RAB11A Antibody - #DF6301

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Product: RAB11A Antibody
Catalog: DF6301
Description: Rabbit polyclonal antibody to RAB11A
Application: WB IHC
Cited expt.: WB, IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 24kDa; 24kD(Calculated).
Uniprot: P62491
RRID: AB_2838267

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(86%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%), Xenopus(88%)
Clonality:
Polyclonal
Specificity:
RAB11A Antibody detects endogenous levels of total RAB11A.
RRID:
AB_2838267
Cite Format: Affinity Biosciences Cat# DF6301, RRID:AB_2838267.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

MGC1490; Rab 11; Rab 11A; RAB 11A, member oncogene family; Rab-11; RAB11 A; RAB11; Rab11a; RAB11A member RAS oncogene family; Ras related protein Rab 11A; Ras related protein Rab11A; Ras-related protein Rab-11A; RB11A_HUMAN; YL 8; YL8;

Immunogens

Immunogen:

A synthesized peptide derived from human RAB11A, corresponding to a region within C-terminal amino acids.

Uniprot:

P62491(RB11A_HUMAN) >>Visit HPA database.

Gene(ID):
RAB11A(8766)
Description:
Rab11a, Rab11b and Rab25 are members of the Rab11 family of small Ras-like GTPases. Rab11 (isoforms Rab11a and Rab11b) functions as a key regulator in the recycling of perinuclear, plasma membrane and Golgi compartment endosomes (1,2). Despite some overlap, distinct differences exist between Rab11a and Rab11b in both their cellular distribution and functional roles. Rab11a is ubiquitously expressed while Rab11b is found mainly in the heart and brain (3,4). Like other Rab proteins, Rab11 exerts its function via interactions with Rab11 family interacting proteins (FIPs). While there are three distinct classes of FIPs, all appear to share a conserved carboxy-terminal Rab-binding domain that allows Rab-FIP protein interaction. When bound together, these proteins are thought to regulate membrane-associated protein sorting (5,6).
Sequence:
MGTRDDEYDYLFKVVLIGDSGVGKSNLLSRFTRNEFNLESKSTIGVEFATRSIQVDGKTIKAQIWDTAGQERYRAITSAYYRGAVGALLVYDIAKHLTYENVERWLKELRDHADSNIVIMLVGNKSDLRHLRAVPTDEARAFAEKNGLSFIETSALDSTNVEAAFQTILTEIYRIVSQKQMSDRRENDMSPSNNVVPIHVPPTTENKPKVQCCQNI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
88
Chicken
88
Zebrafish
86
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. The small Rab GTPase RAB11A regulates endocytic recycling. Acts as a major regulator of membrane delivery during cytokinesis. Together with MYO5B and RAB8A participates in epithelial cell polarization. Together with RAB3IP, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B participates in CFTR trafficking to the plasma membrane and TF (Transferrin) recycling in nonpolarized cells. Required in a complex with MYO5B and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Participates in the sorting and basolateral transport of CDH1 from the Golgi apparatus to the plasma membrane. Regulates the recycling of FCGRT (receptor of Fc region of monomeric Ig G) to basolateral membranes. May also play a role in melanosome transport and release from melanocytes.

Subcellular Location:

Cell membrane>Lipid-anchor. Recycling endosome membrane>Lipid-anchor. Cleavage furrow. Cytoplasmic vesicle>Phagosome. Cytoplasmic vesicle membrane.
Note: Translocates with RAB11FIP2 from the vesicles of the endocytic recycling compartment (ERC) to the plasma membrane (PubMed:11994279). Localizes to the cleavage furrow (PubMed:15601896). Colocalizes with PARD3, PRKCI, EXOC5, OCLN, PODXL and RAB8A in apical membrane initiation sites (AMIS) during the generation of apical surface and lumenogenesis (PubMed:20890297). Recruited to phagosomes containing S.aureus or M.tuberculosis (PubMed:21255211).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the small GTPase superfamily. Rab family.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Organismal Systems > Excretory system > Endocrine and other factor-regulated calcium reabsorption.

· Organismal Systems > Excretory system > Vasopressin-regulated water reabsorption.

· Organismal Systems > Digestive system > Pancreatic secretion.

References

1). Exocyst controls exosome biogenesis via Rab11a. Molecular Therapy-Nucleic Acids, 2022 (PubMed: 35036064) [IF=6.5]

Application: WB    Species: Human    Sample: HN4 and CNE2 cells

Figure 5 Exocyst trafficks MVBs by interacting with Rab11a (A) Exocyst interacts with Rab11a, as assessed using the STRING database. (B–E) Co-immunoprecipitation (co-IP) assay results showed that Sec10, Sec3, Exo70, and Rab11a interacted in HN4 and CNE2 cells. (F and G) Representative images of immunohistochemical staining (brown) (F) and summary data showing Rab11a expression in LC tissue versus adjacent healthy tissue (Ctrl) (G). ∗p < 0.05 versus siCtrl by t test. Scale bar, 50 μm (H and I) Exosome concentrations after the cells were transfected with scrambled or Rab11a siRNA in HN4 cells. (H) Representative nanoparticle tracking analysis traces. (I) The quantification of exosome concentrations in HN4 cells. ∗∗p < 0.01 versus siCtrl by t test. (J) Representative western blotting images show that CD9, CD63, and CD81 each interact with Rab11a, Exo70, Sec3, and Sec10 in HN4 and CNE2 cells. Lys, whole-cell lysates; Pre-immu, preimmune serum. (K–Z) The interactions between CD63 and Exo70, Sec3, Sec10, or Rab11a after siRNA-mediated of Exo70, Sec3, Sec10, or Rab11a knockdown. CD63 was used for normalization. ∗p < 0.05, ∗∗p < 0.01 versus siCtrl by t test.

Application: IHC    Species: Human    Sample: HN4 and CNE2 cells

Figure 5 Exocyst trafficks MVBs by interacting with Rab11a (A) Exocyst interacts with Rab11a, as assessed using the STRING database. (B–E) Co-immunoprecipitation (co-IP) assay results showed that Sec10, Sec3, Exo70, and Rab11a interacted in HN4 and CNE2 cells. (F and G) Representative images of immunohistochemical staining (brown) (F) and summary data showing Rab11a expression in LC tissue versus adjacent healthy tissue (Ctrl) (G). ∗p < 0.05 versus siCtrl by t test. Scale bar, 50 μm (H and I) Exosome concentrations after the cells were transfected with scrambled or Rab11a siRNA in HN4 cells. (H) Representative nanoparticle tracking analysis traces. (I) The quantification of exosome concentrations in HN4 cells. ∗∗p < 0.01 versus siCtrl by t test. (J) Representative western blotting images show that CD9, CD63, and CD81 each interact with Rab11a, Exo70, Sec3, and Sec10 in HN4 and CNE2 cells. Lys, whole-cell lysates; Pre-immu, preimmune serum. (K–Z) The interactions between CD63 and Exo70, Sec3, Sec10, or Rab11a after siRNA-mediated of Exo70, Sec3, Sec10, or Rab11a knockdown. CD63 was used for normalization. ∗p < 0.05, ∗∗p < 0.01 versus siCtrl by t test.
2). Arf6-mediated macropinocytosis-enhanced suicide gene therapy of C16TAB-condensed Tat/pDNA nanoparticles in ovarian cancer. Nanoscale, 2021 (PubMed: 34473182) [IF=5.8]

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