MAD1L1 Antibody - #DF6287

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Product: MAD1L1 Antibody
Catalog: DF6287
Description: Rabbit polyclonal antibody to MAD1L1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Dog
Mol.Wt.: 83kDa; 83kD(Calculated).
Uniprot: Q9Y6D9
RRID: AB_2838253

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Dog(92%)
Clonality:
Polyclonal
Specificity:
MAD1L1 Antibody detects endogenous levels of total MAD1L1.
RRID:
AB_2838253
Cite Format: Affinity Biosciences Cat# DF6287, RRID:AB_2838253.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

hMAD1; HsMAD1; MAD1; MAD1 mitotic arrest deficient like 1 (yeast); MAD1-like protein 1; MAD1L1; MD1L1_HUMAN; Mitotic arrest deficient 1; Mitotic arrest deficient 1-like protein 1; Mitotic checkpoint MAD1 protein homolog; Mitotic spindle assembly checkpoint protein MAD1; PIG9; Tax binding protein 181; Tax-binding protein 181; TP53I9; Tumor protein p53 inducible protein 9; TXBP181;

Immunogens

Immunogen:
Uniprot:

Q9Y6D9(MD1L1_HUMAN) >>Visit HPA database.

Gene(ID):
MAD1L1(8379)
Expression:
Q9Y6D9 MD1L1_HUMAN:

Expressed weakly at G0/G1 and highly at late S and G2/M phase.

Description:
MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Sequence:
MEDLGENTMVLSTLRSLNNFISQRVEGGSGLDISTSAPGSLQMQYQQSMQLEERAEQIRSKSHLIQVEREKMQMELSHKRARVELERAASTSARNYEREVDRNQELLTRIRQLQEREAGAEEKMQEQLERNRQCQQNLDAASKRLREKEDSLAQAGETINALKGRISELQWSVMDQEMRVKRLESEKQELQEQLDLQHKKCQEANQKIQELQASQEARADHEQQIKDLEQKLSLQEQDAAIVKNMKSELVRLPRLERELKQLREESAHLREMRETNGLLQEELEGLQRKLGRQEKMQETLVGLELENERLLAKLQSWERLDQTMGLSIRTPEDLSRFVVELQQRELALKDKNSAVTSSARGLEKARQQLQEELRQVSGQLLEERKKRETHEALARRLQKRVLLLTKERDGMRAILGSYDSELTPAEYSPQLTRRMREAEDMVQKVHSHSAEMEAQLSQALEELGGQKQRADMLEMELKMLKSQSSSAEQSFLFSREEADTLRLKVEELEGERSRLEEEKRMLEAQLERRALQGDYDQSRTKVLHMSLNPTSVARQRLREDHSQLQAECERLRGLLRAMERGGTVPADLEAAAASLPSSKEVAELKKQVESAELKNQRLKEVFQTKIQEFRKACYTLTGYQIDITTENQYRLTSLYAEHPGDCLIFKATSPSGSKMQLLETEFSHTVGELIEVHLRRQDSIPAFLSSLTLELFSRQTVA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
92
Xenopus
75
Chicken
75
Zebrafish
58
Sheep
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y6D9 As Substrate

Site PTM Type Enzyme
M1 Acetylation
T8 Phosphorylation
S12 Phosphorylation
T13 Phosphorylation
S16 Phosphorylation
S22 Phosphorylation P53350 (PLK1)
S29 Phosphorylation P53350 (PLK1)
K61 Acetylation
K61 Sumoylation
K61 Ubiquitination
S62 Phosphorylation
S77 Phosphorylation
K79 Ubiquitination
S90 Phosphorylation
T91 Phosphorylation
S92 Phosphorylation
Y96 Phosphorylation
K123 Ubiquitination
K143 Acetylation
K143 Ubiquitination
K148 Ubiquitination
K163 Ubiquitination
S185 Phosphorylation
K207 Ubiquitination
S214 Phosphorylation Q13315 (ATM)
K231 Ubiquitination
S233 Phosphorylation
K313 Ubiquitination
T323 Phosphorylation
K351 Ubiquitination
S377 Phosphorylation
K406 Ubiquitination
T423 Phosphorylation
Y427 Phosphorylation
S428 Phosphorylation
T432 Phosphorylation
K467 Ubiquitination
K478 Acetylation
K481 Ubiquitination
S484 Phosphorylation
S485 Phosphorylation
S486 Phosphorylation
S490 Phosphorylation
S494 Phosphorylation
T500 Phosphorylation
K504 Ubiquitination
S513 Phosphorylation
Y535 Phosphorylation
S538 Phosphorylation
S551 Phosphorylation
S562 Phosphorylation
T583 Phosphorylation
S597 Phosphorylation
S598 Phosphorylation
K599 Ubiquitination
S610 Phosphorylation
K614 Ubiquitination
K619 Ubiquitination
T624 Phosphorylation
K625 Ubiquitination
Y634 Phosphorylation
T644 Phosphorylation
T680 Phosphorylation P53350 (PLK1)
S699 Phosphorylation
T708 Phosphorylation
T716 Phosphorylation

Research Backgrounds

Function:

Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding.

PTMs:

Phosphorylated; by BUB1. Become hyperphosphorylated in late S through M phases or after mitotic spindle damage.

Subcellular Location:

Nucleus. Chromosome>Centromere>Kinetochore. Nucleus envelope. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Spindle. Cytoplasm>Cytoskeleton>Spindle pole.
Note: Detected at the nucleus envelope during interphase (PubMed:22351768). From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody. Detected at kinetochores during prometaphase (PubMed:22351768). Colocalizes with NEK2 at the kinetochore (PubMed:14978040). Colocalizes with IK at spindle poles during metaphase and anaphase (PubMed:22351768).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed weakly at G0/G1 and highly at late S and G2/M phase.

Subunit Structure:

Homodimer. Heterodimerizes with MAD2L1 in order to form a tetrameric MAD1L1-MAD2L1 core complex. Perturbation of the original MAD1L1-MAD2L1 structure by the spindle checkpoint may decrease MAD2L1 affinity for MAD1L1. CDC20 can compete with MAD1L1 for MAD2L1 binding, until the attachment and/or tension dampen the checkpoint signal, preventing further release of MAD2L1 on to CDC20. Also able to interact with the BUB1/BUB3 complex. Interacts with NEK2. Interacts with TPR; the interactions occurs in a microtubule-independent manner. Interacts with IK. Interacts with the viral Tax protein.

Family&Domains:

Belongs to the MAD1 family.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

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