ATP2A2 Antibody | Affinity Biosciences

IF/ICC
Cites

1/5

DF6240 staining A549 cells by IF/ICC.

Fig.

Figure 1 miR-25-3p targets ATP2A2 and CACNA1H, and the expression of miR-25-3p was reduced in the infection-related PTL placenta.

Fig.

Product:	ATP2A2 Antibody
Catalog:	DF6240
Description:	Rabbit polyclonal antibody to ATP2A2
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.:	115kDa; 115kD(Calculated).
Uniprot:	P16615
RRID:	AB_2838206

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(91%), Bovine(82%), Horse(91%), Sheep(82%), Rabbit(100%), Dog(91%), Xenopus(82%)

Clonality:

Polyclonal

Specificity:

ATP2A2 Antibody detects endogenous levels of total ATP2A2.

RRID:

AB_2838206
Cite Format: Affinity Biosciences Cat# DF6240, RRID:AB_2838206.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AT2A2_HUMAN; Atp2a2; ATP2B; ATPase Ca++ transporting cardiac muscle slow twitch 2; Calcium pump 2; Calcium-transporting ATPase sarcoplasmic reticulum type; Calcium-transporting ATPase sarcoplasmic reticulum type slow twitch skeletal muscle isoform; Cardiac Ca2+ ATPase; DAR; DD; Endoplasmic reticulum class 1/2 Ca(2+) ATPase; MGC45367; Sarcoplasmic/endoplasmic reticulum calcium ATPase 2; SERCA 2; SERCA2; serca2a; slow twitch skeletal muscle isoform; SR Ca(2+)-ATPase 2;

Immunogens

Immunogen:

Uniprot:

P16615(AT2A2_HUMAN) >>Visit HPA database.

Gene(ID):

ATP2A2(488)

Expression:

P16615 AT2A2_HUMAN:

Isoform 1 is widely expressed in smooth muscle and nonmuscle tissues such as in adult skin epidermis, with highest expression in liver, pancreas and lung, and intermediate expression in brain, kidney and placenta. Also expressed at lower levels in heart and skeletal muscle. Isoforms 2 and 3 are highly expressed in the heart and slow twitch skeletal muscle. Expression of isoform 3 is predominantly restricted to cardiomyocytes and in close proximity to the sarcolemma. Both isoforms are mildly expressed in lung, kidney, liver, pancreas and placenta. Expression of isoform 3 is amplified during monocytic differentiation and also observed in the fetal heart.

Description:

The ATP2A2 (SERCA2) calcium pump is one of several sarcoplasmic and endoplasmic reticulum Ca2+-ATPases responsible for regulating calcium transport across intracellular membranes (1). Multiple isoforms have been isolated, with ATP2A2a (SERCA2a) found predominantly in the sarcoplasmic reticulum of muscle cells and ATP2A2b (SERCA2b) more ubiquitously expressed in the endoplasmic reticulum of most cell types (2). An isoform containing a truncated carboxy region (ATP2A2c) is expressed in epithelial and hematopoietic cell lines and may be involved in monocyte differentiation (3). Post-translational modification of ATP2A2 (SERCA2), including phosphorylation and tyrosine nitration, modify Ca2+ -ATPase activity and calcium transport (4,5). Mutation in the corresponding ATP2A2 (SERCA2) gene results in Darier disease, a skin disorder characterized by the presence of dark, keratotic papules or rash found on the head and torso (6).

Sequence:

