RALBP1 Antibody - #DF6274

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Product: RALBP1 Antibody
Catalog: DF6274
Description: Rabbit polyclonal antibody to RALBP1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 95kDa; 76kD(Calculated).
Uniprot: Q15311
RRID: AB_2838240

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(88%), Bovine(88%), Horse(88%), Rabbit(100%), Dog(88%), Chicken(88%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
RALBP1 Antibody detects endogenous levels of total RALBP1.
RRID:
AB_2838240
Cite Format: Affinity Biosciences Cat# DF6274, RRID:AB_2838240.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

RLIP1; 76 kDa Ral-interacting protein; 76-kDa Ral-interacting protein; Dinitrophenyl S-glutathione ATPase; DNP-SG ATPase; Ral-interacting protein 1; Ral-interacting protein 1, 76-KD; RalA-binding protein 1; RalBP1; RBP1_HUMAN; RIP1; RLIP1; RLIP76;

Immunogens

Immunogen:
Uniprot:

Q15311(RBP1_HUMAN) >>Visit HPA database.

Gene(ID):
RALBP1(10928)
Expression:
Q15311 RBP1_HUMAN:

Expressed ubiquitously but at low levels. Shows a strong expression in the erythrocytes.

Description:
The RalA binding protein 1 (RalBP1 or RLIP76) was originally identified as a GTP-RalA associated protein that acted as a downstream RalA effector in regulating Ral-Ras signaling (1). RalBP1 interacts with RalA and the endocytosis protein REPS2 (POB1) through its carboxy-terminal Ral binding domain. RalBP1 has an intrinsic GTPase activating function and interacts with Cdc42 through its centrally located Rho-GAP domain (1-3). A protein complex containing RalBP1/POB1/RalA regulates endocytosis of membrane receptors (4). RalBP1 also functions as a non-ABC transporter that catalyzes the ATP-dependent transport of numerous xenobiotics, including glutathione conjugates and some chemotherapeutic agents. RalBP1 transporter activity may play an important role in detoxification, drug resistance and the stress response (5-7). Increased expression of RalBP1 protein is associated with some forms of cancer and regression of cancer xenografts results from RalBP1 inhibition (8,9). Evidence to date suggests that RalBP1 may be a promising therapeutic target for cancer therapy.
Sequence:
MTECFLPPTSSPSEHRRVEHGSGLTRTPSSEEISPTKFPGLYRTGEPSPPHDILHEPPDVVSDDEKDHGKKKGKFKKKEKRTEGYAAFQEDSSGDEAESPSKMKRSKGIHVFKKPSFSKKKEKDFKIKEKPKEEKHKEEKHKEEKHKEKKSKDLTAADVVKQWKEKKKKKKPIQEPEVPQIDVPNLKPIFGIPLADAVERTMMYDGIRLPAVFRECIDYVEKYGMKCEGIYRVSGIKSKVDELKAAYDREESTNLEDYEPNTVASLLKQYLRDLPENLLTKELMPRFEEACGRTTETEKVQEFQRLLKELPECNYLLISWLIVHMDHVIAKELETKMNIQNISIVLSPTVQISNRVLYVFFTHVQELFGNVVLKQVMKPLRWSNMATMPTLPETQAGIKEEIRRQEFLLNCLHRDLQGGIKDLSKEERLWEVQRILTALKRKLREAKRQECETKIAQEIASLSKEDVSKEEMNENEEVINILLAQENEILTEQEELLAMEQFLRRQIASEKEEIERLRAEIAEIQSRQQHGRSETEEYSSESESESEDEEELQIILEDLQRQNEELEIKNNHLNQAIHEEREAIIELRVQLRLLQMQRAKAEQQAQEDEEPEWRGGAVQPPRDGVLEPKAAKEQPKAGKEPAKPSPSRDRKETSI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Xenopus
100
Pig
88
Horse
88
Bovine
88
Dog
88
Chicken
88
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15311 As Substrate

Site PTM Type Enzyme
T2 Acetylation
T9 Phosphorylation
S11 Phosphorylation
S13 Phosphorylation
T25 Phosphorylation
T27 Phosphorylation
S29 Phosphorylation
S30 Phosphorylation
S34 Phosphorylation
T36 Phosphorylation
T44 Phosphorylation
S48 Phosphorylation
S62 Phosphorylation
T82 Phosphorylation
Y85 Phosphorylation
S92 Phosphorylation
S93 Phosphorylation
S99 Phosphorylation
S116 Phosphorylation
S118 Phosphorylation P17252 (PRKCA)
S151 Phosphorylation
K152 Ubiquitination
K161 Ubiquitination
Y219 Phosphorylation
S234 Phosphorylation
S252 Phosphorylation
T253 Phosphorylation
K268 Ubiquitination
Y270 Phosphorylation
T280 Phosphorylation
K281 Ubiquitination
T297 Phosphorylation P17252 (PRKCA)
K299 Ubiquitination
S353 Phosphorylation P17252 (PRKCA)
K421 Ubiquitination
K454 Ubiquitination
S461 Phosphorylation
S463 Phosphorylation
K469 Ubiquitination
S509 Phosphorylation P17252 (PRKCA)
K600 Ubiquitination
S645 Phosphorylation
S647 Phosphorylation

Research Backgrounds

Function:

Can activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. Can catalyze transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon. Serves as a scaffold protein that brings together proteins forming an endocytotic complex during interphase and also with CDK1 to switch off endocytosis, One of its substrates would be EPN1/Epsin.

Subcellular Location:

Membrane>Peripheral membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed ubiquitously but at low levels. Shows a strong expression in the erythrocytes.

Subunit Structure:

Interacts with the GTP-bound form of RALA, RALB, CDC42 and RAC1. Interacts with REPS1 and REPS2 and this does not affect the Ral-binding activity. Interacts with DAB2IP. Interacts with catalytically active CCNB1 and CDK1 during mitosis. Interacts with EPN1, NUMB and TFAP2A during interphase and mitosis.

Research Fields

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

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