MCM7 Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/4

Western blot analysis of extracts from Mouse kidney, using MCM7 Antibody.

DF6272 at 1/100 staining Rat liver tissue by IHC-P.

DF6272 at 1/100 staining Rat brain tissue by IHC-P.

DF6272 staining HepG2 cells by IF/ICC.

Product:	MCM7 Antibody
Catalog:	DF6272
Description:	Rabbit polyclonal antibody to MCM7
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Zebrafish, Bovine, Rabbit, Dog, Xenopus
Mol.Wt.:	81kDa; 81kD(Calculated).
Uniprot:	P33993
RRID:	AB_2838238

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Zebrafish(100%), Bovine(100%), Rabbit(100%), Dog(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

MCM7 Antibody detects endogenous levels of total MCM7.

RRID:

AB_2838238
Cite Format: Affinity Biosciences Cat# DF6272, RRID:AB_2838238.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CDABP0042; CDC 47; CDC47; CDC47 homolog; Cdc47, S. cerevisiae, homolog of; DNA replication licensing factor MCM7; Homolog of S. cerevisiae Cdc47; MCM 2; MCM 7; MCM2; MCM2, formerly; Mcm7; MCM7 minichromosome maintenance deficient 7; MCM7_HUMAN; Minichromosome Maintainence 7; Minichromosome maintainence, S. cerevisiae, homolog of; Minichromosome maintenance complex component 7; Minichromosome maintenance deficient 7; Minichromosome maintenance protein 7; P1.1 MCM3; P1.1-MCM3; P1CDC47; P85MCM; PNAS 146; PNAS146; PPP1R104; Protein phosphatase 1, regulatory subunit 104;

Immunogens

Immunogen:

Uniprot:

P33993(MCM7_HUMAN) >>Visit HPA database.

Gene(ID):

MCM7(4176)

Description:

The minichromosome maintenance (MCM) 2-7 proteins are a family of six related proteins required for the initiation and elongation of DNA replication. MCM2-7 bind together to form the heterohexameric MCM complex that is thought to act as a replicative helicase at the DNA replication fork (1-5). This complex is also a key component of the pre-replication complex (pre-RC) (reviewed in 1). Cdc6 and CDT1 recruit the MCM complex to the origin recognition complex (ORC) during late mitosis/early G1 phase forming the pre-RC and licensing the DNA for replication (reviewed in 2). Phosphorylation of the MCM2, MCM3, MCM4, and MCM6 subunits appears to regulate MCM complex activity and the initiation of DNA synthesis (6-8). MCM proteins are removed during DNA replication, causing chromatin to become unlicensed through inhibition of pre-RC reformation. Licensing of the chromatin permits the DNA to replicate only once per cell cycle, thereby helping to ensure that genetic alterations and malignant cell growth do not occur (reviewed in 3). Studies have shown that the MCM complex is involved in checkpoint control by protecting the structure of the replication fork and assisting in restarting replication by recruiting checkpoint proteins after arrest (reviewed in 3,9).

Sequence:

P33993 MCM7_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MALKDYALEKEKVKKFLQEFYQDDELGKKQFKYGNQLVRLAHREQVALYVDLDDVAEDDPELVDSICENARRYAKLFADAVQELLPQYKEREVVNKDVLDVYIEHRLMMEQRSRDPGMVRSPQNQYPAELMRRFELYFQGPSSNKPRVIREVRADSVGKLVTVRGIVTRVSEVKPKMVVATYTCDQCGAETYQPIQSPTFMPLIMCPSQECQTNRSGGRLYLQTRGSRFIKFQEMKMQEHSDQVPVGNIPRSITVLVEGENTRIAQPGDHVSVTGIFLPILRTGFRQVVQGLLSETYLEAHRIVKMNKSEDDESGAGELTREELRQIAEEDFYEKLAASIAPEIYGHEDVKKALLLLLVGGVDQSPRGMKIRGNINICLMGDPGVAKSQLLSYIDRLAPRSQYTTGRGSSGVGLTAAVLRDSVSGELTLEGGALVLADQGVCCIDEFDKMAEADRTAIHEVMEQQTISIAKAGILTTLNARCSILAAANPAYGRYNPRRSLEQNIQLPAALLSRFDLLWLIQDRPDRDNDLRLAQHITYVHQHSRQPPSQFEPLDMKLMRRYIAMCREKQPMVPESLADYITAAYVEMRREAWASKDATYTSARTLLAILRLSTALARLRMVDVVEKEDVNEAIRLMEMSKDSLLGDKGQTARTQRPADVIFATVRELVSGGRSVRFSEAEQRCVSRGFTPAQFQAALDEYEELNVWQVNASRTRITFV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

