KCNE1 Antibody - #DF6310

(12)  
Product: KCNE1 Antibody
Catalog: DF6310
Description: Rabbit polyclonal antibody to KCNE1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep
Mol.Wt.: 15kDa; 15kD(Calculated).
Uniprot: P15382
RRID: AB_2838276

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Bovine(82%), Horse(91%), Sheep(82%)
Clonality:
Polyclonal
Specificity:
KCNE1 Antibody detects endogenous levels of total KCNE1.
RRID:
AB_2838276
Cite Format: Affinity Biosciences Cat# DF6310, RRID:AB_2838276.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Delayed rectifier potassium channel subunit IsK; Human cardiac delayed rectifier potassium channel protein; IKs producing slow voltage gated potassium channel subunit beta Mink; IKs producing slow voltage-gated potassium channel subunit beta Mink; ISK; JLNS 2; JLNS; JLNS2; KCNE 1; KCNE1; KCNE1_HUMAN; LQT 5; LQT5; MGC33114; Minimal potassium channel; MinK; Potassium voltage gated channel Isk related family member 1; Potassium voltage gated channel Isk related subfamily member 1; Potassium voltage gated channel subfamily E member 1; Potassium voltage-gated channel subfamily E member 1;

Immunogens

Immunogen:
Uniprot:

P15382(KCNE1_HUMAN) >>Visit HPA database.

Gene(ID):
KCNE1(3753)
Expression:
P15382 KCNE1_HUMAN:

Expressed in lung, kidney, testis, ovaries, small intestine, peripheral blood leukocytes. Expressed in the heart (PubMed:19219384). Not detected in pancreas, spleen, prostate and colon. Restrictively localized in the apical membrane portion of epithelial cells.

Description:
Voltage-gated potassium channels play a variety of important roles in human health and disease (1,2). KCNE1, also known as MinK, belongs to a family of small transmembrane proteins (KCNE1, 2, 3, 4, and KCNE1L) that modulate the activity of several voltage-gated K+ channels (3-5). KCNE1 functions as the modulatory β-subunit of the pore-forming α-subunit KCNQ1, and alters several biophysical properties of KCNQ1 channels (6,7). Research studies have shown that several inherited mutations in KCNE1 result in long QT syndrome (8-10) and deafness (11).
Sequence:
MILSNTTAVTPFLTKLWQETVQQGGNMSGLARRSPRSSDGKLEALYVLMVLGFFGFFTLGIMLSYIRSKKLEHSNDPFNVYIESDAWQEKDKAYVQARVLESYRSCYVVENHLAIEQPNTHLPETKPSP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
92
Horse
91
Bovine
82
Sheep
82
Rabbit
73
Xenopus
60
Dog
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P15382 As Substrate

Site PTM Type Enzyme
N5 N-Glycosylation
T7 O-Glycosylation
T7 Phosphorylation
N26 N-Glycosylation
S28 Phosphorylation
S102 Phosphorylation P17252 (PRKCA)

Research Backgrounds

Function:

Ancillary protein that assembles as a beta subunit with a voltage-gated potassium channel complex of pore-forming alpha subunits. Modulates the gating kinetics and enhances stability of the channel complex. Assembled with KCNB1 modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1. Assembled with KCNQ1/KVLQT1 is proposed to form the slowly activating delayed rectifier cardiac potassium (IKs) channel. The outward current reaches its steady state only after 50 seconds. Assembled with KCNH2/HERG may modulate the rapidly activating component of the delayed rectifying potassium current in heart (IKr).

PTMs:

Phosphorylation inhibits the potassium current.

N-glycosylation at Asn-26 occurs post-translationally, and requires prior cotranslational glycosylation at Asn-5.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein. Apical cell membrane. Membrane raft.
Note: Colocalizes with KCNB1 at the plasma membrane (By similarity). Targets to the membrane raft when associated with KCNQ1 (PubMed:20533308).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in lung, kidney, testis, ovaries, small intestine, peripheral blood leukocytes. Expressed in the heart. Not detected in pancreas, spleen, prostate and colon. Restrictively localized in the apical membrane portion of epithelial cells.

Subunit Structure:

Interacts with KCNB1. Interacts with KCNC2 (By similarity). Associates with KCNH2/HERG. Interacts with KNCQ1; targets the complex KNCQ1-KCNE1 to the membrane raft. The complex KNCQ1-KNCE1 interacts with the scolopendra toxin SSD609.

Family&Domains:

Belongs to the potassium channel KCNE family.

Research Fields

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.