RRM1 Antibody - #DF6286

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Product: RRM1 Antibody
Catalog: DF6286
Description: Rabbit polyclonal antibody to RRM1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 90kDa; 90kD(Calculated).
Uniprot: P23921
RRID: AB_2838252

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(80%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(90%)
Clonality:
Polyclonal
Specificity:
RRM1 Antibody detects endogenous levels of total RRM1.
RRID:
AB_2838252
Cite Format: Affinity Biosciences Cat# DF6286, RRID:AB_2838252.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

R1; Ribonucleoside diphosphate reductase large subunit; Ribonucleoside diphosphate reductase M1 chain; Ribonucleoside diphosphate reductase subunit M1; Ribonucleoside reductase, large subunit; Ribonucleoside-diphosphate reductase large subunit; Ribonucleoside-diphosphate reductase subunit M1; Ribonucleotide reductase large chain; Ribonucleotide reductase large subunit; Ribonucleotide reductase M1; Ribonucleotide reductase M1 polypeptide; Ribonucleotide reductase R1 subunit; Ribonucleotide reductase, M1 subunit; RIR 1; RIR1; RIR1_HUMAN; RR 1; RR1; RRM 1; RRM1;

Immunogens

Immunogen:
Uniprot:

P23921(RIR1_HUMAN) >>Visit HPA database.

Gene(ID):
RRM1(6240)
Description:
Ribonucleotide reductase catalyzes the rate-limiting step in the synthesis of deoxynucleotide triphosphates (dNTPs). The regulatory M1 subunit (RRM1) is present throughout the cell division cycle, but downregulated in quiescent cells (1). Research studies have demonstrated that RRM1 is involved in carcinogenesis and tumor progression, and its expression is correlated with resistance to chemotherapy in non-small cell lung cancer (2-4).
Sequence:
MHVIKRDGRQERVMFDKITSRIQKLCYGLNMDFVDPAQITMKVIQGLYSGVTTVELDTLAAETAATLTTKHPDYAILAARIAVSNLHKETKKVFSDVMEDLYNYINPHNGKHSPMVAKSTLDIVLANKDRLNSAIIYDRDFSYNYFGFKTLERSYLLKINGKVAERPQHMLMRVSVGIHKEDIDAAIETYNLLSERWFTHASPTLFNAGTNRPQLSSCFLLSMKDDSIEGIYDTLKQCALISKSAGGIGVAVSCIRATGSYIAGTNGNSNGLVPMLRVYNNTARYVDQGGNKRPGAFAIYLEPWHLDIFEFLDLKKNTGKEEQRARDLFFALWIPDLFMKRVETNQDWSLMCPNECPGLDEVWGEEFEKLYASYEKQGRVRKVVKAQQLWYAIIESQTETGTPYMLYKDSCNRKSNQQNLGTIKCSNLCTEIVEYTSKDEVAVCNLASLALNMYVTSEHTYDFKKLAEVTKVVVRNLNKIIDINYYPVPEACLSNKRHRPIGIGVQGLADAFILMRYPFESAEAQLLNKQIFETIYYGALEASCDLAKEQGPYETYEGSPVSKGILQYDMWNVTPTDLWDWKVLKEKIAKYGIRNSLLIAPMPTASTAQILGNNESIEPYTSNIYTRRVLSGEFQIVNPHLLKDLTERGLWHEEMKNQIIACNGSIQSIPEIPDDLKQLYKTVWEISQKTVLKMAAERGAFIDQSQSLNIHIAEPNYGKLTSMHFYGWKQGLKTGMYYLRTRPAANPIQFTLNKEKLKDKEKVSKEEEEKERNTAAMVCSLENRDECLMCGS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
90
Zebrafish
80
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P23921 As Substrate

Site PTM Type Enzyme
K5 Ubiquitination
K17 Acetylation
K17 Ubiquitination
K24 Ubiquitination
K70 Ubiquitination
Y74 Phosphorylation
R80 Methylation
K88 Ubiquitination
K92 Ubiquitination
Y102 Phosphorylation
Y104 Phosphorylation
K111 Ubiquitination
K118 Ubiquitination
S119 Phosphorylation
K128 Ubiquitination
S133 Phosphorylation
S142 Phosphorylation
K149 Ubiquitination
K158 Ubiquitination
K162 Ubiquitination
K180 Ubiquitination
Y232 Phosphorylation
K236 Ubiquitination
K243 Ubiquitination
S253 Phosphorylation
K320 Methylation
K320 Ubiquitination
K369 Ubiquitination
K376 Acetylation
K376 Ubiquitination
K414 Ubiquitination
S415 Phosphorylation
T422 Phosphorylation
K424 Ubiquitination
K464 Ubiquitination
K465 Ubiquitination
K471 Ubiquitination
K479 Ubiquitination
Y485 Phosphorylation
Y486 Phosphorylation
K496 Acetylation
K496 Ubiquitination
K548 Ubiquitination
Y553 Phosphorylation
T555 Phosphorylation
Y556 Phosphorylation
S559 Phosphorylation
K563 Ubiquitination
K582 Ubiquitination
K587 Ubiquitination
K590 Ubiquitination
T604 Phosphorylation
S631 Phosphorylation
K643 Ubiquitination
K656 Ubiquitination
K677 Ubiquitination
K681 Ubiquitination
K689 Ubiquitination
K693 Ubiquitination
Y726 Phosphorylation
K729 Ubiquitination
K733 Ubiquitination
Y738 Phosphorylation
T751 Phosphorylation
K754 Ubiquitination
K762 Ubiquitination
K765 Ubiquitination
K770 Ubiquitination
T774 Phosphorylation
S780 Phosphorylation
S792 Phosphorylation

Research Backgrounds

Function:

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Heterodimer of a large and a small subunit. Heterodimer with small subunit RRM2 or RRM2B. The heterodimer with RRM2 has higher catalytic activity than the heterodimer with RRM2B. Interacts with AHCYL1 which inhibits its activity.

Family&Domains:

Belongs to the ribonucleoside diphosphate reductase large chain family.

Research Fields

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

· Metabolism > Metabolism of other amino acids > Glutathione metabolism.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Gambogic acid sensitizes gemcitabine efficacy in pancreatic cancer by reducing the expression of ribonucleotide reductase subunit-M2 (RRM2). Journal of Experimental & Clinical Cancer Research, 2017 (PubMed: 28797284) [IF=11.3]

Application: WB    Species: human    Sample:

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