQLQVTNLLEYLIDVEIARSDCRKPLSTNEICAIQENSKLKRKLSCSFLVGALPWNGEFTVMEKKCEDA

PTMs - O60676 As Substrate

O60676

Site	PTM Type	Source
N39	N-Glycosylation	Uniprot
K97	Acetylation	Uniprot
K114	Acetylation	Uniprot
K116	Acetylation	Uniprot

Research Backgrounds

O60676_CST8_HUMAN

Function:

Performs a specialized role during sperm development and maturation.

Subcellular Location:

Secreted.

Tissue Specificity:

Family&Domains:

Belongs to the cystatin family.

Restrictive clause

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For Research Use Only.
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CST8 Antibody - #DF6356