STIP1 Antibody | Affinity Biosciences

1/1

Western blot analysis of extracts from mouse brain and HepG2, using STIP1 Antibody.

Product:	STIP1 Antibody
Catalog:	DF6351
Description:	Rabbit polyclonal antibody to STIP1
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	63kDa; 63kD(Calculated).
Uniprot:	P31948
RRID:	AB_2838315

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(92%), Bovine(92%), Horse(92%), Sheep(92%), Rabbit(83%), Dog(92%)

Clonality:

Polyclonal

Specificity:

STIP1 Antibody detects endogenous levels of total STIP1.

RRID:

AB_2838315
Cite Format: Affinity Biosciences Cat# DF6351, RRID:AB_2838315.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Epididymis secretory sperm binding protein Li 94n; HEL S 94n; Hop; Hsc70/Hsp90 organizing protein; Hsc70/Hsp90-organizing protein; IEF SSP 3521; NY REN 11 antigen; P60; Renal carcinoma antigen NY-REN-11; STI1; STI1L; STIP1; STIP1_HUMAN; Stress induced phosphoprotein 1; Stress-induced-phosphoprotein 1; Transformation sensitive protein IEF SSP 3521; Transformation-sensitive protein IEF SSP 3521;

Immunogens

Immunogen:

Uniprot:

P31948(STIP1_HUMAN) >>Visit HPA database.

Gene(ID):

STIP1(10963)

Description:

HOP, also known as stress-induced phospho protein 1 (STIP), is a co-chaperone to the major heat shock proteins, Hsp70 and Hsp90, and appears in early receptor complexes (1,2). Through mutual binding to both Hsp70 and Hsp90, Hop functions as an adaptor that can integrate Hsp70 and Hsp90 interactions (3,4). HOP is an abundant and highly conserved protein which is composed of three tetratricopeptide repeat (TPR) domains (TPR1, TPR2a and TPR2b) and two DP repeat domains (DP1 and DP2), whose function has not been fully resolved (5).

Sequence:

P31948 STIP1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEQVNELKEKGNKALSVGNIDDALQCYSEAIKLDPHNHVLYSNRSAAYAKKGDYQKAYEDGCKTVDLKPDWGKGYSRKAAALEFLNRFEEAKRTYEEGLKHEANNPQLKEGLQNMEARLAERKFMNPFNMPNLYQKLESDPRTRTLLSDPTYRELIEQLRNKPSDLGTKLQDPRIMTTLSVLLGVDLGSMDEEEEIATPPPPPPPKKETKPEPMEEDLPENKKQALKEKELGNDAYKKKDFDTALKHYDKAKELDPTNMTYITNQAAVYFEKGDYNKCRELCEKAIEVGRENREDYRQIAKAYARIGNSYFKEEKYKDAIHFYNKSLAEHRTPDVLKKCQQAEKILKEQERLAYINPDLALEEKNKGNECFQKGDYPQAMKHYTEAIKRNPKDAKLYSNRAACYTKLLEFQLALKDCEECIQLEPTFIKGYTRKAAALEAMKDYTKAMDVYQKALDLDSSCKEAADGYQRCMMAQYNRHDSPEDVKRRAMADPEVQQIMSDPAMRLILEQMQKDPQALSEHLKNPVIAQKIQKLMDVGLIAIR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Bovine

