TYMP Antibody | Affinity Biosciences

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Western blot analysis of extracts from EC304 cells, using TYMP Antibody.

Product:	TYMP Antibody
Catalog:	DF6237
Description:	Rabbit polyclonal antibody to TYMP
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	50kDa; 50kD(Calculated).
Uniprot:	P19971
RRID:	AB_2838203

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

TYMP Antibody detects endogenous levels of total TYMP.

RRID:

AB_2838203
Cite Format: Affinity Biosciences Cat# DF6237, RRID:AB_2838203.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ECGF 1; ECGF; ECGF1; Endothelial cell growth factor 1; Endothelial cell growth factor 1 platelet derived; Endothelial cell growth factor, platelet-derived; Gliostatin; hPD ECGF; MEDPS1; MNGIE; MTDPS1; PD ECGF; PD-ECGF; PDECGF; PDEGF; Platelet derived endothelial cell growth factor; Platelet derived endothelial growth factor; Platelet-derived endothelial cell growth factor; TdRPase; Thymidine phosphorylase; TP; Tymp; TYPH_HUMAN;

Immunogens

Immunogen:

Uniprot:

P19971(TYPH_HUMAN) >>Visit HPA database.

Gene(ID):

TYMP(1890)

Description:

Thymidine phosphorylase (TP) is a platelet-derived endothelial cell growth factor (PD-ECGF) that catalyzes the formation of thymine and 2-deoxy-D-ribose-1-phosphate from thymidine and orthophosphate (1). This intracellular enzyme is capable of both promoting angiogenesis and inhibiting apoptosis. Thymidine phosphorylase catalytic activity is required for its angiogenic function (2,3). Increased expression of TP/PD-ECGF is seen in a wide variety of different solid tumors and inflammatory diseases and is often associated with poor prognosis (4,5). Alternatively, TP can activate fluorouracil derivative (DFUR) prodrugs and increase the antitumor activity of the related treatment (1,5). The use of thymidine phosphorylase as a cancer therapeutic target has been studied extensively, with emphasis on either inhibiting TP enzymatic activity or increasing enzyme induction with concomitant DFUR treatment (1,5).

Sequence:

P19971 TYPH_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAALMTPGTGAPPAPGDFSGEGSQGLPDPSPEPKQLPELIRMKRDGGRLSEADIRGFVAAVVNGSAQGAQIGAMLMAIRLRGMDLEETSVLTQALAQSGQQLEWPEAWRQQLVDKHSTGGVGDKVSLVLAPALAACGCKVPMISGRGLGHTGGTLDKLESIPGFNVIQSPEQMQVLLDQAGCCIVGQSEQLVPADGILYAARDVTATVDSLPLITASILSKKLVEGLSALVVDVKFGGAAVFPNQEQARELAKTLVGVGASLGLRVAAALTAMDKPLGRCVGHALEVEEALLCMDGAGPPDLRDLVTTLGGALLWLSGHAGTQAQGAARVAAALDDGSALGRFERMLAAQGVDPGLARALCSGSPAERRQLLPRAREQEELLAPADGTVELVRALPLALVLHELGAGRSRAGEPLRLGVGAELLVDVGQRLRRGTPWLRVHRDGPALSGPQSRALQEALVLSDRAPFAAPSPFAELVLPPQQ

PTMs - P19971 As Substrate

P19971

Site	PTM Type	Source
T6	Phosphorylation	Uniprot
S19	Phosphorylation	Uniprot
S23	Phosphorylation	Uniprot
S30	Phosphorylation	Uniprot
S50	Phosphorylation	Uniprot
T154	Phosphorylation	Uniprot
T271	Phosphorylation	Uniprot
K275	Acetylation	Uniprot
S409	Phosphorylation	Uniprot
T435	Phosphorylation	Uniprot
S471	Phosphorylation	Uniprot

Research Backgrounds

P19971_TYPH_HUMAN

Function:

May have a role in maintaining the integrity of the blood vessels. Has growth promoting activity on endothelial cells, angiogenic activity in vivo and chemotactic activity on endothelial cells in vitro.

Catalyzes the reversible phosphorolysis of thymidine. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family.

Research Fields

· Human Diseases > Cancers: Specific types > Bladder cancer. (View pathway)

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

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For Research Use Only.
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TYMP Antibody - #DF6237