TPM1 Antibody - #DF6291

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Product: TPM1 Antibody
Catalog: DF6291
Description: Rabbit polyclonal antibody to TPM1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 33kDa; 33kD(Calculated).
Uniprot: P09493
RRID: AB_2838257

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Zebrafish(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%), Xenopus(83%)
Clonality:
Polyclonal
Specificity:
TPM1 Antibody detects endogenous levels of total TPM1.
RRID:
AB_2838257
Cite Format: Affinity Biosciences Cat# DF6291, RRID:AB_2838257.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AA986836; AI854628; Alpha tropomyosin; alpha-TM; Alpha-tropomyosin; C15orf13; cardiomyopathy, hypertrophic 3; CMD1Y; CMH3; HTM alpha; HTM-alpha; OTTHUMP00000163688; sarcomeric tropomyosin kappa; TM2; Tmpa; TMSA; Tpm-1; TPM1; TPM1_HUMAN; tropomyosin 1 (alpha); tropomyosin 1 (alpha) isoform 1; tropomyosin 1 (alpha) isoform 2; tropomyosin 1 (alpha) isoform 3; tropomyosin 1 (alpha) isoform 4; tropomyosin 1 (alpha) isoform 5; tropomyosin 1 (alpha) isoform 6; tropomyosin 1 (alpha) isoform 7; Tropomyosin 1; Tropomyosin alpha 1 chain; Tropomyosin alpha-1 chain; Tropomyosin, skeletal muscle alpha; Tropomyosin-1;

Immunogens

Immunogen:
Uniprot:

P09493(TPM1_HUMAN) >>Visit HPA database.

Gene(ID):
TPM1(7168)
Expression:
P09493 TPM1_HUMAN:

Detected in primary breast cancer tissues but undetectable in normal breast tissues in Sudanese patients. Isoform 1 is expressed in adult and fetal skeletal muscle and cardiac tissues, with higher expression levels in the cardiac tissues. Isoform 10 is expressed in adult and fetal cardiac tissues, but not in skeletal muscle.

Description:
Tropomyosin-1 (TPM1) belongs to the high molecular weight members of tropomyosin family (1,2). The protein exists in an alpha-helical coiled-coil conformation and binds multiple acting monomers in a tight manner to stabilize and regulate the actin filament (3). Tropomyosins fullfill functions in muscle and non-muscle cells. In muscle cells, tropomyosins associate with the troponin complex and play a central role in the calcium-dependent regulation of striated muscle contraction in vertebrates. In non-muscle cells, tropomyosins are implicated in the formation and stabilization of cytoskeletal actin filaments to ensure normal cellular processes (1,2). Mutations of tropomysin-1 have been reported as a cause of dilated cardiac myopathies (4). Tropomyosin-1 also functions as a tumor suppressor, and many malignant tumors demonstrate downregulation of tropomyosin-1 expression (5-8). Tropomyosin-1 is phosphorylated at Ser283 through the Erk/DAPK pathway, which promotes stress fiber formation in response to oxidative stress (9-10).
Sequence:
MDAIKKKMQMLKLDKENALDRAEQAEADKKAAEDRSKQLEDELVSLQKKLKGTEDELDKYSEALKDAQEKLELAEKKATDAEADVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIESRAQKDEEKMEIQEIQLKEAKHIAEDADRKYEEVARKLVIIESDLERAEERAELSEGKCAELEEELKTVTNNLKSLEAQAEKYSQKEDRYEEEIKVLSDKLKEAETRAEFAERSVTKLEKSIDDLEDELYAQKLKYKAISEELDHALNDMTSI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Rabbit
100
Pig
92
Chicken
92
Xenopus
83
Horse
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P09493 As Substrate

Site PTM Type Enzyme
M1 Acetylation
K12 Acetylation
K12 Ubiquitination
K15 Acetylation
K15 Ubiquitination
K29 Acetylation
K29 Ubiquitination
K30 Ubiquitination
K37 Ubiquitination
S45 Phosphorylation
K48 Acetylation
K48 Ubiquitination
T53 Phosphorylation
K59 Ubiquitination
Y60 Phosphorylation
S61 Phosphorylation P42336 (PIK3CA)
K77 Ubiquitination
T79 Phosphorylation
S87 Phosphorylation
T108 Phosphorylation
K112 Acetylation
K112 Ubiquitination
K118 Acetylation
K118 Ubiquitination
S123 Phosphorylation
S132 Phosphorylation
K149 Acetylation
R160 Methylation
K161 Acetylation
K161 Methylation
Y162 Phosphorylation
K168 Ubiquitination
S174 Phosphorylation
T199 Phosphorylation
T201 Phosphorylation
S206 Phosphorylation
K213 Ubiquitination
Y214 Phosphorylation
S215 Phosphorylation
Y221 Phosphorylation
K226 Ubiquitination
S229 Phosphorylation
K231 Ubiquitination
K233 Ubiquitination
T237 Phosphorylation
T247 Phosphorylation
K248 Ubiquitination
S252 Phosphorylation
Y261 Phosphorylation
K264 Ubiquitination
Y267 Phosphorylation
K268 Ubiquitination
S271 Phosphorylation
T282 Phosphorylation
S283 Phosphorylation P53355 (DAPK1)

Research Backgrounds

Function:

Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.

PTMs:

Phosphorylated at Ser-283 by DAPK1 in response to oxidative stress and this phosphorylation enhances stress fiber formation in endothelial cells.

Subcellular Location:

Cytoplasm>Cytoskeleton.
Note: Associates with F-actin stress fibers.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in primary breast cancer tissues but undetectable in normal breast tissues in Sudanese patients. Isoform 1 is expressed in adult and fetal skeletal muscle and cardiac tissues, with higher expression levels in the cardiac tissues. Isoform 10 is expressed in adult and fetal cardiac tissues, but not in skeletal muscle.

Subunit Structure:

Homodimer. Heterodimer of an alpha (TPM1, TPM3 or TPM4) and a beta (TPM2) chain (By similarity). Interacts with HRG (via the HRR domain); the interaction contributes to the antiangiogenic properties of the histidine/proline-rich region (HRR) of HRG (By similarity). Interacts (via N-terminus) with LMOD2 (via N-terminus) and TMOD1 (via N-terminus).

Family&Domains:

The molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity.

Belongs to the tropomyosin family.

Research Fields

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).

· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).

· Organismal Systems > Circulatory system > Cardiac muscle contraction.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

References

1). Allicin induces cell cycle arrest and apoptosis of breast cancer cells in vitro via modulating the p53 pathway. Molecular Biology Reports, 2021 (PubMed: 34626309) [IF=2.8]
2). miR-22 alleviates sepsis-induced acute kidney injury via targeting the HMGB1/TLR4/NF-κB signaling pathway. INTERNATIONAL UROLOGY AND NEPHROLOGY, 2023 (PubMed: 35960478) [IF=2.0]

Application: WB    Species: Rat    Sample: HBZY-1 cells

Fig. 7 miR-22 decreased the expression of TLR4, TLR2, MyD88, and p-NF-κB p65 protein in the AKI vitro model. Western blot was conducted to measure the protein expression of TLR4, TLR2, MyD88, TM and p-NF-κB p65 in HBZY-1 cells. #p < 0.05, ##p < 0.01 vs. Control. *p < 0.05, **p < 0.01 vs. LPS

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