Product: ATP2A2 Antibody
Catalog: DF6240
Description: Rabbit polyclonal antibody to ATP2A2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 115kDa; 115kD(Calculated).
Uniprot: P16615
RRID: AB_2838206

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(91%), Bovine(82%), Horse(91%), Sheep(82%), Rabbit(100%), Dog(91%), Xenopus(82%)
Clonality:
Polyclonal
Specificity:
ATP2A2 Antibody detects endogenous levels of total ATP2A2.
RRID:
AB_2838206
Cite Format: Affinity Biosciences Cat# DF6240, RRID:AB_2838206.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AT2A2_HUMAN; Atp2a2; ATP2B; ATPase Ca++ transporting cardiac muscle slow twitch 2; Calcium pump 2; Calcium-transporting ATPase sarcoplasmic reticulum type; Calcium-transporting ATPase sarcoplasmic reticulum type slow twitch skeletal muscle isoform; Cardiac Ca2+ ATPase; DAR; DD; Endoplasmic reticulum class 1/2 Ca(2+) ATPase; MGC45367; Sarcoplasmic/endoplasmic reticulum calcium ATPase 2; SERCA 2; SERCA2; serca2a; slow twitch skeletal muscle isoform; SR Ca(2+)-ATPase 2;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P16615 AT2A2_HUMAN:

Isoform 1 is widely expressed in smooth muscle and nonmuscle tissues such as in adult skin epidermis, with highest expression in liver, pancreas and lung, and intermediate expression in brain, kidney and placenta. Also expressed at lower levels in heart and skeletal muscle. Isoforms 2 and 3 are highly expressed in the heart and slow twitch skeletal muscle. Expression of isoform 3 is predominantly restricted to cardiomyocytes and in close proximity to the sarcolemma. Both isoforms are mildly expressed in lung, kidney, liver, pancreas and placenta. Expression of isoform 3 is amplified during monocytic differentiation and also observed in the fetal heart.

Description:
The ATP2A2 (SERCA2) calcium pump is one of several sarcoplasmic and endoplasmic reticulum Ca2+-ATPases responsible for regulating calcium transport across intracellular membranes (1). Multiple isoforms have been isolated, with ATP2A2a (SERCA2a) found predominantly in the sarcoplasmic reticulum of muscle cells and ATP2A2b (SERCA2b) more ubiquitously expressed in the endoplasmic reticulum of most cell types (2). An isoform containing a truncated carboxy region (ATP2A2c) is expressed in epithelial and hematopoietic cell lines and may be involved in monocyte differentiation (3). Post-translational modification of ATP2A2 (SERCA2), including phosphorylation and tyrosine nitration, modify Ca2+ -ATPase activity and calcium transport (4,5). Mutation in the corresponding ATP2A2 (SERCA2) gene results in Darier disease, a skin disorder characterized by the presence of dark, keratotic papules or rash found on the head and torso (6).
Sequence:
MENAHTKTVEEVLGHFGVNESTGLSLEQVKKLKERWGSNELPAEEGKTLLELVIEQFEDLLVRILLLAACISFVLAWFEEGEETITAFVEPFVILLILVANAIVGVWQERNAENAIEALKEYEPEMGKVYRQDRKSVQRIKAKDIVPGDIVEIAVGDKVPADIRLTSIKSTTLRVDQSILTGESVSVIKHTDPVPDPRAVNQDKKNMLFSGTNIAAGKAMGVVVATGVNTEIGKIRDEMVATEQERTPLQQKLDEFGEQLSKVISLICIAVWIINIGHFNDPVHGGSWIRGAIYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQMSVCRMFILDRVEGDTCSLNEFTITGSTYAPIGEVHKDDKPVNCHQYDGLVELATICALCNDSALDYNEAKGVYEKVGEATETALTCLVEKMNVFDTELKGLSKIERANACNSVIKQLMKKEFTLEFSRDRKSMSVYCTPNKPSRTSMSKMFVKGAPEGVIDRCTHIRVGSTKVPMTSGVKQKIMSVIREWGSGSDTLRCLALATHDNPLRREEMHLEDSANFIKYETNLTFVGCVGMLDPPRIEVASSVKLCRQAGIRVIMITGDNKGTAVAICRRIGIFGQDEDVTSKAFTGREFDELNPSAQRDACLNARCFARVEPSHKSKIVEFLQSFDEITAMTGDGVNDAPALKKAEIGIAMGSGTAVAKTASEMVLADDNFSTIVAAVEEGRAIYNNMKQFIRYLISSNVGEVVCIFLTAALGFPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMNKPPRNPKEPLISGWLFFRYLAIGCYVGAATVGAAAWWFIAADGGPRVSFYQLSHFLQCKEDNPDFEGVDCAIFESPYPMTMALSVLVTIEMCNALNSLSENQSLLRMPPWENIWLVGSICLSMSLHFLILYVEPLPLIFQITPLNVTQWLMVLKISLPVILMDETLKFVARNYLEPGKECVQPATKSCSFSACTDGISWPFVLLIMPLVIWVYSTDTNFSDMFWS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Pig
91
Horse
91
Dog
91
Bovine
82
Sheep
82
Xenopus
82
Chicken
73
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P16615 As Substrate

