BHMT Antibody - #DF6348

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Product: BHMT Antibody
Catalog: DF6348
Description: Rabbit polyclonal antibody to BHMT
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Dog, Chicken
Mol.Wt.: 47kDa; 45kD(Calculated).
Uniprot: Q93088
RRID: AB_2838312

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(89%), Horse(89%), Dog(100%), Chicken(89%)
Clonality:
Polyclonal
Specificity:
BHMT Antibody detects endogenous levels of total BHMT.
RRID:
AB_2838312
Cite Format: Affinity Biosciences Cat# DF6348, RRID:AB_2838312.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Betaine homocysteine methyltransferase; Betaine homocysteine S methyltransferase 1; Betaine homocysteine S methyltransferase; Betaine--homocysteine S-methyltransferase 1; BHMT; BHMT1; BHMT1_HUMAN; EC 2.1.1.5; Epididymis secretory sperm binding protein Li 61p; HEL S 61p;

Immunogens

Immunogen:
Uniprot:

Q93088(BHMT1_HUMAN) >>Visit HPA database.

Gene(ID):
BHMT(635)
Expression:
Q93088 BHMT1_HUMAN:

Found exclusively in liver and kidney.

Description:
This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq, Jul 2008]
Sequence:
MPPVGGKKAKKGILERLNAGEIVIGDGGFVFALEKRGYVKAGPWTPEAAVEHPEAVRQLHREFLRAGSNVMQTFTFYASEDKLENRGNYVLEKISGQEVNEAACDIARQVADEGDALVAGGVSQTPSYLSCKSETEVKKVFLQQLEVFMKKNVDFLIAEYFEHVEEAVWAVETLIASGKPVAATMCIGPEGDLHGVPPGECAVRLVKAGASIIGVNCHFDPTISLKTVKLMKEGLEAARLKAHLMSQPLAYHTPDCNKQGFIDLPEFPFGLEPRVATRWDIQKYAREAYNLGVRYIGGCCGFEPYHIRAIAEELAPERGFLPPASEKHGSWGSGLDMHTKPWVRARARKEYWENLRIASGRPYNPSMSKPDGWGVTKGTAELMQQKEATTEQQLKELFEKQKFKSQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Horse
89
Bovine
89
Chicken
89
Sheep
78
Rabbit
78
Xenopus
67
Zebrafish
44
Pig
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q93088 As Substrate

Site PTM Type Enzyme
T45 Phosphorylation
Y289 Phosphorylation
Y305 Phosphorylation
S330 Phosphorylation
K340 Methylation
Y363 Phosphorylation
S366 Phosphorylation
K369 Acetylation

Research Backgrounds

Function:

Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Found exclusively in liver and kidney.

Subunit Structure:

Homotetramer.

Research Fields

· Metabolism > Amino acid metabolism > Glycine, serine and threonine metabolism.

· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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