Product: Ferritin Heavy Chain Antibody
Catalog: DF6278
Description: Rabbit polyclonal antibody to Ferritin Heavy Chain
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 21kDa; 21kD(Calculated).
Uniprot: P02794
RRID: AB_2838244

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Rabbit(90%), Dog(90%), Chicken(80%), Xenopus(88%)
Clonality:
Polyclonal
Specificity:
Ferritin Heavy Chain Antibody detects endogenous levels of total Ferritin Heavy Chain.
RRID:
AB_2838244
Cite Format: Affinity Biosciences Cat# DF6278, RRID:AB_2838244.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Apoferritin; Cell proliferation inducing gene 15 protein; Cell proliferation-inducing gene 15 protein; F HC; Ferritin H subunit; Ferritin heavy chain; Ferritin heavy polypeptide 1; FHC; FRIH; FRIH_HUMAN; FTH 1; FTH; FTH1; FTH1 protein; FTHL 6; FTHL6; Iron overload autosomal dominant; MGC104426; N-terminally processed; OK/SW-cl.84; PIG 15; PIG15; Placenta immunoregulatory factor; PLIF; Proliferation inducing gene 15 protein; Proliferation inducing protein 15;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P02794 FRIH_HUMAN:

Expressed in the liver.

Description:
Ferritin (FTH) is a ubiquitous and highly conserved protein which plays a major role in iron homeostasis by sequestering and storing iron in a non-toxic and bioavailable form (1). The assembled ferritin molecule, often referred to as a nanocage, can store up to 4,500 atoms of iron (2,3). It forms a holoenzyme of ~450 kDa, consisting of 24 subunits made up of two types of polypeptide chains: ferritin heavy chain and ferritin light chain, each having unique functions. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe(II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe(III) (4). In addition to iron buffering, heavy chain ferritin also enhances thymidine biosynthesis (5). Serum ferritin levels serve as an indicator of the amount of iron stored in the body. Serum ferritin is the most sensitive test for anaemia. The level of serum ferritin is markedly elevated in inflammation, malignancy, and iron overload disorders (6). Research studies have found that defects in ferritin proteins are also associated with several neurodegenerative diseases (7).
Sequence:
MTTASTSQVRQNYHQDSEAAINRQINLELYASYVYLSMSYYFDRDDVALKNFAKYFLHQSHEEREHAEKLMKLQNQRGGRIFLQDIKKPDCDDWESGLNAMECALHLEKNVNQSLLELHKLATDKNDPHLCDFIETHYLNEQVKAIKELGDHVTNLRKMGAPESGLAEYLFDKHTLGDSDNES

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
90
Dog
90
Xenopus
88
Chicken
80
Horse
78
Pig
70
Bovine
70
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P02794 As Substrate

Site PTM Type Enzyme
M1 Acetylation
T2 Acetylation
T6 Phosphorylation
S7 Phosphorylation
Y13 Phosphorylation
K54 Ubiquitination
K69 Ubiquitination
K72 Sumoylation
K72 Ubiquitination
K88 Ubiquitination
S96 Phosphorylation
K109 Ubiquitination
S114 Phosphorylation
K120 Ubiquitination
K125 Ubiquitination
K144 Ubiquitination
K147 Ubiquitination
K158 Ubiquitination
S164 Phosphorylation
Y169 Phosphorylation
T175 Phosphorylation
S179 Phosphorylation
S183 Phosphorylation

Research Backgrounds

Function:

Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity).

Tissue Specificity:

Expressed in the liver.

Subunit Structure:

Oligomer of 24 subunits. There are two types of subunits: L (light) chain and H (heavy) chain. The major chain can be light or heavy, depending on the species and tissue type. In the human liver, the heavy chain is predominant. The functional molecule forms a roughly spherical shell with a diameter of 12 nm and contains a central cavity into which the insoluble mineral iron core is deposited.

Family&Domains:

Belongs to the ferritin family.

Research Fields

· Cellular Processes > Cell growth and death > Ferroptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Organismal Systems > Digestive system > Mineral absorption.

References

1). Activation of integrated stress response and disordered iron homeostasis upon combined exposure to cadmium and PCB77. JOURNAL OF HAZARDOUS MATERIALS (PubMed: 31837937) [IF=13.6]

Application: WB    Species: Human    Sample: Human erythroleukemia cell lines (HEL)

Fig. 5. Disordered iron homeostasis and inhibited mTORC1 activity upon exposure to CdCl2 and PCB77 at low dose. (A) The relative fluorescence intensity of CAeAM for measuring LIP to reflect intracellular iron availability (n = 3–4), and (B) Representative blots of FTH1 protein content to reflect iron storage. Analyses were performed after single or combined exposure to CdCl2 and PCB77 at 1 μM for 48 h. (C) Phosphorylated S6 and total S6 content to re- flect mTORC1 activity as measured by Western blot. Ratio of FTH1 to eIF2αP and ratio of pS6 to S6 in the control group were defined as 1. Analyses were performed after single or combined exposure to CdCl2 and PCB77 at 1 μM for 48 h. a- significantly different from the control group. Data were presented in mean ± SE. P < 0.05 was considered statistically significant.