Site PTM Type Enzyme
K30 Ubiquitination
K31 Acetylation
K31 Ubiquitination
K33 Acetylation
S38 Phosphorylation Q9UQM7 (CAMK2A)
K120 Ubiquitination
K128 Ubiquitination
K143 Ubiquitination
K158 Ubiquitination
R164 Methylation
S167 Phosphorylation
K169 Ubiquitination
S184 Phosphorylation
S186 Phosphorylation
K189 Ubiquitination
T191 Phosphorylation
K205 Ubiquitination
K218 Acetylation
T226 Phosphorylation
T230 Phosphorylation
T242 Phosphorylation
K252 Ubiquitination
S261 Phosphorylation
Y294 Phosphorylation
K328 Ubiquitination
K329 Ubiquitination
S338 Phosphorylation
T345 Phosphorylation
S346 Phosphorylation
K352 Ubiquitination
C364 S-Nitrosylation
K400 Ubiquitination
Y427 Phosphorylation
K431 Ubiquitination
K436 Ubiquitination
T441 Phosphorylation
K451 Ubiquitination
K460 Ubiquitination
K464 Acetylation
K464 Ubiquitination
K476 Acetylation
K476 Ubiquitination
K480 Sumoylation
K481 Acetylation
K481 Ubiquitination
T484 Phosphorylation
S488 Phosphorylation
K492 Ubiquitination
S495 Phosphorylation
Y497 Phosphorylation
C498 S-Nitrosylation
T499 Phosphorylation
K502 Ubiquitination
S504 Phosphorylation
K510 Ubiquitination
K514 Acetylation
K514 Ubiquitination
R523 Methylation
S531 Phosphorylation
T532 Phosphorylation
K533 Ubiquitination
S538 Phosphorylation
K541 Ubiquitination
K543 Ubiquitination
S546 Phosphorylation
R549 Methylation
S553 Phosphorylation
S555 Phosphorylation
T557 Phosphorylation
C560 S-Nitrosylation
S580 Phosphorylation
K585 Sumoylation
S608 Phosphorylation
K611 Ubiquitination
T624 Phosphorylation
K628 Ubiquitination
T648 Phosphorylation
S649 Phosphorylation
K650 Ubiquitination
S663 Phosphorylation
K683 Ubiquitination
S692 Phosphorylation
K711 Ubiquitination
K712 Ubiquitination
S721 Phosphorylation
T723 Phosphorylation
S730 Phosphorylation
S740 Phosphorylation
T741 Phosphorylation
K757 Acetylation
K757 Ubiquitination
S829 Phosphorylation
Y836 Phosphorylation
Y867 Phosphorylation
S973 Phosphorylation
T982 Phosphorylation
Y990 Phosphorylation
K995 Ubiquitination

Research Backgrounds

Function:

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Isoform 2 is involved in the regulation of the contraction/relaxation cycle. Acts as a regulator of TNFSF11-mediated Ca(2+) signaling pathways via its interaction with TMEM64 which is critical for the TNFSF11-induced CREB1 activation and mitochondrial ROS generation necessary for proper osteoclast generation. Association between TMEM64 and SERCA2 in the ER leads to cytosolic Ca (2+) spiking for activation of NFATC1 and production of mitochondrial ROS, thereby triggering Ca (2+) signaling cascades that promote osteoclast differentiation and activation (By similarity).

PTMs:

Nitrated under oxidative stress. Nitration on the two tyrosine residues inhibits catalytic activity.

Subcellular Location:

Endoplasmic reticulum membrane>Multi-pass membrane protein. Sarcoplasmic reticulum membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform 1 is widely expressed in smooth muscle and nonmuscle tissues such as in adult skin epidermis, with highest expression in liver, pancreas and lung, and intermediate expression in brain, kidney and placenta. Also expressed at lower levels in heart and skeletal muscle. Isoforms 2 and 3 are highly expressed in the heart and slow twitch skeletal muscle. Expression of isoform 3 is predominantly restricted to cardiomyocytes and in close proximity to the sarcolemma. Both isoforms are mildly expressed in lung, kidney, liver, pancreas and placenta. Expression of isoform 3 is amplified during monocytic differentiation and also observed in the fetal heart.

Subunit Structure:

Interacts with sarcolipin (SLN) (By similarity). Interacts with phospholamban (PLN) (By similarity). Interacts with myoregulin (MRLN) (By similarity). Interacts with DWORF (By similarity). Isoform 1 interacts with TRAM2 (via C-terminus). Interacts with HAX1. Interacts with S100A8 and S100A9 (By similarity). Interacts with SLC35G1 and STIM1. Interacts with TMEM203. Interacts with TMEM64 and PDIA3 (By similarity).

