GPX1 Antibody - #DF6249

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Product: GPX1 Antibody
Catalog: DF6249
Description: Rabbit polyclonal antibody to GPX1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Rabbit, Dog, Chicken
Mol.Wt.: 22kDa; 22kD(Calculated).
Uniprot: P07203
RRID: AB_2838215

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(91%), Bovine(82%), Horse(100%), Rabbit(90%), Dog(90%), Chicken(80%)
Clonality:
Polyclonal
Specificity:
GPX1 Antibody detects endogenous levels of total GPX1.
RRID:
AB_2838215
Cite Format: Affinity Biosciences Cat# DF6249, RRID:AB_2838215.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AL033363; Cellular glutathione peroxidase; Glutathione peroxidase 1; Glutathione peroxidase; GPx 1; GPx-1; GPX1; GPX1_HUMAN; GPXD; GSHPx-1; GSHPX1; MGC14399; MGC88245;

Immunogens

Immunogen:

A synthesized peptide derived from human GPX1, corresponding to a region within the internal amino acids.

Uniprot:

P07203(GPX1_HUMAN) >>Visit HPA database.

Gene(ID):
GPX1(2876)
Description:
Glutathione peroxidase 1 (GPX1) is a cytosolic selenoprotein which reduces hydrogen peroxide to water (1). GPX1 is the most abundant and ubiquitous among the five GPX isoforms identified so far (2). It is an important component in the anti-oxidative defense in cells and is associated with a variety of disease conditions, such as colon cancer (3), coronary artery disease (4) and insulin resistance (1).
Sequence:
MCAARLAAAAAAAQSVYAFSARPLAGGEPVSLGSLRGKVLLIENVASLUGTTVRDYTQMNELQRRLGPRGLVVLGFPCNQFGHQENAKNEEILNSLKYVRPGGGFEPNFMLFEKCEVNGAGAHPLFAFLREALPAPSDDATALMTDPKLITWSPVCRNDVAWNFEKFLVGPDGVPLRRYSRRFQTIDIEPDIEALLSQGPSCA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Pig
91
Dog
90
Rabbit
90
Bovine
82
Chicken
80
Xenopus
64
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P07203 As Substrate

Site PTM Type Enzyme
S34 Phosphorylation
K88 Acetylation
K97 Ubiquitination
Y98 Phosphorylation P42684 (ABL2) , P00519 (ABL1)
T145 Phosphorylation
K148 Acetylation
S153 Phosphorylation
K166 Ubiquitination
S180 Phosphorylation
S201 Phosphorylation
C202 S-Nitrosylation

Research Backgrounds

Function:

Protects the hemoglobin in erythrocytes from oxidative breakdown.

PTMs:

During periods of oxidative stress, Sec-49 may react with a superoxide radical, irreversibly lose hydroselenide and be converted to dehydroalanine.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homotetramer. Interacts with MIEN1.

Family&Domains:

Belongs to the glutathione peroxidase family.

Research Fields

· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Metabolism > Metabolism of other amino acids > Glutathione metabolism.

· Metabolism > Lipid metabolism > Arachidonic acid metabolism.

· Organismal Systems > Endocrine system > Thyroid hormone synthesis.

References

1). An optimal combination of five main monomer components in Wuzi Yanzong Pill that prevents neural tube defects and reduces apoptosis and oxidative stress. Journal of Ethnopharmacology [IF=5.4]
2). Silymarin protects against acrylamide-induced neurotoxicity via Nrf2 signalling in PC12 cells. FOOD AND CHEMICAL TOXICOLOGY (PubMed: 28137608) [IF=4.3]

Application: WB    Species: rat    Sample: PC12 cells

3). Antioxidative effect of Lactobacillus plantarum Y44 on 2,2\'-azobis(2-methylpropionamidine) dihydrochloride (ABAP)-damaged Caco-2 cells. JOURNAL OF DAIRY SCIENCE (PubMed: 31178173) [IF=3.5]
4). N-acetylcysteine maintains penile length and erectile function in bilateral cavernous nerve crush rat model by reducing penile fibrosis. Asian Journal of Andrology (PubMed: 32394901) [IF=2.9]

Application: WB    Species: Rat    Sample:

Figure 4 Oxidative markers for corpus cavernosum. (a) Result of LOX expression of Western blot. (b) LOX enzyme activity. (c) Representative result of GPX1 expression. Protein expression in a and c was determined by densitometry relative to GAPDH. (d) Measurement of GSH. (e) Statistical analysis of MDA. Compared with the BCNC group, the NAC therapy group significantly decreased the expression and activity of LOX (P <0.05). GPX1 had an upward trend after NAC treatment, but there was no statistical difference (P = 0.0902). The over-expression of MDA was significantly reduced (P <0.05), while the expression of GSH was increased (P <0.05). *P <0.05. BCNC: bilateral cavernous nerve crush; GAPDH: glyceraldehyde phosphate dehydrogenase; GPX1: glutathione peroxidase 1; GSH: glutathione; LOX: lysine oxidase; MDA: malonaldehyde; NAC: N-acetylcysteine; RFUs: relative fluorescent units.
5). Short Photoperiod Reduces Oxidative Stress by Up-Regulating the Nrf2–Keap1 Signaling Pathway in Hamster Kidneys. Journal of Evolutionary Biochemistry and Physiology [IF=0.6]

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