P16615 AT2A2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MENAHTKTVEEVLGHFGVNESTGLSLEQVKKLKERWGSNELPAEEGKTLLELVIEQFEDLLVRILLLAACISFVLAWFEEGEETITAFVEPFVILLILVANAIVGVWQERNAENAIEALKEYEPEMGKVYRQDRKSVQRIKAKDIVPGDIVEIAVGDKVPADIRLTSIKSTTLRVDQSILTGESVSVIKHTDPVPDPRAVNQDKKNMLFSGTNIAAGKAMGVVVATGVNTEIGKIRDEMVATEQERTPLQQKLDEFGEQLSKVISLICIAVWIINIGHFNDPVHGGSWIRGAIYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQMSVCRMFILDRVEGDTCSLNEFTITGSTYAPIGEVHKDDKPVNCHQYDGLVELATICALCNDSALDYNEAKGVYEKVGEATETALTCLVEKMNVFDTELKGLSKIERANACNSVIKQLMKKEFTLEFSRDRKSMSVYCTPNKPSRTSMSKMFVKGAPEGVIDRCTHIRVGSTKVPMTSGVKQKIMSVIREWGSGSDTLRCLALATHDNPLRREEMHLEDSANFIKYETNLTFVGCVGMLDPPRIEVASSVKLCRQAGIRVIMITGDNKGTAVAICRRIGIFGQDEDVTSKAFTGREFDELNPSAQRDACLNARCFARVEPSHKSKIVEFLQSFDEITAMTGDGVNDAPALKKAEIGIAMGSGTAVAKTASEMVLADDNFSTIVAAVEEGRAIYNNMKQFIRYLISSNVGEVVCIFLTAALGFPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMNKPPRNPKEPLISGWLFFRYLAIGCYVGAATVGAAAWWFIAADGGPRVSFYQLSHFLQCKEDNPDFEGVDCAIFESPYPMTMALSVLVTIEMCNALNSLSENQSLLRMPPWENIWLVGSICLSMSLHFLILYVEPLPLIFQITPLNVTQWLMVLKISLPVILMDETLKFVARNYLEPGKECVQPATKSCSFSACTDGISWPFVLLIMPLVIWVYSTDTNFSDMFWS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Pig

Horse

Dog

Bovine

Sheep

Xenopus

Chicken

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P16615 As Substrate

P16615

Site	PTM Type	Enzyme	Source
K30	Ubiquitination		Uniprot
K31	Acetylation		Uniprot
K31	Ubiquitination		Uniprot
K33	Acetylation		Uniprot
S38	Phosphorylation	Q9UQM7 (CAMK2A)	Uniprot
K120	Ubiquitination		Uniprot
K128	Ubiquitination		Uniprot
K143	Ubiquitination		Uniprot
K158	Ubiquitination		Uniprot
R164	Methylation		Uniprot
S167	Phosphorylation		Uniprot
K169	Ubiquitination		Uniprot
S184	Phosphorylation		Uniprot
S186	Phosphorylation		Uniprot
K189	Ubiquitination		Uniprot
T191	Phosphorylation		Uniprot
K205	Ubiquitination		Uniprot
K218	Acetylation		Uniprot
T226	Phosphorylation		Uniprot
T230	Phosphorylation		Uniprot
T242	Phosphorylation		Uniprot
K252	Ubiquitination		Uniprot
S261	Phosphorylation		Uniprot
Y294	Phosphorylation		Uniprot
K328	Ubiquitination		Uniprot
K329	Ubiquitination		Uniprot
S338	Phosphorylation		Uniprot
T345	Phosphorylation		Uniprot
S346	Phosphorylation		Uniprot
K352	Ubiquitination		Uniprot
C364	S-Nitrosylation		Uniprot
K400	Ubiquitination		Uniprot
Y427	Phosphorylation		Uniprot
K431	Ubiquitination		Uniprot
K436	Ubiquitination		Uniprot
T441	Phosphorylation		Uniprot
K451	Ubiquitination		Uniprot
K460	Ubiquitination		Uniprot
K464	Acetylation		Uniprot
K464	Ubiquitination		Uniprot
K476	Acetylation		Uniprot
K476	Ubiquitination		Uniprot
K480	Sumoylation		Uniprot
K481	Acetylation		Uniprot
K481	Ubiquitination		Uniprot
T484	Phosphorylation		Uniprot
S488	Phosphorylation		Uniprot
K492	Ubiquitination		Uniprot
S495	Phosphorylation		Uniprot
Y497	Phosphorylation		Uniprot
C498	S-Nitrosylation		Uniprot
T499	Phosphorylation		Uniprot
K502	Ubiquitination		Uniprot
S504	Phosphorylation		Uniprot
K510	Ubiquitination		Uniprot
K514	Acetylation		Uniprot
K514	Ubiquitination		Uniprot
R523	Methylation		Uniprot
S531	Phosphorylation		Uniprot
T532	Phosphorylation		Uniprot
K533	Ubiquitination		Uniprot
S538	Phosphorylation		Uniprot
K541	Ubiquitination		Uniprot
K543	Ubiquitination		Uniprot
S546	Phosphorylation		Uniprot
R549	Methylation		Uniprot
S553	Phosphorylation		Uniprot
S555	Phosphorylation		Uniprot
T557	Phosphorylation		Uniprot
C560	S-Nitrosylation		Uniprot
S580	Phosphorylation		Uniprot
K585	Sumoylation		Uniprot
S608	Phosphorylation		Uniprot
K611	Ubiquitination		Uniprot
T624	Phosphorylation		Uniprot
K628	Ubiquitination		Uniprot
T648	Phosphorylation		Uniprot
S649	Phosphorylation		Uniprot
K650	Ubiquitination		Uniprot
S663	Phosphorylation		Uniprot
K683	Ubiquitination		Uniprot
S692	Phosphorylation		Uniprot
K711	Ubiquitination		Uniprot
K712	Ubiquitination		Uniprot
S721	Phosphorylation		Uniprot
T723	Phosphorylation		Uniprot
S730	Phosphorylation		Uniprot
S740	Phosphorylation		Uniprot
T741	Phosphorylation		Uniprot
K757	Acetylation		Uniprot
K757	Ubiquitination		Uniprot
S829	Phosphorylation		Uniprot
Y836	Phosphorylation		Uniprot
Y867	Phosphorylation		Uniprot
S973	Phosphorylation		Uniprot
T982	Phosphorylation		Uniprot
Y990	Phosphorylation		Uniprot
K995	Ubiquitination		Uniprot