Family&Domains:

Ca(2+) and ATP binding cause major rearrangements of the cytoplasmic and transmembrane domains. According to the E1-E2 model, Ca(2+) binding to the cytosolic domain of the pump in the high-affinity E1 conformation is followed by the ATP-dependent phosphorylation of the active site Asp, giving rise to E1P. A conformational change of the phosphoenzyme gives rise to the low-affinity E2P state that exposes the Ca(2+) ions to the lumenal side and promotes Ca(2+) release. Dephosphorylation of the active site Asp mediates the subsequent return to the E1 conformation.

PLN and SLN both have a single transmembrane helix; both occupy a similar binding site that is situated between the ATP2A2 transmembrane helices.

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).

· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).

· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).

· Organismal Systems > Circulatory system > Cardiac muscle contraction.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

· Organismal Systems > Digestive system > Pancreatic secretion.

References

1). Osteoporotic bone recovery by a bamboo-structured bioceramic with controlled release of hydroxyapatite nanoparticles. Bioactive Materials, 2022 (PubMed: 35386445) [IF=18.9]

Application: WB    Species: Rat    Sample:

Fig. 3 Effect of nwHA bioceramics on cocultured osteoporotic osteoblasts. (A) First line: CLSM observations of live (green)/dead (red) staining of osteoporotic osteoblasts cocultured with different nwHA (first line); Second line: CLSM observations of the osteoporotic osteoblasts with F-actin stained with Phalloidin-TRITC (red) and nuclei stained with DAPI (blue). (B) Cell viability of osteoporotic osteoblasts cocultured with different nwHA bioceramics at days 1, 3, and 5. (C) Cell area quantification of osteoporotic osteoblasts from different groups on day 5. (D) qRT-PCR analysis for ATP2A2 and FGF23 gene expressions of osteoporotic osteoblasts from different groups on day 5. (E) Western blotting analysis for ATP2A2 and FGF23 protein expressions of osteoporotic osteoblasts from different groups on day 5; All data are reported as mean ± standard error. ANOVA with Tukey's post hoc test

2). Facilitated Ca2+ homeostasis and attenuated myocardial autophagy contribute to alleviation of diabetic cardiomyopathy after bariatric surgery. AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 2018 (PubMed: 30141985) [IF=4.8]

Application: IHC    Species: rat    Sample: myocardial Ca2

Fig. 4 |. Impact of bariatric surgery on myocardial Ca2 homeostasis. A: representative Ca2 transients of cardiomyocytes isolated from hearts of the sham, sleeve gastrectomy (SG), and duodenal-jejunal bypass (DJB) groups. B: averaged data of diastolic and peak systolic intracellular Ca2. C: time to 50% decay and time constant of decay of Ca2 transients. D: representative immunohistochemical images of ryanodine receptor 2 (RyR2), sarco(endo)plasmic reticulum Ca2-2ATPase (SERCA2a), and Na/Ca2 exchanger 1 (NCX1) (scale bars 100 m). Brown staining was considered positive.

Application: WB    Species: rat    Sample: myocardial Ca2

Fig. 4 |. Impact of bariatric surgery on myocardial Ca2 homeostasis. A: representative Ca2 transients of cardiomyocytes isolated from hearts of the sham, sleeve gastrectomy (SG), and duodenal-jejunal bypass (DJB) groups. B: averaged data of diastolic and peak systolic intracellular Ca2. C: time to 50% decay and time constant of decay of Ca2 transients. D: representative immunohistochemical images of ryanodine receptor 2 (RyR2), sarco(endo)plasmic reticulum Ca2-2ATPase (SERCA2a), and Na/Ca2 exchanger 1 (NCX1) (scale bars 100 m). Brown staining was considered positive.

3). A-kinase Anchoring Protein 5 Anchors Protein Kinase A to Mediate PLN/SERCA to Reduce Cardiomyocyte Apoptosis Induced by Hypoxia and Reoxygenation. Biochemistry and Cell Biology, 2022 (PubMed: 35041539) [IF=2.9]

4). Trophoblasts Modulate the Ca2+ Oscillation and Contraction of Myometrial Smooth Muscle Cells by Small Extracellular Vesicle- (sEV-) Mediated Exporting of miR-25-3p during Premature Labor. Oxidative Medicine and Cellular Longevity, 2021 (PubMed: 34413928)

Application: IHC    Species: Human    Sample: clinical myometrium tissues

Figure 1 miR-25-3p targets ATP2A2 and CACNA1H, and the expression of miR-25-3p was reduced in the infection-related PTL placenta. The binding sites of miR-25-3p on ATP2A2 (the gene name of SERCA2a) and CACNA1H (the gene name of Cav3.2) (a). The binding activity of miR-25-3p on CACNA1H was measured by the dual-luciferase assay (b). The expression of miR-25-3p in the clinical placenta or myometrium tissue was determined by real-time PCR (c, d). The expression of CACNA1H and ATP2A2 in clinical myometrium tissue was detected by real-time PCR (e). Immunohistochemistry detection of Cav3.2 and SERCA2a in the clinical myometrium tissues (f). PTL: preterm labor; FNL: full-term-not-in-labor.

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