CRH Antibody | Affinity Biosciences

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FIGURE 2 |Corticotropin-releasing factor (CRF) inhibition of NOD-like receptor pyrin domain-containing protein 6 (NLRP6) expression and Clodistrium butyricum (C.

Product:	CRH Antibody
Catalog:	DF6258
Description:	Rabbit polyclonal antibody to CRH
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Sheep, Dog
Mol.Wt.:	21kDa; 21kD(Calculated).
Uniprot:	P06850
RRID:	AB_2838224

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(92%), Sheep(92%), Dog(92%)

Clonality:

Polyclonal

Specificity:

CRH Antibody detects endogenous levels of total CRH.

RRID:

AB_2838224
Cite Format: Affinity Biosciences Cat# DF6258, RRID:AB_2838224.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Corticoliberin; Corticotropin releasing factor; Corticotropin releasing hormone; CRF; Crh; CRH1;

Immunogens

Immunogen:

Uniprot:

P06850(CRF_HUMAN) >>Visit HPA database.

Gene(ID):

CRH(1392)

Expression:

P06850 CRF_HUMAN:

Produced by the hypothalamus and placenta.

Description:

Corticotropin-releasing hormone is secreted by the paraventricular nucleus (PVN) of the hypothalamus in response to stress. Marked reduction in this protein has been observed in association with Alzheimer disease and autosomal recessive hypothalamic corticotropin deficiency has multiple and potentially fatal metabolic consequences including hypoglycemia and hepatitis. In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. In the placenta it is a marker that determines the length of gestation and the timing of parturition and delivery. A rapid increase in circulating levels of the hormone occurs at the onset of parturition, suggesting that, in addition to its metabolic functions, this protein may act as a trigger for parturition. [provided by RefSeq, Apr 2010]

Sequence:

P06850 CRF_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MRLPLLVSAGVLLVALLPCPPCRALLSRGPVPGARQAPQHPQPLDFFQPPPQSEQPQQPQARPVLLRMGEEYFLRLGNLNKSPAAPLSPASSLLAGGSGSRPSPEQATANFFRVLLQQLLLPRRSLDSPAALAERGARNALGGHQEAPERERRSEEPPISLDLTFHLLREVLEMARAEQLAQQAHSNRKLMEIIGK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

Sheep

Dog

Horse

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P06850 As Substrate

P06850

Site	PTM Type	Enzyme	Source
S125	Phosphorylation		Uniprot

Research Backgrounds

P06850_CRF_HUMAN

Function:

Hormone regulating the release of corticotropin from pituitary gland (By similarity). Induces NLRP6 in intestinal epithelial cells, hence may influence gut microbiota profile (By similarity).

Subcellular Location:

Secreted.

Tissue Specificity:

Produced by the hypothalamus and placenta.

Subunit Structure:

Interacts (via C-terminus) with CRFR1 (via N-terminal extracellular domain).

Family&Domains:

Belongs to the sauvagine/corticotropin-releasing factor/urotensin I family.

Research Fields

· Human Diseases > Substance dependence > Alcoholism.

· Organismal Systems > Nervous system > Long-term depression.

References

1). Corticotropin-releasing factor induces inflammatory cytokines via the NLRP6-inflammatory cytokine axis in a murine model of irritable bowel syndrome. Journal of Digestive Diseases, 2019 (PubMed: 30663229) [IF=3.5]

Application: WB Species: mouse Sample: colonic

FIGURE 2 |Corticotropin-releasing factor (CRF) inhibition of NOD-like receptor pyrin domain-containing protein 6 (NLRP6) expression and Clodistrium butyricum (C. butyricum) restoration of CRF expression in (A-D) the control, irritable bowel syndrome (IBS) model and C. butyricumtreated groups (n = 6 per group) or (E,F) Caco-2 cells. (A) Western blot analysis and (B) gray scale values measured by Quantity One software of CRF expression in colonic tissues.

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CRH Antibody - #DF6258