RHEB Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/3

Western blot analysis of extracts from MCF7, using RHEB Antibody.

DF6299 at 1/100 staining Mouse muscle tissue by IHC-P.

Product:	RHEB Antibody
Catalog:	DF6299
Description:	Rabbit polyclonal antibody to RHEB
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.:	20kDa; 20kD(Calculated).
Uniprot:	Q15382
RRID:	AB_2838265

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(83%)

Clonality:

Polyclonal

Specificity:

RHEB Antibody detects endogenous levels of total RHEB.

RRID:

AB_2838265
Cite Format: Affinity Biosciences Cat# DF6299, RRID:AB_2838265.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Ras homolog enriched in brain 2, formerly; GTP binding protein Rheb; GTP-binding protein Rheb; MGC111559; Ras homolog enriched in brain 2; Ras homolog enriched in brain; RHEB 2; Rheb; RHEB_HUMAN; RHEB2; RHEB2, formerly;

Immunogens

Immunogen:

Uniprot:

Q15382(RHEB_HUMAN) >>Visit HPA database.

Gene(ID):

RHEB(6009)

Expression:

Q15382 RHEB_HUMAN:

Ubiquitous. Highest levels observed in skeletal and cardiac muscle.

Description:

Rheb (Ras Homolog Enriched in Brain) is an evolutionarily conserved member of the Ras family of small GTP-binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure (1). While it is expressed at relatively high levels in the brain, Rheb is widely expressed in other tissues and may be induced by growth factor stimulation. Like other family members, Rheb triggers activation of the Raf-MEK-MAPK pathway (2). Biochemical and genetic studies demonstrate that Rheb has an important role in regulating the insulin/TOR signaling pathway (3-6). The mammalian target of rapamycin, mTOR, is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with translation and cell growth. The tuberin/hamartin (TSC2/TSC1) complex inhibits mTOR activity indirectly by inhibiting Rheb via tuberin's GAP activity (7).

Sequence:

Q15382 RHEB_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPQSKSRKIAILGYRSVGKSSLTIQFVEGQFVDSYDPTIENTFTKLITVNGQEYHLQLVDTAGQDEYSIFPQTYSIDINGYILVYSVTSIKSFEVIKVIHGKLLDMVGKVQIPIMLVGNKKDLHMERVISYEEGKALAESWNAAFLESSAKENQTAVDVFRRIILEAEKMDGAASQGKSSCSVM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Zebrafish

100

Rabbit

100

Xenopus

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15382 As Substrate

Q15382

Site	PTM Type	Enzyme	Source
Y14	Phosphorylation		Uniprot
S16	Phosphorylation		Uniprot
K102	Ubiquitination		Uniprot
K109	Ubiquitination		Uniprot
K120	Ubiquitination		Uniprot
K121	Ubiquitination		Uniprot
S130	Phosphorylation	Q8IW41 (MAPKAPK5)	Uniprot
K135	Ubiquitination		Uniprot
S140	Phosphorylation		Uniprot
K151	Ubiquitination		Uniprot
K169	Ubiquitination		Uniprot
S175	Phosphorylation		Uniprot

PTMs - Q15382 As Enzyme

Q15382

Substrate	Site	Source
P04049-1 (RAF1)	S338	Uniprot
P15056 (BRAF)	S446	Uniprot

Research Backgrounds

Q15382_RHEB_HUMAN

Function:

Activates the protein kinase activity of mTORC1, and thereby plays a role in the regulation of apoptosis. Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Has low intrinsic GTPase activity.

PTMs:

Farnesylation is important for efficiently activating mTORC1-mediated signaling.

Phosphorylation by MAPKAPK5 impairs GTP-binding and inactivation.

Subcellular Location:

Endomembrane system>Lipid-anchor>Cytoplasmic side. Golgi apparatus membrane>Lipid-anchor>Cytoplasmic side. Cytoplasm>Cytosol. Endoplasmic reticulum membrane>Lipid-anchor>Cytoplasmic side.

Tissue Specificity:

Ubiquitous. Highest levels observed in skeletal and cardiac muscle.

Subunit Structure:

Binds to mTORC1 in a guanyl nucleotide-independent manner. Interacts directly with MTOR, MLST8 and RPTOR. Interacts with TSC2. Interacts (when prenylated) with PDE6D; this promotes release from membranes.

Family&Domains:

Belongs to the small GTPase superfamily. Rheb family.

Research Fields

· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Choline metabolism in cancer. (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway. (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway. (View pathway)

References

1). An Injectable Sustained Release Hydrogel of Hyaluronic Acid Loaded with β-Ecdysterone Ameliorates Cartilage Damage in Osteoarthritis via Activating Autophagy. Advanced Therapeutics, 2023 [IF=4.6]

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RHEB Antibody - #DF6299