ITPA Antibody - #DF6353

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Product: ITPA Antibody
Catalog: DF6353
Description: Rabbit polyclonal antibody to ITPA
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 21kDa; 21kD(Calculated).
Uniprot: Q9BY32
RRID: AB_2838317

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(82%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(91%), Xenopus(82%)
Clonality:
Polyclonal
Specificity:
ITPA Antibody detects endogenous levels of total ITPA.
RRID:
AB_2838317
Cite Format: Affinity Biosciences Cat# DF6353, RRID:AB_2838317.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C20orf37; dJ794I6.3; HLC14-06-P; Inosine triphosphatase (nucleoside triphosphate pyrophosphatase); Inosine triphosphatase; inosine triphosphatase-A; Inosine triphosphate pyrophosphatase; Inosine triphosphate pyrophosphohydrolase; Itpa; ITPA_HUMAN; ITPase; My049; My049 protein; Non canonical purine NTP pyrophosphatase; Non standard purine NTP pyrophosphatase; NTPase; nucleoside triphosphate diphosphatase; Nucleoside triphosphate pyrophosphatase; OK/SW-cl.9; OTTHUMP00000030094; OTTHUMP00000160459; Putative oncogene protein hlc14-06-p;

Immunogens

Immunogen:
Uniprot:

Q9BY32(ITPA_HUMAN) >>Visit HPA database.

Gene(ID):
ITPA(3704)
Expression:
Q9BY32 ITPA_HUMAN:

Ubiquitous. Highly expressed in heart, liver, sex glands, thyroid and adrenal gland.

Description:
ITPA(Inosine triphosphate pyrophosphatase) catalyzes the pyrophosphohydrolysis of inosine triphosphate (ITP) to inosine monophosphate (IMP).It expresses in the cytoplasm(PMID:11278832).It can be detected a band of 44 Kda as a homodimer in the western blot(PMID:19631656) though its predictedsize is 21kd.This protein has two isforms with the molecular weight of 20KDa and 21KDa.This antibody is specific to this protein.
Sequence:
MAASLVGKKIVFVTGNAKKLEEVVQILGDKFPCTLVAQKIDLPEYQGEPDEISIQKCQEAVRQVQGPVLVEDTCLCFNALGGLPGPYIKWFLEKLKPEGLHQLLAGFEDKSAYALCTFALSTGDPSQPVRLFRGRTSGRIVAPRGCQDFGWDPCFQPDGYEQTYAEMPKAEKNAVSHRFRALLELQEYFGSLAA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
91
Xenopus
82
Zebrafish
82
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BY32 As Substrate

Site PTM Type Enzyme
S4 Phosphorylation
K9 Ubiquitination
T14 Phosphorylation
K18 Ubiquitination
K30 Ubiquitination
K39 Ubiquitination
K56 Ubiquitination
K96 Ubiquitination
S111 Phosphorylation
Y113 Phosphorylation
C146 S-Nitrosylation
C154 S-Nitrosylation
K169 Ubiquitination
K172 Ubiquitination

Research Backgrounds

Function:

Pyrophosphatase that hydrolyzes the non-canonical purine nucleotides inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) as well as 2'-deoxy-N-6-hydroxylaminopurine triposphate (dHAPTP) and xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous. Highly expressed in heart, liver, sex glands, thyroid and adrenal gland.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the HAM1 NTPase family.

Research Fields

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

 

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