TPM3 Antibody - #DF6338

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Product: TPM3 Antibody
Catalog: DF6338
Description: Rabbit polyclonal antibody to TPM3
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 33kDa; 33kD(Calculated).
Uniprot: P06753
RRID: AB_2838302

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
TPM3 Antibody detects endogenous levels of total TPM3.
RRID:
AB_2838302
Cite Format: Affinity Biosciences Cat# DF6338, RRID:AB_2838302.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Alpha tropomyosin 3; Alpha tropomyosin slow skeletal; CFTD; Cytoskeletal tropomyosin TM30; FLJ41118; gamma TM; Gamma tropomyosin; Gamma-tropomyosin; Heat stable cytoskeletal protein 30 kDa; hscp30; hTM30nm; hTM5; hTMnm; MGC102590; MGC14582; MGC3261; MGC72094; NEM1; OK/SW-cl.5; OTTHUMP00000034019; OTTHUMP00000034171; OTTHUMP00000034172; TM 5; TM-5; TM3; TM30; TM30nm; TM5; Tm5NM; Tpm 5; TPM3; TPM3/NTRK1 FUSION GENE, INCLUDED; TPM3_HUMAN; Tpm5; TPMsk3; TRK; TRK ONCOGENE, INCLUDED; Trop 5; Tropomyosin 3; Tropomyosin 3 gamma; Tropomyosin 5; Tropomyosin alpha 3 chain; Tropomyosin alpha-3 chain; Tropomyosin gamma; Tropomyosin-3; Tropomyosin-5;

Immunogens

Immunogen:
Uniprot:

P06753(TPM3_HUMAN) >>Visit HPA database.

Gene(ID):
TPM3(7170)
Description:
This gene encodes a member of the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosins are dimers of coiled-coil proteins that polymerize end-to-end along the major groove in most actin filaments. They provide stability to the filaments and regulate access of other actin-binding proteins. In muscle cells, they regulate muscle contraction by controlling the binding of myosin heads to the actin filament. Mutations in this gene result in autosomal dominant nemaline myopathy, and oncogenes formed by chromosomal translocations involving this locus are associated with cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Sequence:
MMEAIKKKMQMLKLDKENALDRAEQAEAEQKQAEERSKQLEDELAAMQKKLKGTEDELDKYSEALKDAQEKLELAEKKAADAEAEVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLERTEERAELAESKCSELEEELKNVTNNLKSLEAQAEKYSQKEDKYEEEIKILTDKLKEAETRAEFAERSVAKLEKTIDDLEDELYAQKLKYKAISEELDHALNDMTSI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Zebrafish
67
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P06753 As Substrate

Site PTM Type Enzyme
K13 Acetylation
K13 Ubiquitination
K16 Acetylation
K16 Ubiquitination
K49 Acetylation
K50 Acetylation
K60 Ubiquitination
Y61 Phosphorylation
S62 Phosphorylation
S88 Phosphorylation
T109 Phosphorylation
K113 Acetylation
K113 Ubiquitination
K119 Acetylation
K119 Ubiquitination
S124 Phosphorylation
K129 Sumoylation
K129 Ubiquitination
K137 Acetylation
K137 Ubiquitination
K141 Acetylation
K150 Acetylation
K153 Acetylation
K153 Ubiquitination
R161 Methylation
K162 Acetylation
Y163 Phosphorylation
K169 Ubiquitination
R179 Methylation
S189 Phosphorylation
S192 Phosphorylation
T202 Phosphorylation
S207 Phosphorylation
K214 Ubiquitination
S216 Phosphorylation
K218 Ubiquitination
K221 Acetylation
K221 Methylation
K221 Ubiquitination
Y222 Phosphorylation
K227 Ubiquitination
K232 Ubiquitination
K234 Ubiquitination
T238 Phosphorylation
S246 Phosphorylation
K249 Acetylation
K249 Ubiquitination
T253 Phosphorylation
Y262 Phosphorylation
K265 Ubiquitination
K269 Ubiquitination
S272 Phosphorylation
T283 Phosphorylation
S284 Phosphorylation

Research Backgrounds

Function:

Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.

Subcellular Location:

Cytoplasm>Cytoskeleton.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer. Heterodimer of an alpha (TPM1, TPM3 or TPM4) and a beta (TPM2) chain (By similarity). Interacts with TMOD1.

Family&Domains:

The molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity.

Belongs to the tropomyosin family.

Research Fields

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Thyroid cancer.   (View pathway)

· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).

· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).

· Organismal Systems > Circulatory system > Cardiac muscle contraction.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

References

1). Physical interaction of tropomyosin 3 and STIM1 regulates vascular smooth muscle contractility and contributes to hypertension. BIOMEDICINE & PHARMACOTHERAPY, 2021 (PubMed: 33341060) [IF=7.5]

Application: WB    Species: Rat    Sample: vascular smooth muscle cell (VSMC)

Fig. 1. STIM1 and TPM3 Interact and colocalization. A) Five genes are shown having a correlation coefficient with STIM1 greater than 0.7. The X-axis represents the results of each of 80 samples across 11 organs and four age groups; the Y-axis represents the expression levels of the five genes in each sample. Expression levels of TPM3 are highly correlated with those of STIM1, with both proteins highly expressed in numerous tissues and organs. B and C) Representative images of a coimmunoprecipitation (co-IP) experiment. Panel B shows that protein from the mesenteric resistance artery (MRA) is precipitated by a STIM1 antibody and detected by a TPM3 antibody. Panel C shows that protein from the MRA is precipitated by a TPM3 antibody and detected by a STIM1 antibody. Panels B and C also show immunoblots (IB) of cell lysates. D and E) Identification of primary cultured vascular smooth muscle cells (VSMCs). Positive green fluorescence is observed in VSMCs treated with an antibody to smooth muscle α-actin (α-SMA) (D). No immunofluorescence is observed in the negative control (Phosphate Buffered Saline, replaced primary antibody in E). F) Colocalization of STIM1 and TPM3 in VSMCs. Red fluorescence represents TPM3; green fluorescence represents STIM1; blue fluorescence refers nucleus. Merged image indicates STIM1 and TPM3 co-localization. FPKM represents fragments per kilobase of exon model per million reads mapped (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article).

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