Research Backgrounds

P16615_AT2A2_HUMAN

Function:

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Isoform 2 is involved in the regulation of the contraction/relaxation cycle. Acts as a regulator of TNFSF11-mediated Ca(2+) signaling pathways via its interaction with TMEM64 which is critical for the TNFSF11-induced CREB1 activation and mitochondrial ROS generation necessary for proper osteoclast generation. Association between TMEM64 and SERCA2 in the ER leads to cytosolic Ca (2+) spiking for activation of NFATC1 and production of mitochondrial ROS, thereby triggering Ca (2+) signaling cascades that promote osteoclast differentiation and activation (By similarity).

PTMs:

Nitrated under oxidative stress. Nitration on the two tyrosine residues inhibits catalytic activity.

Subcellular Location:

Endoplasmic reticulum membrane>Multi-pass membrane protein. Sarcoplasmic reticulum membrane>Multi-pass membrane protein.

Tissue Specificity:

Subunit Structure:

Interacts with sarcolipin (SLN) (By similarity). Interacts with phospholamban (PLN) (By similarity). Interacts with myoregulin (MRLN) (By similarity). Interacts with DWORF (By similarity). Isoform 1 interacts with TRAM2 (via C-terminus). Interacts with HAX1. Interacts with S100A8 and S100A9 (By similarity). Interacts with SLC35G1 and STIM1. Interacts with TMEM203. Interacts with TMEM64 and PDIA3 (By similarity).

Family&Domains:

Ca(2+) and ATP binding cause major rearrangements of the cytoplasmic and transmembrane domains. According to the E1-E2 model, Ca(2+) binding to the cytosolic domain of the pump in the high-affinity E1 conformation is followed by the ATP-dependent phosphorylation of the active site Asp, giving rise to E1P. A conformational change of the phosphoenzyme gives rise to the low-affinity E2P state that exposes the Ca(2+) ions to the lumenal side and promotes Ca(2+) release. Dephosphorylation of the active site Asp mediates the subsequent return to the E1 conformation.

