FAAH Antibody | Affinity Biosciences

WB
IHC
IF/ICC
Cites

1/6

Western blot analysis of extracts from VERO, using FAAH Antibody.

DF6308 at 1/100 staining Mouse brain tissue by IHC-P.

DF6308 at 1/100 staining Mouse testis tissue by IHC-P.

DF6308 at 1/100 staining Rat brain tissue by IHC-P.

DF6308 staining A549 cells by IF/ICC.

Fig.

Product:	FAAH Antibody
Catalog:	DF6308
Description:	Rabbit polyclonal antibody to FAAH
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat, Monkey
Prediction:	Pig, Horse, Rabbit, Dog
Mol.Wt.:	63kDa; 63kD(Calculated).
Uniprot:	O00519
RRID:	AB_2838274

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat,Monkey

Prediction:

Pig(88%), Horse(100%), Rabbit(100%), Dog(89%)

Clonality:

Polyclonal

Specificity:

FAAH Antibody detects endogenous levels of total FAAH.

RRID:

AB_2838274
Cite Format: Affinity Biosciences Cat# DF6308, RRID:AB_2838274.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Anandamide amidohydrolase 1; Anandamide amidohydrolase; FA2H; FAAH; FAAH-1; FAAH1_HUMAN; Fatty acid 2-hydroxylase; Fatty acid alpha-hydroxylase; Fatty acid amide hydrolase; Fatty-acid amide hydrolase 1; MGC102823; MGC138146; Oleamide hydrolase 1; Oleamide hydrolase;

Immunogens

Immunogen:

Uniprot:

O00519(FAAH1_HUMAN) >>Visit HPA database.

Gene(ID):

FAAH(2166)

Expression:

O00519 FAAH1_HUMAN:

Highly expressed in the brain, small intestine, pancreas, skeletal muscle and testis. Also expressed in the kidney, liver, lung, placenta and prostate.

Description:

Endogenous cannabinoids have been implicated in addictive behaviors and drug abuse (1). Fatty-acid amide hydrolase 1 (FAAH1) is a plasma membrane-bound hydrolase that converts oleamide to oleic acid (2). This hydrolase also converts the cannabinoid anandamide, the endogenous ligand for the CB1 cannabinoid receptor, to arachidonic acid, suggesting a role in fatty-acid amide inactivation (2). Mice lacking FAAH1 have significantly higher levels of anandamide in the brain and show decreased sensitivity to pain, further indicating a role for FAAH1 in the regulation of endocannabinoid signaling in vivo (3). FAAH1 null mice also demonstrate an increased preference for alcohol and an increased voluntary uptake of alcohol as compared to wild-type mice, indicating a role of FAAH1 in modulating addictive behaviors (1).

Sequence:

O00519 FAAH1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVQYELWAALPGASGVALACCFVAAAVALRWSGRRTARGAVVRARQRQRAGLENMDRAAQRFRLQNPDLDSEALLALPLPQLVQKLHSRELAPEAVLFTYVGKAWEVNKGTNCVTSYLADCETQLSQAPRQGLLYGVPVSLKECFTYKGQDSTLGLSLNEGVPAECDSVVVHVLKLQGAVPFVHTNVPQSMFSYDCSNPLFGQTVNPWKSSKSPGGSSGGEGALIGSGGSPLGLGTDIGGSIRFPSSFCGICGLKPTGNRLSKSGLKGCVYGQEAVRLSVGPMARDVESLALCLRALLCEDMFRLDPTVPPLPFREEVYTSSQPLRVGYYETDNYTMPSPAMRRAVLETKQSLEAAGHTLVPFLPSNIPHALETLSTGGLFSDGGHTFLQNFKGDFVDPCLGDLVSILKLPQWLKGLLAFLVKPLLPRLSAFLSNMKSRSAGKLWELQHEIEVYRKTVIAQWRALDLDVVLTPMLAPALDLNAPGRATGAVSYTMLYNCLDFPAGVVPVTTVTAEDEAQMEHYRGYFGDIWDKMLQKGMKKSVGLPVAVQCVALPWQEELCLRFMREVERLMTPEKQSS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Horse

100

Dog

Pig

Bovine

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O00519 As Substrate

O00519

Site	PTM Type	Source
S88	Phosphorylation	Uniprot
T99	Phosphorylation	Uniprot
S241	Phosphorylation	Uniprot
S289	Phosphorylation	Uniprot
Y329	Phosphorylation	Uniprot
Y335	Phosphorylation	Uniprot
K423	Ubiquitination	Uniprot
S430	Phosphorylation	Uniprot
S434	Phosphorylation	Uniprot
S440	Phosphorylation	Uniprot
Y454	Phosphorylation	Uniprot

Research Backgrounds

O00519_FAAH1_HUMAN

Function:

Degrades bioactive fatty acid amides like oleamide, the endogenous cannabinoid, anandamide and myristic amide to their corresponding acids, thereby serving to terminate the signaling functions of these molecules. Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates.

Subcellular Location:

Endomembrane system>Single-pass membrane protein. Cytoplasm>Cytoskeleton.
Note: Seems to be attached to intracellular membranes and a portion of the cytoskeletal network.

Tissue Specificity:

Highly expressed in the brain, small intestine, pancreas, skeletal muscle and testis. Also expressed in the kidney, liver, lung, placenta and prostate.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the amidase family.

Research Fields

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling. (View pathway)

References

1). MicroRNA miR-1275 coordinately regulates AEA/LPA signals via targeting FAAH in lipid metabolism reprogramming of gastric cancer. Cell Death & Disease, 2023 (PubMed: 36702852) [IF=9.0]

Application: WB Species: Mouse Sample: GC tissues

Fig. 1: FAAH is highly expressed in GC tissues.A Clustered heatmap. Each row represents a tissue sample, and each column represents a coding gene. B Volcano plots. The red points on the right indicate the differentially upregulated genes with statistical significance while the red points on the left indicate the downregulated genes. Differential expression of FAAH in tissues from C TCGA database and D Oncomine database. E Differential expression analysis of FAAH in 4 GC subtypes (data from TCGA). F mRNA levels of FAAH in 96 pairs of GC tissues. G Protein levels of FAAH in 20 pairs of GC tissues. *p

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FAAH Antibody - #DF6308