Emerin Antibody - #DF6266

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Product: Emerin Antibody
Catalog: DF6266
Description: Rabbit polyclonal antibody to Emerin
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Rabbit, Dog
Mol.Wt.: 29kDa; 29kD(Calculated).
Uniprot: P50402
RRID: AB_2838232

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(88%), Horse(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Emerin Antibody detects endogenous levels of total Emerin.
RRID:
AB_2838232
Cite Format: Affinity Biosciences Cat# DF6266, RRID:AB_2838232.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EDMD; Emd; EMD_HUMAN; Emerin; Emery Dreifuss muscular dystrophy; STA;

Immunogens

Immunogen:
Uniprot:

P50402(EMD_HUMAN) >>Visit HPA database.

Gene(ID):
EMD(2010)
Expression:
P50402 EMD_HUMAN:

Skeletal muscle, heart, colon, testis, ovary and pancreas.

Description:
Emerin is a broadly expressed integral protein of the nuclear inner membrane (1). It contains a LEM domain and binds to several nuclear proteins, such as BAF (barrier-to-autointegration factor) and A- and B-type lamins, which are important in nuclear functions (2-5). Emerin may regulate gene expression through binding to other transcriptional regulators (6,7). Emerin binds to β-catenin and inhibits its nuclear accumulation (8). Recent studies demonstrate that Emerin is required for HIV-1 infectivity (9). Mutations in Emerin are a major cause of Emery-Dreifuss muscular dystrophy (EDMD), a disorder characterized by progressive skeletal muscle weakening (10).
Sequence:
MDNYADLSDTELTTLLRRYNIPHGPVVGSTRRLYEKKIFEYETQRRRLSPPSSSAASSYSFSDLNSTRGDADMYDLPKKEDALLYQSKGYNDDYYEESYFTTRTYGEPESAGPSRAVRQSVTSFPDADAFHHQVHDDDLLSSSEEECKDRERPMYGRDSAYQSITHYRPVSASRSSLDLSYYPTSSSTSFMSSSSSSSSWLTRRAIRPENRAPGAGLGQDRQVPLWGQLLLFLVFVIVLFFIYHFMQAEEGNPF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Rabbit
100
Bovine
88
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P50402 As Substrate

Site PTM Type Enzyme
M1 Acetylation
Y4 Phosphorylation
S8 Phosphorylation
T10 Phosphorylation
Y19 Phosphorylation P00519 (ABL1) , P12931 (SRC)
S29 Phosphorylation
Y34 Phosphorylation
K37 Ubiquitination
Y41 Phosphorylation P00519 (ABL1) , P12931 (SRC)
T43 Phosphorylation
S49 Phosphorylation P17612 (PRKACA)
S52 Phosphorylation
S53 O-Glycosylation
S53 Phosphorylation
S54 O-Glycosylation
S54 Phosphorylation
S57 Phosphorylation
S58 Phosphorylation
Y59 Phosphorylation P12931 (SRC) , P00519 (ABL1)
S60 Phosphorylation
S62 Phosphorylation
S66 Phosphorylation
T67 Phosphorylation
Y74 Phosphorylation P00519 (ABL1) , P12931 (SRC)
K78 Ubiquitination
K79 Ubiquitination
Y85 Phosphorylation P00519 (ABL1) , P12931 (SRC)
S87 O-Glycosylation
S87 Phosphorylation
K88 Ubiquitination
Y90 Phosphorylation P00519 (ABL1) , P12931 (SRC)
Y94 Phosphorylation P12931 (SRC) , P00519 (ABL1)
Y95 Phosphorylation P12931 (SRC) , P00519 (ABL1)
S98 Phosphorylation
Y99 Phosphorylation P12931 (SRC) , P00519 (ABL1)
T101 Phosphorylation
R103 Methylation
T104 Phosphorylation
Y105 Phosphorylation P12931 (SRC) , P00519 (ABL1)
S110 Phosphorylation
S120 Phosphorylation
T122 Phosphorylation
S123 Phosphorylation
S141 Phosphorylation
S142 Phosphorylation
S143 Phosphorylation
Y155 Phosphorylation P00519 (ABL1)
S159 Phosphorylation
Y161 Phosphorylation P00519 (ABL1) , P12931 (SRC)
S163 Phosphorylation
T165 Phosphorylation
Y167 Phosphorylation P00519 (ABL1) , P12931 (SRC)
S171 O-Glycosylation
S171 Phosphorylation
S173 O-Glycosylation
S173 Phosphorylation
S175 Phosphorylation
S176 Phosphorylation
S180 Phosphorylation
Y181 Phosphorylation P12931 (SRC)
Y182 Phosphorylation P12931 (SRC)
S189 Phosphorylation

Research Backgrounds

Function:

Stabilizes and promotes the formation of a nuclear actin cortical network. Stimulates actin polymerization in vitro by binding and stabilizing the pointed end of growing filaments. Inhibits beta-catenin activity by preventing its accumulation in the nucleus. Acts by influencing the nuclear accumulation of beta-catenin through a CRM1-dependent export pathway. Links centrosomes to the nuclear envelope via a microtubule association. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD. Required for proper localization of non-farnesylated prelamin-A/C.

PTMs:

Found in four different phosphorylated forms, three of which appear to be associated with the cell cycle.

Subcellular Location:

Nucleus inner membrane>Single-pass membrane protein>Nucleoplasmic side. Nucleus outer membrane.
Note: Colocalized with BANF1 at the central region of the assembling nuclear rim, near spindle-attachment sites. The accumulation of different intermediates of prelamin-A/C (non-farnesylated or carboxymethylated farnesylated prelamin-A/C) in fibroblasts modify its localization in the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Skeletal muscle, heart, colon, testis, ovary and pancreas.

Subunit Structure:

Interacts with lamins A and C, BANF1, GMCL, BCLAF1 and YTHDC1/YT521. Interacts with TMEM43; the interaction retains emerin in the nuclear inner membrane. Interacts with SUN1 and SUN2 (By similarity). Interacts with ACTB, SPTAN1, F-actin, CTNNB1 and beta-tubulin. Interacts with TMEM201.

Research Fields

· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).

· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).

· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).

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