PLN and SLN both have a single transmembrane helix; both occupy a similar binding site that is situated between the ATP2A2 transmembrane helices.

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > Calcium signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).

· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).

· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).

· Organismal Systems > Circulatory system > Cardiac muscle contraction. (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes. (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway. (View pathway)

· Organismal Systems > Digestive system > Pancreatic secretion.

References

1). Osteoporotic bone recovery by a bamboo-structured bioceramic with controlled release of hydroxyapatite nanoparticles. Bioactive Materials, 2022 (PubMed: 35386445) [IF=18.9]

Application: WB Species: Rat Sample:

Fig. 3 Effect of nwHA bioceramics on cocultured osteoporotic osteoblasts. (A) First line: CLSM observations of live (green)/dead (red) staining of osteoporotic osteoblasts cocultured with different nwHA (first line); Second line: CLSM observations of the osteoporotic osteoblasts with F-actin stained with Phalloidin-TRITC (red) and nuclei stained with DAPI (blue). (B) Cell viability of osteoporotic osteoblasts cocultured with different nwHA bioceramics at days 1, 3, and 5. (C) Cell area quantification of osteoporotic osteoblasts from different groups on day 5. (D) qRT-PCR analysis for ATP2A2 and FGF23 gene expressions of osteoporotic osteoblasts from different groups on day 5. (E) Western blotting analysis for ATP2A2 and FGF23 protein expressions of osteoporotic osteoblasts from different groups on day 5; All data are reported as mean ± standard error. ANOVA with Tukey's post hoc test

2). Facilitated Ca2+ homeostasis and attenuated myocardial autophagy contribute to alleviation of diabetic cardiomyopathy after bariatric surgery. AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 2018 (PubMed: 30141985) [IF=4.8]

Application: IHC Species: rat Sample: myocardial Ca2

Fig. 4 |. Impact of bariatric surgery on myocardial Ca2 homeostasis. A: representative Ca2 transients of cardiomyocytes isolated from hearts of the sham, sleeve gastrectomy (SG), and duodenal-jejunal bypass (DJB) groups. B: averaged data of diastolic and peak systolic intracellular Ca2. C: time to 50% decay and time constant of decay of Ca2 transients. D: representative immunohistochemical images of ryanodine receptor 2 (RyR2), sarco(endo)plasmic reticulum Ca2-2ATPase (SERCA2a), and Na/Ca2 exchanger 1 (NCX1) (scale bars 100 m). Brown staining was considered positive.

Application: WB Species: rat Sample: myocardial Ca2

3). A-kinase Anchoring Protein 5 Anchors Protein Kinase A to Mediate PLN/SERCA to Reduce Cardiomyocyte Apoptosis Induced by Hypoxia and Reoxygenation. Biochemistry and Cell Biology, 2022 (PubMed: 35041539) [IF=2.9]

4). Trophoblasts Modulate the Ca2+ Oscillation and Contraction of Myometrial Smooth Muscle Cells by Small Extracellular Vesicle- (sEV-) Mediated Exporting of miR-25-3p during Premature Labor. Oxidative Medicine and Cellular Longevity, 2021 (PubMed: 34413928)

Application: IHC Species: Human Sample: clinical myometrium tissues

Figure 1 miR-25-3p targets ATP2A2 and CACNA1H, and the expression of miR-25-3p was reduced in the infection-related PTL placenta. The binding sites of miR-25-3p on ATP2A2 (the gene name of SERCA2a) and CACNA1H (the gene name of Cav3.2) (a). The binding activity of miR-25-3p on CACNA1H was measured by the dual-luciferase assay (b). The expression of miR-25-3p in the clinical placenta or myometrium tissue was determined by real-time PCR (c, d). The expression of CACNA1H and ATP2A2 in clinical myometrium tissue was detected by real-time PCR (e). Immunohistochemistry detection of Cav3.2 and SERCA2a in the clinical myometrium tissues (f). PTL: preterm labor; FNL: full-term-not-in-labor.

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ATP2A2 Antibody - #DF6240