ECI1 Antibody | Affinity Biosciences

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Western blot analysis of extracts from Mouse kidney, using ECI1 Antibody.

Product:	ECI1 Antibody
Catalog:	DF6343
Description:	Rabbit polyclonal antibody to ECI1
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Horse, Sheep, Rabbit, Chicken, Xenopus
Mol.Wt.:	33kDa; 33kD(Calculated).
Uniprot:	P42126
RRID:	AB_2838307

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Horse(100%), Sheep(89%), Rabbit(92%), Chicken(92%), Xenopus(92%)

Clonality:

Polyclonal

Specificity:

ECI1 Antibody detects endogenous levels of total ECI1.

RRID:

AB_2838307
Cite Format: Affinity Biosciences Cat# DF6343, RRID:AB_2838307.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

2-trans-enoyl-CoA isomerase; 3; 3,2 trans enoyl Coenzyme A isomerase; 3,2 trans-enoyl-CoA isomerase; 3,2-trans-enoyl-CoA isomerase, mitochondrial; Acetylene allene isomerase; D2-enoyl-CoA isomerase; D3; D3,D2 enoyl CoA isomerase; DCI; DCI protein; delta(2)-enoyl-CoA isomerase; Delta(3); Delta(3),Delta(2) enoyl CoA isomerase; Delta3, delta2 enoyl CoA isomerase; Dodecenoyl CoA delta Isomerase; Dodecenoyl CoA isomerase; Dodecenoyl Coenzyme A delta isomerase; Dodecenoyl Coenzyme A delta isomerase (3,2 trans enoyl Coenzyme A isomerase); Dodecenoyl Coenzyme A delta isomerase 3,2 trans enoyl Coenzyme A isomerase, isoform CRA a; Dodecenoyl-CoA isomerase; ECI1; ECI1_HUMAN; Enoyl-CoA delta isomerase 1; enoyl-CoA delta isomerase 1, mitochondrial; mitochondrial;

Immunogens

Immunogen:

Uniprot:

P42126(ECI1_HUMAN) >>Visit HPA database.

Gene(ID):

ECI1(1632)

Description:

This gene encodes a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2010]

Sequence:

P42126 ECI1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MALVASVRVPARVLLRAGARLPGAALGRTERAAGGGDGARRFGSQRVLVEPDAGAGVAVMKFKNPPVNSLSLEFLTELVISLEKLENDKSFRGVILTSDRPGVFSAGLDLTEMCGRSPAHYAGYWKAVQELWLRLYQSNLVLVSAINGACPAGGCLVALTCDYRILADNPRYCIGLNETQLGIIAPFWLKDTLENTIGHRAAERALQLGLLFPPAEALQVGIVDQVVPEEQVQSTALSAIAQWMAIPDHARQLTKAMMRKATASRLVTQRDADVQNFVSFISKDSIQKSLQMYLERLKEEKG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Xenopus

Chicken

Rabbit

Sheep

Pig

Bovine

Dog

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P42126 As Substrate

P42126

Site	PTM Type	Source
K61	Ubiquitination	Uniprot
K89	Acetylation	Uniprot
R171	Methylation	Uniprot
S279	Phosphorylation	Uniprot
S282	Phosphorylation	Uniprot
K283	Acetylation	Uniprot
K283	Ubiquitination	Uniprot
S285	Phosphorylation	Uniprot
K288	Acetylation	Uniprot
K288	Ubiquitination	Uniprot

Research Backgrounds

P42126_ECI1_HUMAN

Function:

Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species.

Subcellular Location:

Mitochondrion matrix.

Subunit Structure:

Homotrimer.

Family&Domains:

Belongs to the enoyl-CoA hydratase/isomerase family.

Research Fields

· Metabolism > Lipid metabolism > Fatty acid degradation.

Restrictive clause

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For Research Use Only.
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ECI1 Antibody - #DF6343