SHMT2 Antibody - #DF6347

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Product: SHMT2 Antibody
Catalog: DF6347
Description: Rabbit polyclonal antibody to SHMT2
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 55kDa; 56kD(Calculated).
Uniprot: P34897
RRID: AB_2838311

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(92%), Dog(100%), Xenopus(83%)
Clonality:
Polyclonal
Specificity:
SHMT2 Antibody detects endogenous levels of total SHMT2.
RRID:
AB_2838311
Cite Format: Affinity Biosciences Cat# DF6347, RRID:AB_2838311.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

GLY A+; GLYA; glycine auxotroph A, human complement for hamster; Glycine hydroxymethyltransferase; GLYM_HUMAN; mitochondrial; Serine hydroxymethyltransferase 2 (mitochondrial); Serine hydroxymethyltransferase; Serine hydroxymethyltransferase mitochondrial; Serine methylase; SHMT 2; SHMT; SHMT2;

Immunogens

Immunogen:
Uniprot:

P34897(GLYM_HUMAN) >>Visit HPA database.

Gene(ID):
SHMT2(6472)
Description:
This gene encodes the mitochondrial form of a pyridoxal phosphate-dependent enzyme that catalyzes the reversible reaction of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. The encoded product is primarily responsible for glycine synthesis. The activity of the encoded protein has been suggested to be the primary source of intracellular glycine. The gene which encodes the cytosolic form of this enzyme is located on chromosome 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
Sequence:
MLYFSLFWAARPLQRCGQLVRMAIRAQHSNAAQTQTGEANRGWTGQESLSDSDPEMWELLQREKDRQCRGLELIASENFCSRAALEALGSCLNNKYSEGYPGKRYYGGAEVVDEIELLCQRRALEAFDLDPAQWGVNVQPYSGSPANLAVYTALLQPHDRIMGLDLPDGGHLTHGYMSDVKRISATSIFFESMPYKLNPKTGLIDYNQLALTARLFRPRLIIAGTSAYARLIDYARMREVCDEVKAHLLADMAHISGLVAAKVIPSPFKHADIVTTTTHKTLRGARSGLIFYRKGVKAVDPKTGREIPYTFEDRINFAVFPSLQGGPHNHAIAAVAVALKQACTPMFREYSLQVLKNARAMADALLERGYSLVSGGTDNHLVLVDLRPKGLDGARAERVLELVSITANKNTCPGDRSAITPGGLRLGAPALTSRQFREDDFRRVVDFIDEGVNIGLEVKSKTAKLQDFKSFLLKDSETSQRLANLRQRVEQFARAFPMPGFDEH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Zebrafish
100
Bovine
92
Sheep
92
Rabbit
92
Xenopus
83
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P34897 As Substrate

Site PTM Type Enzyme
Y3 Phosphorylation
S90 Phosphorylation
K95 Acetylation
K95 Ubiquitination
Y96 Phosphorylation
Y100 Phosphorylation
K103 Acetylation
K103 Ubiquitination
S178 Phosphorylation
K181 Acetylation
K181 Ubiquitination
S184 Phosphorylation
S192 Phosphorylation
K196 Acetylation
K200 Acetylation
K200 Ubiquitination
T212 Phosphorylation
Y228 Phosphorylation
R230 Methylation
R236 Methylation
K245 Acetylation
K262 Ubiquitination
S266 Phosphorylation
K269 Acetylation
K269 Methylation
K269 Ubiquitination
K280 Ubiquitination
K297 Acetylation
K297 Ubiquitination
K302 Acetylation
Y350 Phosphorylation
K356 Acetylation
K356 Ubiquitination
R368 Methylation
K389 Ubiquitination
S404 Phosphorylation
K409 Acetylation
K409 Ubiquitination
R416 Methylation
T420 Phosphorylation
R442 Methylation
K459 Ubiquitination
K464 Acetylation
K464 Ubiquitination
K469 Acetylation
K469 Methylation
K469 Ubiquitination
S470 Phosphorylation
K474 Acetylation
K474 Ubiquitination

Research Backgrounds

Function:

Catalyzes the cleavage of serine to glycine accompanied with the production of 5,10-methylenetetrahydrofolate, an essential intermediate for purine biosynthesis. Serine provides the major source of folate one-carbon in cells by catalyzing the transfer of one carbon from serine to tetrahydrofolate. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway via its role in glycine and tetrahydrofolate metabolism: thymidylate biosynthesis is required to prevent uracil accumulation in mtDNA. Also required for mitochondrial translation by producing 5,10-methylenetetrahydrofolate; 5,10-methylenetetrahydrofolate providing methyl donors to produce the taurinomethyluridine base at the wobble position of some mitochondrial tRNAs. Associates with mitochondrial DNA. In addition to its role in mitochondria, also plays a role in the deubiquitination of target proteins as component of the BRISC complex: required for IFNAR1 deubiquitination by the BRISC complex.

PTMs:

Succinylation at Lys-280 inhibits the hydroxymethyltransferase activity. Desuccinylation by SIRT5 restores the activity, leading to promote cell proliferation.

Subcellular Location:

Mitochondrion. Mitochondrion matrix>Mitochondrion nucleoid. Mitochondrion inner membrane. Cytoplasm. Nucleus.
Note: Mainly localizes in the mitochondrion. Also found in the cytoplasm and nucleus as part of the BRISC complex (PubMed:24075985).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homotetramer; in the presence of bound pyridoxal 5'-phosphate. Homodimer; in the absence of bound pyridoxal 5'-phosphate. Pyridoxal 5'-phosphate binding mediates an important conformation change that is required for tetramerization. Interacts with ABRAXAS2; the interaction is direct. Identified in a complex with ABRAXAS2 and the other subunits of the BRISC complex, at least composed of the ABRAXAS2, BRCC3/BRCC36, BABAM2 and BABAM1/NBA1. Identified in a complex with ABRAXAS2 and IFNAR1. Interacts with KIRREL3.

Family&Domains:

Belongs to the SHMT family.

Research Fields

· Human Diseases > Drug resistance: Antineoplastic > Antifolate resistance.

· Metabolism > Amino acid metabolism > Glycine, serine and threonine metabolism.

· Metabolism > Carbohydrate metabolism > Glyoxylate and dicarboxylate metabolism.

· Metabolism > Metabolism of cofactors and vitamins > One carbon pool by folate.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

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