RAB11A Antibody | Affinity Biosciences

Cites

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Figure 5 Exocyst trafficks MVBs by interacting with Rab11a (A) Exocyst interacts with Rab11a, as assessed using the STRING database.

Product:	RAB11A Antibody
Catalog:	DF6301
Description:	Rabbit polyclonal antibody to RAB11A
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	24kDa; 24kD(Calculated).
Uniprot:	P62491
RRID:	AB_2838267

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(86%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%), Xenopus(88%)

Clonality:

Polyclonal

Specificity:

RAB11A Antibody detects endogenous levels of total RAB11A.

RRID:

AB_2838267
Cite Format: Affinity Biosciences Cat# DF6301, RRID:AB_2838267.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

MGC1490; Rab 11; Rab 11A; RAB 11A, member oncogene family; Rab-11; RAB11 A; RAB11; Rab11a; RAB11A member RAS oncogene family; Ras related protein Rab 11A; Ras related protein Rab11A; Ras-related protein Rab-11A; RB11A_HUMAN; YL 8; YL8;

Immunogens

Immunogen:

Uniprot:

P62491(RB11A_HUMAN) >>Visit HPA database.

Gene(ID):

RAB11A(8766)

Description:

Rab11a, Rab11b and Rab25 are members of the Rab11 family of small Ras-like GTPases. Rab11 (isoforms Rab11a and Rab11b) functions as a key regulator in the recycling of perinuclear, plasma membrane and Golgi compartment endosomes (1,2). Despite some overlap, distinct differences exist between Rab11a and Rab11b in both their cellular distribution and functional roles. Rab11a is ubiquitously expressed while Rab11b is found mainly in the heart and brain (3,4). Like other Rab proteins, Rab11 exerts its function via interactions with Rab11 family interacting proteins (FIPs). While there are three distinct classes of FIPs, all appear to share a conserved carboxy-terminal Rab-binding domain that allows Rab-FIP protein interaction. When bound together, these proteins are thought to regulate membrane-associated protein sorting (5,6).

Sequence:

P62491 RB11A_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGTRDDEYDYLFKVVLIGDSGVGKSNLLSRFTRNEFNLESKSTIGVEFATRSIQVDGKTIKAQIWDTAGQERYRAITSAYYRGAVGALLVYDIAKHLTYENVERWLKELRDHADSNIVIMLVGNKSDLRHLRAVPTDEARAFAEKNGLSFIETSALDSTNVEAAFQTILTEIYRIVSQKQMSDRRENDMSPSNNVVPIHVPPTTENKPKVQCCQNI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Xenopus

Chicken

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P62491 As Substrate

P62491

Site	PTM Type	Enzyme	Source
T3	Phosphorylation		Uniprot
Y8	Phosphorylation		Uniprot
S20	Phosphorylation		Uniprot
K24	Methylation		Uniprot
K24	Ubiquitination		Uniprot
S40	Phosphorylation		Uniprot
S42	Phosphorylation		Uniprot
T43	Phosphorylation		Uniprot
T50	Phosphorylation		Uniprot
S52	Phosphorylation		Uniprot
K58	Ubiquitination		Uniprot
K61	Ubiquitination		Uniprot
Y73	Phosphorylation		Uniprot
R74	Methylation		Uniprot
S78	Phosphorylation		Uniprot
Y91	Phosphorylation		Uniprot
K95	Ubiquitination		Uniprot
T98	Phosphorylation		Uniprot
Y99	Phosphorylation		Uniprot
R104	Methylation		Uniprot
K107	Ubiquitination		Uniprot
S115	Phosphorylation		Uniprot
R132	Methylation		Uniprot
T136	Phosphorylation		Uniprot
K145	Ubiquitination		Uniprot
S177	Phosphorylation	P05771-2 (PRKCB) , Q02156 (PRKCE)	Uniprot
K179	Ubiquitination		Uniprot
S190	Phosphorylation		Uniprot
K207	Ubiquitination		Uniprot

Research Backgrounds

P62491_RB11A_HUMAN

Function:

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. The small Rab GTPase RAB11A regulates endocytic recycling. Acts as a major regulator of membrane delivery during cytokinesis. Together with MYO5B and RAB8A participates in epithelial cell polarization. Together with RAB3IP, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B participates in CFTR trafficking to the plasma membrane and TF (Transferrin) recycling in nonpolarized cells. Required in a complex with MYO5B and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Participates in the sorting and basolateral transport of CDH1 from the Golgi apparatus to the plasma membrane. Regulates the recycling of FCGRT (receptor of Fc region of monomeric Ig G) to basolateral membranes. May also play a role in melanosome transport and release from melanocytes.

