Prealbumin Antibody | Affinity Biosciences

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(B) Western blotting indicated that expression of TTR gradually increased along with enhanced metastatic potential 23 in different prostate cancer cell lines (HepG2 cells were used as a positive control).

Product:	Prealbumin Antibody
Catalog:	DF6256
Description:	Rabbit polyclonal antibody to Prealbumin
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	16kDa; 16kD(Calculated).
Uniprot:	P02766
RRID:	AB_2838222

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(86%), Dog(100%), Chicken(86%)

Clonality:

Polyclonal

Specificity:

Prealbumin Antibody detects endogenous levels of total Prealbumin.

RRID:

AB_2838222
Cite Format: Affinity Biosciences Cat# DF6256, RRID:AB_2838222.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Amyloid polyneuropathy; Amyloidosis I; ATTR; Carpal tunnel syndrome 1; CTS; CTS1; Dysprealbuminemic euthyroidal hyperthyroxinemia; Dystransthyretinemic hyperthyroxinemia; Epididymis luminal protein 111; HEL111; HsT2651; PALB; Prealbumin amyloidosis type I; Prealbumin; Prealbumin Thyroxine-binding; Senile systemic amyloidosis; TBPA; Thyroxine binding prealbumin; Transthyretin; TTHY_HUMAN; TTR; TTR protein;

Immunogens

Immunogen:

Uniprot:

P02766(TTHY_HUMAN) >>Visit HPA database.

Gene(ID):

TTR(7276)

Expression:

P02766 TTHY_HUMAN:

Detected in serum and cerebrospinal fluid (at protein level). Highly expressed in choroid plexus epithelial cells. Detected in retina pigment epithelium and liver.

Description:

This gene encodes transthyretin, one of the three prealbumins including alpha-1-antitrypsin, transthyretin and orosomucoid. Transthyretin is a carrier protein; it transports thyroid hormones in the plasma and cerebrospinal fluid, and also transports retinol (vitamin A) in the plasma. The protein consists of a tetramer of identical subunits. More than 80 different mutations in this gene have been reported; most mutations are related to amyloid deposition, affecting predominantly peripheral nerve and/or the heart, and a small portion of the gene mutations is non-amyloidogenic. The diseases caused by mutations include amyloidotic polyneuropathy, euthyroid hyperthyroxinaemia, amyloidotic vitreous opacities, cardiomyopathy, oculoleptomeningeal amyloidosis, meningocerebrovascular amyloidosis, carpal tunnel syndrome, etc. [provided by RefSeq, Jan 2009]

Sequence:

P02766 TTHY_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MASHRLLLLCLAGLVFVSEAGPTGTGESKCPLMVKVLDAVRGSPAINVAVHVFRKAADDTWEPFASGKTSESGELHGLTTEEEFVEGIYKVEIDTKSYWKALGISPFHEHAEVVFTANDSGPRRYTIAALLSPYSYSTTAVVTNPKE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Chicken

Rabbit

Zebrafish

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P02766 As Substrate

P02766

Site	PTM Type	Source
C10	S-Nitrosylation	Uniprot
T23	Phosphorylation	Uniprot
T25	Phosphorylation	Uniprot
S72	Phosphorylation	Uniprot
N118	N-Glycosylation	Uniprot
Y125	Phosphorylation	Uniprot
S137	Phosphorylation	Uniprot
T138	Phosphorylation	Uniprot

Research Backgrounds

P02766_TTHY_HUMAN

Function:

Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.

PTMs:

Not glycosylated under normal conditions. Following unfolding, caused for example by variant AMYL-TTR 'Gly-38', the cryptic Asn-118 site is exposed and glycosylated by STT3B-containing OST complex, leading to its degradation by the ER-associated degradation (ERAD) pathway.

Subcellular Location:

Secreted. Cytoplasm.

Tissue Specificity:

Detected in serum and cerebrospinal fluid (at protein level). Highly expressed in choroid plexus epithelial cells. Detected in retina pigment epithelium and liver.

Subunit Structure:

Homotetramer. Dimer of dimers. In the homotetramer, subunits assemble around a central channel that can accommodate two ligand molecules. Interacts with RBP4.

Family&Domains:

Each monomer has two 4-stranded beta sheets and the shape of a prolate ellipsoid. Antiparallel beta-sheet interactions link monomers into dimers. A short loop from each monomer forms the main dimer-dimer interaction. These two pairs of loops separate the opposed, convex beta-sheets of the dimers to form an internal channel.

Belongs to the transthyretin family.

Research Fields

· Organismal Systems > Endocrine system > Thyroid hormone synthesis.

References

1). Tetraiodothyroacetic acid and transthyretin silencing inhibit pro-metastatic effect of L-thyroxin in anoikis-resistant prostate cancer cells through regulation of MAPK/ERK pathway. EXPERIMENTAL CELL RESEARCH (PubMed: 27569004) [IF=3.7]

Application: WB Species: human Sample: HepG2

(B) Western blotting indicated that expression of TTR gradually increased along with enhanced metastatic potential 23 in different prostate cancer cell lines (HepG2 cells were used as a positive control). (C) Western blotting indicated that together with TTR, expressions of XIAP, MMP-2 and VEGF were remarkably higher in anoikis-resistant PC-3 cells in contrast to that of parental cells

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Prealbumin Antibody - #DF6256