100

Dog

100

Xenopus

100

Zebrafish

100

Rabbit

100

Sheep

Pig

Horse

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P33993 As Substrate

P33993

Site	PTM Type	Enzyme	Source
A2	Acetylation		Uniprot
K4	Sumoylation		Uniprot
K4	Ubiquitination		Uniprot
Y6	Phosphorylation		Uniprot
K10	Sumoylation		Uniprot
K10	Ubiquitination		Uniprot
K15	Sumoylation		Uniprot
K15	Ubiquitination		Uniprot
Y21	Phosphorylation		Uniprot
K28	Acetylation		Uniprot
K28	Ubiquitination		Uniprot
K29	Acetylation		Uniprot
K29	Ubiquitination		Uniprot
K32	Sumoylation		Uniprot
K32	Ubiquitination		Uniprot
K75	Ubiquitination		Uniprot
K89	Ubiquitination		Uniprot
K96	Ubiquitination		Uniprot
Y102	Phosphorylation		Uniprot
S113	Phosphorylation		Uniprot
S121	Phosphorylation	P06493 (CDK1) , P24941 (CDK2)	Uniprot
K145	Ubiquitination		Uniprot
S156	Phosphorylation		Uniprot
K159	Sumoylation		Uniprot
K159	Ubiquitination		Uniprot
T168	Phosphorylation		Uniprot
K174	Sumoylation		Uniprot
K174	Ubiquitination		Uniprot
R219	Methylation		Uniprot
R225	Methylation		Uniprot
K231	Sumoylation		Uniprot
K231	Ubiquitination		Uniprot
K236	Sumoylation		Uniprot
K236	Ubiquitination		Uniprot
Y297	Phosphorylation		Uniprot
K305	Ubiquitination		Uniprot
K308	Sumoylation		Uniprot
K308	Ubiquitination		Uniprot
S309	Phosphorylation		Uniprot
S314	Phosphorylation		Uniprot
T320	Phosphorylation		Uniprot
R321	Methylation		Uniprot
R325	Methylation		Uniprot
Y333	Phosphorylation		Uniprot
K335	Ubiquitination		Uniprot
K351	Ubiquitination		Uniprot
K352	Ubiquitination		Uniprot
S365	Phosphorylation	P24941 (CDK2)	Uniprot
K387	Ubiquitination		Uniprot
S388	Phosphorylation		Uniprot
S392	Phosphorylation		Uniprot
R396	Methylation		Uniprot
R407	Methylation		Uniprot
K449	Methylation		Uniprot
K471	Ubiquitination		Uniprot
C482	S-Nitrosylation		Uniprot
Y492	Phosphorylation		Uniprot
S500	Phosphorylation		Uniprot
R524	Methylation		Uniprot
R527	Methylation		Uniprot
R532	Methylation		Uniprot
T538	Phosphorylation		Uniprot
Y539	Phosphorylation		Uniprot
S544	Phosphorylation		Uniprot
S549	Phosphorylation		Uniprot
K557	Ubiquitination		Uniprot
K569	Ubiquitination		Uniprot
K596	Ubiquitination		Uniprot
T599	Phosphorylation		Uniprot
Y600	Phosphorylation	P07948 (LYN)	Uniprot
T605	Phosphorylation		Uniprot
K627	Ubiquitination		Uniprot
K641	Ubiquitination		Uniprot
K648	Ubiquitination		Uniprot
S670	Phosphorylation		Uniprot
R673	Methylation		Uniprot
S674	Phosphorylation		Uniprot
S678	Phosphorylation		Uniprot

Research Backgrounds

P33993_MCM7_HUMAN

Function:

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.

PTMs:

O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.

Subcellular Location:

Nucleus.

Subunit Structure:

Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5 (Probable). Interacts with the ATR-ATRIP complex and with RAD17. Interacts with TIPIN. Interacts with MCMBP. Interacts with ANKRD17. Component of the replisome complex composed of at least DONSON, MCM2, MCM7, PCNA and TICRR.

Family&Domains:

Belongs to the MCM family.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)

· Genetic Information Processing > Replication and repair > DNA replication.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

MCM7 Antibody - #DF6272