2). Oxoeicosanoid receptor inhibition alleviates acute myocardial infarction through activation of BCAT1. BASIC RESEARCH IN CARDIOLOGY (PubMed: 33484341) [IF=9.5]

Application: IHC    Species: mouse    Sample: heart

Fig. 5| Overexpression of BCAT1 and BCAT2 ameliorated AMIinduced myocardial injury. All mice were killed 24 h after the left anterior descending CAL. The heart-specifc BCAT1 or BCAT2 over-expression mice were constructed by direct injecting Adenoassociated virus (AAV) vectors carrying BCAT1 or BCAT2 in the LV free wall in mice using a syringe with a 30-gauge needle.h Representative immunohistochemical analysis of the expression of FHC, FLC, GPX4 and Caspase-3 in heart tissue of sham and model mice with cardiac overexpression of BCAT1 and BCAT2 (n = 3 mice) (400 ×  magnifcation, scale bar, 50 µm). #p < 0.05, ##p < 0.01 versus sham group, *p < 0.05, **p < 0.01 versus model group

3). Tiliroside targets TBK1 to induce ferroptosis and sensitize hepatocellular carcinoma to sorafenib. Phytomedicine (PubMed: 36657316) [IF=7.9]

4). GSK-3β-dependent Nrf2 antioxidant response modulates ferroptosis of lens epithelial cells in age-related cataract. Free Radical Biology and Medicine (PubMed: 37156294) [IF=7.4]

5). YAP1 alleviates sepsis-induced acute lung injury via inhibiting ferritinophagy-mediated ferroptosis. Frontiers in Immunology (PubMed: 35979359) [IF=7.3]

6). YAP1 protects against PM2.5-induced lung toxicity by suppressing pyroptosis and ferroptosis. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (PubMed: 36863160) [IF=6.8]

Application: WB    Species: Mouse    Sample: lung tissues

Fig. 3. YAP1 knockout accelerates ferroptosis in PM2.5-induced lung toxicity. A, Representative fluorescent images of ROS staining; B, Quantification of ROS labeling intensity in the lung; C, Representative TEM images. The red arrow indicates representative mitochondria in WT or YAP1-CKO mouse lungs exposed to PM2.5 or not; D, Immunoblotting detection of SLC7A11, GPX4 and FTH1 expression in mice with the indicated treatment; quantitative results are shown in E-G n = 5; lung content of Fe2+ (H), MDA (I) and GSH (J) measured by the corresponding commercial kit, n = 5. The values are shown as the mean ± SD,

7). Withaferin A inhibits ferroptosis and protects against intracerebral hemorrhage. Neural Regeneration Research (PubMed: 36453416) [IF=6.1]

Application: WB    Species: Mouse    Sample:

Figure 8 WFA induces HO-1 expression, resulting in ameliorated ferroptosis in hemin-induced SH-SY5Y neuroblast injury in vitro. (A) Representative western blot bands and (B) relative density of HO-1 in different groups. (C) Cell viability detection in different groups. (D) Typical BODIPY 581/591 C11 fluorescence images and (E) fluorescence density of BODIPY 581/591 C11 in different groups. Green indicates lipid ROS and blue indicates nuclei. (F) Representative western blot bands and (G–I) quantification of the relative density of 4-HNE, TF and FTH1 band densities in different groups. *P < 0.05, **P < 0.01 (one-way analysis of variance followed by Bonferroni correction). Data are expressed as the mean ± SEM (n = 3). 4-HNE: 4-Hydroxynonenal; FTH1: ferritin heavy chain 1; HO-1: heme oxygenase-1; TF: transferrin; WFA: withaferin A; ZnPP: zinc protoporphyrin, a specific HO-1 inhibitor.

8). Patulin Induces Acute Kidney Injury in Mice through Autophagy–Ferroptosis Pathway. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (PubMed: 35543324) [IF=6.1]

9). Erythropoietin inhibits ferroptosis and ameliorates neurological function after spinal cord injury. Neural Regeneration Research (PubMed: 36204858) [IF=6.1]

Application: WB    Species: Rat    Sample: spinal cord

Figure 3 EPO regulates the expression of ferroptotic biomarkers after SCI. (A) Western blot of the indicated proteins in injured tissues from the treatment groups. (B–H) Quantitative analysis of Tfr, Fpn, Fth, Acsl4 and 4-Hne protein expression at 14 dpi. Gapdh was used as the reference protein. (I, J) Quantitative reverse transcription polymerase chain reaction results of xCT and Gpx4 mRNA extracted from injured tissue at 7 dpi. β-Actin mRNA was used as the reference gene. (K) Quantitative analysis of reduced GSH from injured tissue at 7 dpi. All data are expressed as the mean ± SD (n = 5 in each group). *P < 0.05, **P < 0.01, ***P < 0.001 (one-way analysis of variance followed by Dunnett’s multiple comparisons test). 4-Hne: 4-Hydroxynonenal; Acsl4: acyl-coenzyme A synthetase long chain family member 4; dpi: day(s) post injury; EPO: erythropoietin; Fpn: ferroportin or solute carrier family 40 member 1; Fth: ferritin heavy chain; Gapdh: glyceraldehyde-3-phosphate dehydrogenase; Gpx4: glutathione peroxidase 4; GSH: glutathione; ns: not significant; SCI: spinal cord injury; Tfr: transferrin receptor; xCT: the solute carrier family 7 member 11.

10). Melatonin suppresses ferroptosis via activation of the Nrf2/HO-1 signaling pathway in the mouse model of sepsis-induced acute kidney injury. International Immunopharmacology (PubMed: 36067654) [IF=5.6]

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