UGDH Antibody - #DF6342

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Product: UGDH Antibody
Catalog: DF6342
Description: Rabbit polyclonal antibody to UGDH
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 55kDa; 55kD(Calculated).
Uniprot: O60701
RRID: AB_2838306

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(89%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
UGDH Antibody detects endogenous levels of total UGDH.
RRID:
AB_2838306
Cite Format: Affinity Biosciences Cat# DF6342, RRID:AB_2838306.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

GDH; UDP Glc dehydrogenase; UDP GlcDH; UDP glucose 6 dehydrogenase; UDP-Glc dehydrogenase; UDP-GlcDH; UDP-glucose 6-dehydrogenase; UDP-glucose dehydrogenase; UDPGDH; UGD; Ugdh; UGDH_HUMAN; Uridine diphospho glucose dehydrogenase;

Immunogens

Immunogen:
Uniprot:

O60701(UGDH_HUMAN) >>Visit HPA database.

Gene(ID):
UGDH(7358)
Expression:
O60701 UGDH_HUMAN:

Detected in heart, placenta, liver, pancreas, spleen, thymus, prostate, ovary, small intestine and colon (PubMed:9737970). Widely expressed (PubMed:9737970).

Description:
UDP-GlcDH (also called UDP-glucose 6-dehydrogenase, UGDH or UDPGDH) is a member of the UDP-glucose/GDP-mannose dehydrogenase family. UDP-GlcDH converts UDP-glucose to UDP-glucuronic acid, which is a crucial component in the biosynthesis of the glycosaminoglycans, hyaluronan, heparan sulfate and chondroitin sulfate. Found as common components of the extracellular matrix, these glycosaminoglycans are significant in signal transduction, cell migration, cancer growth and cancer metastasis. UDP-glucuronic acid (UDP-GlcA) is needed in the liver for the excretion of toxic compounds. UDP-GlcDH is a ubiquitously expressed protein most abundant in the liver. The protein structure of UDP-GlcDH was first analyzed in cow liver and found to be a homohexamer. This structure is well conserved between species and phyla with an overall 97% sequence identity shared between different species of mammals. Research indicates that UDP-GlcDH expression is upregulated by TGF∫ and downregulated by hypoxia.
Sequence:
MFEIKKICCIGAGYVGGPTCSVIAHMCPEIRVTVVDVNESRINAWNSPTLPIYEPGLKEVVESCRGKNLFFSTNIDDAIKEADLVFISVNTPTKTYGMGKGRAADLKYIEACARRIVQNSNGYKIVTEKSTVPVRAAESIRRIFDANTKPNLNLQVLSNPEFLAEGTAIKDLKNPDRVLIGGDETPEGQRAVQALCAVYEHWVPREKILTTNTWSSELSKLAANAFLAQRISSINSISALCEATGADVEEVATAIGMDQRIGNKFLKASVGFGGSCFQKDVLNLVYLCEALNLPEVARYWQQVIDMNDYQRRRFASRIIDSLFNTVTDKKIAILGFAFKKDTGDTRESSSIYISKYLMDEGAHLHIYDPKVPREQIVVDLSHPGVSEDDQVSRLVTISKDPYEACDGAHAVVICTEWDMFKELDYERIHKKMLKPAFIFDGRRVLDGLHNELQTIGFQIETIGKKVSSKRIPYAPSGEIPKFSLQDPPNKKPKV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
89
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O60701 As Substrate

Site PTM Type Enzyme
S40 Phosphorylation
S47 Phosphorylation
Y53 Phosphorylation
K67 Ubiquitination
K80 Ubiquitination
S88 Phosphorylation
T91 Phosphorylation
K100 Ubiquitination
K107 Acetylation
K107 Ubiquitination
Y108 Phosphorylation
K124 Ubiquitination
K129 Ubiquitination
K149 Ubiquitination
K170 Ubiquitination
K173 Acetylation
K173 Ubiquitination
T185 Phosphorylation
Y199 Phosphorylation
K207 Ubiquitination
K264 Ubiquitination
K267 Ubiquitination
S275 Phosphorylation
Y286 Phosphorylation
Y309 Phosphorylation
S316 Phosphorylation
S321 Phosphorylation
T327 Phosphorylation
K339 Acetylation
Y352 Phosphorylation
S354 Phosphorylation
K370 Ubiquitination
S386 Phosphorylation
K430 Ubiquitination
K434 Acetylation
T461 Phosphorylation P06493 (CDK1)
Y473 Phosphorylation
S476 Phosphorylation
S483 Phosphorylation
K490 Acetylation

Research Backgrounds

Function:

Catalyzes the formation of UDP-alpha-D-glucuronate, a constituent of complex glycosaminoglycans. Required for the biosynthesis of chondroitin sulfate and heparan sulfate. Required for embryonic development via its role in the biosynthesis of glycosaminoglycans (By similarity).

Tissue Specificity:

Detected in heart, placenta, liver, pancreas, spleen, thymus, prostate, ovary, small intestine and colon. Widely expressed.

Subunit Structure:

Homohexamer.

Family&Domains:

The protein goes through several conformation states during the reaction cycle, giving rise to hysteresis. In the initial state, the ligand-free protein is in an inactive conformation (E*). Substrate binding triggers a change to the active conformation (E). UDP-xylose binding triggers the transition to a distinct, inhibited conformation. The presence of an intrinsically disordered C-terminus promotes a more dynamic protein structure and favors a conformation with high affinity for UPD-xylose.

The allosteric switch region moves by about 5 Angstroms when UDP-xylose is bound, and occupies part of the UDP-glucose binding site. At the same time it promotes domain movements that disrupt the active hexameric ring structure and lead to the formation of a horseshoe-shaped, inactive hexamer.

Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.

Research Fields

· Metabolism > Carbohydrate metabolism > Pentose and glucuronate interconversions.

· Metabolism > Carbohydrate metabolism > Ascorbate and aldarate metabolism.

· Metabolism > Carbohydrate metabolism > Amino sugar and nucleotide sugar metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

 

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