SERPINB5 Antibody - #DF6313

(5)  
Product: SERPINB5 Antibody
Catalog: DF6313
Description: Rabbit polyclonal antibody to SERPINB5
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 42kDa; 42kD(Calculated).
Uniprot: P36952
RRID: AB_2838279

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(91%), Dog(100%)
Clonality:
Polyclonal
Specificity:
SERPINB5 Antibody detects endogenous levels of total SERPINB5.
RRID:
AB_2838279
Cite Format: Affinity Biosciences Cat# DF6313, RRID:AB_2838279.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Maspin; Ovalbumin; Peptidase inhibitor 5; PI-5; PI5; protease inhibitor 5 (maspin); Protease inhibitor 5; Serine (or cysteine) peptidase inhibitor, clade B, member 5; serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5; Serpin B5; serpin family B member 5; Serpin peptidase inhibitor; Serpin peptidase inhibitor, clade B (ovalbumin), member 5; Serpinb5; SPB5_HUMAN; Spi5; Spi7;

Immunogens

Immunogen:
Uniprot:

P36952(SPB5_HUMAN) >>Visit HPA database.

Gene(ID):
SERPINB5(5268)
Expression:
P36952 SPB5_HUMAN:

Normal mammary epithelial cells.

Description:
Maspin (SERPINB5) was discovered as a mammary tumor suppressor that is expressed in normal mammary epithelium but lost in most breast cancer cell lines (1). While maspin is related to the serpin family of serine protease inhibitors, it may not function as a protease inhibitor (2). It plays an essential role in embryonic development through critical roles in cell adhesion (3). While the precise mechanism of maspin signaling is unclear (4), the tumor suppressing activity of maspin has been attributed to its ability to inhibit cell invasion/metastasis (5,6) and angiogenesis (7), while promoting apoptosis (8). Nuclear translocation of active IKKα has been shown to repress maspin transcription and promote prostate cancer metastasis (9).
Sequence:
MDALQLANSAFAVDLFKQLCEKEPLGNVLFSPICLSTSLSLAQVGAKGDTANEIGQVLHFENVKDVPFGFQTVTSDVNKLSSFYSLKLIKRLYVDKSLNLSTEFISSTKRPYAKELETVDFKDKLEETKGQINNSIKDLTDGHFENILADNSVNDQTKILVVNAAYFVGKWMKKFSESETKECPFRVNKTDTKPVQMMNMEATFCMGNIDSINCKIIELPFQNKHLSMFILLPKDVEDESTGLEKIEKQLNSESLSQWTNPSTMANAKVKLSIPKFKVEKMIDPKACLENLGLKHIFSEDTSDFSGMSETKGVALSNVIHKVCLEITEDGGDSIEVPGARILQHKDELNADHPFIYIIRHNKTRNIIFFGKFCSP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
91
Chicken
64
Xenopus
45
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P36952 As Substrate

Site PTM Type Enzyme
S9 Phosphorylation
T50 Phosphorylation
K64 Ubiquitination
K79 Ubiquitination
S85 Phosphorylation
Y93 Phosphorylation
S97 Phosphorylation
K109 Ubiquitination
K114 Acetylation
K114 Ubiquitination
T118 Phosphorylation
K129 Ubiquitination
S135 Phosphorylation
K137 Ubiquitination
T157 Phosphorylation
S240 Phosphorylation
T241 Phosphorylation
K245 Ubiquitination
K248 Ubiquitination
K275 Ubiquitination
K277 Acetylation
K280 Acetylation
K285 Ubiquitination
S298 Phosphorylation
S302 Phosphorylation
T310 Phosphorylation
K311 Ubiquitination
S316 Phosphorylation
K345 Ubiquitination
Y356 Phosphorylation

Research Backgrounds

Function:

Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.

Subcellular Location:

Secreted>Extracellular space.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Normal mammary epithelial cells.

Subunit Structure:

Interacts with IRF6.

Family&Domains:

Belongs to the serpin family. Ov-serpin subfamily.

Research Fields

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

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