FBL Antibody | Affinity Biosciences

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Western blot analysis of extracts from various samples, using FBL Antibody.

Product:	FBL Antibody
Catalog:	DF6270
Description:	Rabbit polyclonal antibody to FBL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	34kDa; 34kD(Calculated).
Uniprot:	P22087
RRID:	AB_2838236

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(92%), Dog(100%)

Clonality:

Polyclonal

Specificity:

FBL Antibody detects endogenous levels of total FBL.

RRID:

AB_2838236
Cite Format: Affinity Biosciences Cat# DF6270, RRID:AB_2838236.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

34 kD nucleolar scleroderma antigen; 34 kDa nucleolar scleroderma antigen; Fbl; FBRL_HUMAN; FIB; FIB1; FLRN; Histone-glutamine methyltransferase; Nop1p; RNA U3 small nucleolar interacting protein 1; RNU3IP1; rRNA 2' O methyltransferase fibrillarin; rRNA 2'-O-methyltransferase fibrillarin;

Immunogens

Immunogen:

Uniprot:

P22087(FBRL_HUMAN) >>Visit HPA database.

Gene(ID):

FBL(2091)

Description:

Fibrillarin is a 2'-O-methyltransferase located in fibrillar regions and Cajal bodies of the nucleolus, where RNA transcription and pre-RNA processing take place (1,2). Fibrillarin associates with several other structual proteins as well as box C/D snoRNA to form a complex that functions in pre-rRNA processing, pre-rRNA methylation and ribosome assembly. This complex catalyzes site-specific 2'-O-ribose methylation of targeted nucleotides within the rRNA sequence (3,4). The sequence, structure and function of fibrillarin are highly conserved and fibrillarin gene expression is essential for early embryonic development (5).

Sequence:

P22087 FBRL_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKPGFSPRGGGFGGRGGFGDRGGRGGRGGFGGGRGRGGGFRGRGRGGGGGGGGGGGGGRGGGGFHSGGNRGRGRGGKRGNQSGKNVMVEPHRHEGVFICRGKEDALVTKNLVPGESVYGEKRVSISEGDDKIEYRAWNPFRSKLAAAILGGVDQIHIKPGAKVLYLGAASGTTVSHVSDIVGPDGLVYAVEFSHRSGRDLINLAKKRTNIIPVIEDARHPHKYRMLIAMVDVIFADVAQPDQTRIVALNAHTFLRNGGHFVISIKANCIDSTASAEAVFASEVKKMQQENMKPQEQLTLEPYERDHAVVVGVYRPPPKVKN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

Zebrafish

Xenopus

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P22087 As Substrate

P22087

Site	PTM Type	Source
S6	Phosphorylation	Uniprot
R8	Methylation	Uniprot
R15	Methylation	Uniprot
R21	Methylation	Uniprot
R24	Methylation	Uniprot
R27	Methylation	Uniprot
R34	Methylation	Uniprot
R36	Methylation	Uniprot
R45	Methylation	Uniprot
R59	Methylation	Uniprot
R70	Methylation	Uniprot
R72	Methylation	Uniprot
R78	Methylation	Uniprot
C99	S-Nitrosylation	Uniprot
K102	Acetylation	Uniprot
K102	Sumoylation	Uniprot
K102	Ubiquitination	Uniprot
K109	Sumoylation	Uniprot
K109	Ubiquitination	Uniprot
S116	Phosphorylation	Uniprot
Y118	Phosphorylation	Uniprot
K121	Acetylation	Uniprot
K121	Ubiquitination	Uniprot
S124	Phosphorylation	Uniprot
S126	Phosphorylation	Uniprot
K131	Ubiquitination	Uniprot
K143	Sumoylation	Uniprot
K143	Ubiquitination	Uniprot
K158	Sumoylation	Uniprot
K158	Ubiquitination	Uniprot
K205	Acetylation	Uniprot
K205	Ubiquitination	Uniprot
K206	Acetylation	Uniprot
K206	Ubiquitination	Uniprot
T208	Phosphorylation	Uniprot
K222	Ubiquitination	Uniprot
K284	Ubiquitination	Uniprot
K285	Ubiquitination	Uniprot
K292	Ubiquitination	Uniprot
T298	Phosphorylation	Uniprot
Y302	Phosphorylation	Uniprot
K318	Ubiquitination	Uniprot

Research Backgrounds

P22087_FBRL_HUMAN

Function:

S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate (By similarity). Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA (By similarity). Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus.

PTMs:

Ubiquitinated. Ubiquitination leads to proteasomal degradation. Deubiquitinated by USP36.

By homology to other fibrillarins, some or all of the N-terminal domain arginines are modified to asymmetric dimethylarginine (DMA).

Acetylated by CREBBP/CBP, preventing methylation of 'Gln-105' of histone H2A (H2AQ104me), without affecting rRNA methylation. Deacetylation by SIRT7 restores methylation of 'Gln-105' of histone H2A (H2AQ104me).

Subcellular Location:

Nucleus>Nucleolus.
Note: Fibrillar region of the nucleolus.

Subunit Structure:

Interacts with NOLC1 (By similarity). Component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles that contain SNU13, FBL, NOP5 and NOP56, plus a guide RNA. It is associated with the U3, U8, U13, X and Y small nuclear RNAs. Component of several ribosomal and nucleolar protein complexes. Interacts with PRMT5 and UTP20. Interacts with DDX5 and C1QBP. Interacts with NOL11. Interacts with PIH1D1. Interacts with RRP1B.

Family&Domains:

Belongs to the methyltransferase superfamily. Fibrillarin family.

Research Fields

· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.

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FBL Antibody - #DF6270