Sheep

Dog

Rabbit

Zebrafish

Xenopus

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P31948 As Substrate

P31948

Site	PTM Type	Enzyme	Source
M1	Acetylation		Uniprot
K8	Acetylation		Uniprot
K10	Acetylation		Uniprot
K13	Ubiquitination		Uniprot
S16	Phosphorylation		Uniprot
C26	S-Nitrosylation		Uniprot
K32	Ubiquitination		Uniprot
Y41	Phosphorylation		Uniprot
S42	Phosphorylation		Uniprot
R44	Methylation		Uniprot
Y48	Phosphorylation		Uniprot
Y54	Phosphorylation		Uniprot
Y58	Phosphorylation		Uniprot
C62	S-Nitrosylation		Uniprot
K68	Acetylation		Uniprot
K68	Ubiquitination		Uniprot
K73	Acetylation		Uniprot
K73	Sumoylation		Uniprot
K73	Ubiquitination		Uniprot
K78	Ubiquitination		Uniprot
R87	Methylation		Uniprot
R93	Methylation		Uniprot
Y95	Phosphorylation		Uniprot
K100	Acetylation		Uniprot
K100	Ubiquitination		Uniprot
K109	Ubiquitination		Uniprot
R118	Methylation		Uniprot
K123	Sumoylation		Uniprot
K123	Ubiquitination		Uniprot
K136	Ubiquitination		Uniprot
T145	Phosphorylation		Uniprot
S148	Phosphorylation		Uniprot
Y152	Phosphorylation		Uniprot
K162	Ubiquitination		Uniprot
K169	Ubiquitination		Uniprot
T198	Phosphorylation	P06493 (CDK1)	Uniprot
K207	Acetylation		Uniprot
K210	Acetylation		Uniprot
K210	Sumoylation		Uniprot
K210	Ubiquitination		Uniprot
K229	Acetylation		Uniprot
K237	Acetylation		Uniprot
K246	Acetylation		Uniprot
K246	Ubiquitination		Uniprot
K252	Ubiquitination		Uniprot
Y261	Phosphorylation		Uniprot
Y269	Phosphorylation		Uniprot
Y275	Phosphorylation		Uniprot
K284	Acetylation		Uniprot
K284	Ubiquitination		Uniprot
R293	Methylation		Uniprot
K301	Acetylation		Uniprot
K301	Ubiquitination		Uniprot
K312	Acetylation		Uniprot
K312	Methylation		Uniprot
K317	Ubiquitination		Uniprot
K325	Acetylation		Uniprot
K325	Methylation		Uniprot
K325	Ubiquitination		Uniprot
T332	Phosphorylation	P06493 (CDK1)	Uniprot
K337	Acetylation		Uniprot
K337	Ubiquitination		Uniprot
K344	Acetylation		Uniprot
K344	Ubiquitination		Uniprot
K347	Ubiquitination		Uniprot
Y354	Phosphorylation		Uniprot
K364	Acetylation		Uniprot
K364	Ubiquitination		Uniprot
K373	Acetylation		Uniprot
K373	Ubiquitination		Uniprot
K381	Acetylation		Uniprot
Y383	Phosphorylation		Uniprot
T384	Phosphorylation		Uniprot
K388	Acetylation		Uniprot
K388	Ubiquitination		Uniprot
K395	Acetylation		Uniprot
C417	S-Nitrosylation		Uniprot
C420	S-Nitrosylation		Uniprot
K429	Acetylation		Uniprot
K429	Ubiquitination		Uniprot
K434	Ubiquitination		Uniprot
K442	Ubiquitination		Uniprot
Y444	Phosphorylation		Uniprot
K446	Acetylation		Uniprot
K446	Ubiquitination		Uniprot
K453	Ubiquitination		Uniprot
C461	S-Nitrosylation		Uniprot
K462	Acetylation		Uniprot
K462	Ubiquitination		Uniprot
Y476	Phosphorylation		Uniprot
S481	Phosphorylation		Uniprot
K486	Ubiquitination		Uniprot
K513	Acetylation		Uniprot
K513	Ubiquitination		Uniprot
K523	Ubiquitination		Uniprot
K530	Acetylation		Uniprot
K530	Ubiquitination		Uniprot
K533	Ubiquitination		Uniprot
R543	Methylation		Uniprot

Research Backgrounds

P31948_STIP1_HUMAN

Function:

Acts as a co-chaperone for HSP90AA1. Mediates the association of the molecular chaperones HSPA8/HSC70 and HSP90 (By similarity).

Subcellular Location:

Cytoplasm. Nucleus.

Subunit Structure:

Probably forms a complex composed of chaperones HSP90 and HSP70, co-chaperones STIP1/HOP, CDC37, PPP5C, PTGES3/p23, TSC1 and client protein TSC2. Forms a complex with HSPA8/HSC70, HSPCA/HSP-86 and HSPCB/HSP-84 (By similarity). Interacts with PACRG. Interacts with METTL21B. Interacts with HSP90/HSP90AA1; the interaction dissociates the PPP5C:HSP90AA1 interaction. Interacts with FLCN, FNIP1 and FNIP2. Interacts with HSPA8/HSC70 (By similarity). Interacts with HSP90AB1; upon SMYD2-dependent HSP90AB1 methylation.

Family&Domains:

The TPR 1 repeat interacts with the C-terminal of HSC70. The TPR 4, 5 and 6 repeats (also called TPR2A domain) and TPR 7, 8 and 9 repeats (also called TPR2B domain) interact with HSP90.

Research Fields

· Human Diseases > Neurodegenerative diseases > Prion diseases.

References

1). Comparative proteomics identify HSP90A, STIP1 and TAGLN‑2 in serum extracellular vesicles as potential circulating biomarkers for human adenomyosis. Experimental and Therapeutic Medicine (PubMed: 35495589) [IF=2.7]

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

STIP1 Antibody - #DF6351