Subcellular Location:

Cell membrane>Lipid-anchor. Recycling endosome membrane>Lipid-anchor. Cleavage furrow. Cytoplasmic vesicle>Phagosome. Cytoplasmic vesicle membrane.
Note: Translocates with RAB11FIP2 from the vesicles of the endocytic recycling compartment (ERC) to the plasma membrane (PubMed:11994279). Localizes to the cleavage furrow (PubMed:15601896). Colocalizes with PARD3, PRKCI, EXOC5, OCLN, PODXL and RAB8A in apical membrane initiation sites (AMIS) during the generation of apical surface and lumenogenesis (PubMed:20890297). Recruited to phagosomes containing S.aureus or M.tuberculosis (PubMed:21255211).

Subunit Structure:

Interacts with RIP11 and STXBP6. Interacts with SGSM1, SGSM2 and SGSM3 (By similarity). Interacts with EXOC6 in a GTP-dependent manner (By similarity). Interacts with RAB11FIP1, RAB11FIP2, RAB11FIP3 (via its C-terminus) and RAB11FIP4. Interacts with EVI5; EVI5 and RAB11FIP3 may be mutually exclusive and compete for binding RAB11A. Interacts with VIPAS39. Interacts with MYO5B. Found in a complex with MYO5B and CFTR. Interacts with NPC1L1. Interacts (GDP-bound form) with ZFYVE27. Interacts with BIRC6/bruce. May interact with TBC1D14. Interacts with UNC119; in a cell cycle-dependent manner. GDP-bound and nucleotide-free forms interact with SH3BP5. Interacts (GDP-bound form) with KIF5A in a ZFYVE27-dependent manner. Interacts (GDP-bound form) with RELCH (By similarity). Found in a complex composed of RELCH, OSBP1 and RAB11A (By similarity). Interacts with TBC1D12.

Family&Domains:

Belongs to the small GTPase superfamily. Rab family.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)

· Organismal Systems > Excretory system > Endocrine and other factor-regulated calcium reabsorption.

· Organismal Systems > Excretory system > Vasopressin-regulated water reabsorption.

· Organismal Systems > Digestive system > Pancreatic secretion.

References

1). Exocyst controls exosome biogenesis via Rab11a. Molecular Therapy-Nucleic Acids, 2022 (PubMed: 35036064) [IF=8.8]

Application: WB Species: Human Sample: HN4 and CNE2 cells

Figure 5 Exocyst trafficks MVBs by interacting with Rab11a (A) Exocyst interacts with Rab11a, as assessed using the STRING database. (B–E) Co-immunoprecipitation (co-IP) assay results showed that Sec10, Sec3, Exo70, and Rab11a interacted in HN4 and CNE2 cells. (F and G) Representative images of immunohistochemical staining (brown) (F) and summary data showing Rab11a expression in LC tissue versus adjacent healthy tissue (Ctrl) (G). ∗p < 0.05 versus siCtrl by t test. Scale bar, 50 μm (H and I) Exosome concentrations after the cells were transfected with scrambled or Rab11a siRNA in HN4 cells. (H) Representative nanoparticle tracking analysis traces. (I) The quantification of exosome concentrations in HN4 cells. ∗∗p < 0.01 versus siCtrl by t test. (J) Representative western blotting images show that CD9, CD63, and CD81 each interact with Rab11a, Exo70, Sec3, and Sec10 in HN4 and CNE2 cells. Lys, whole-cell lysates; Pre-immu, preimmune serum. (K–Z) The interactions between CD63 and Exo70, Sec3, Sec10, or Rab11a after siRNA-mediated of Exo70, Sec3, Sec10, or Rab11a knockdown. CD63 was used for normalization. ∗p < 0.05, ∗∗p < 0.01 versus siCtrl by t test.

Application: IHC Species: Human Sample: HN4 and CNE2 cells

2). Arf6-mediated macropinocytosis-enhanced suicide gene therapy of C16TAB-condensed Tat/pDNA nanoparticles in ovarian cancer. Nanoscale, 2021 (PubMed: 34473182) [IF=6.7]

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RAB11A Antibody - #DF6301