MAGED1 Antibody - #DF6235

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Product: MAGED1 Antibody
Catalog: DF6235
Description: Rabbit polyclonal antibody to MAGED1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 86kDa; 86kD(Calculated).
Uniprot: Q9Y5V3
RRID: AB_2838201

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(83%), Bovine(83%), Sheep(83%), Rabbit(100%), Dog(83%)
Clonality:
Polyclonal
Specificity:
MAGED1 Antibody detects endogenous levels of total MAGED1.
RRID:
AB_2838201
Cite Format: Affinity Biosciences Cat# DF6235, RRID:AB_2838201.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DLXIN 1; MAGD1_HUMAN; MAGE D1 antigen; MAGE tumor antigen CCF; MAGE-D1 antigen; Maged1; Melanoma antigen family D 1; Melanoma associated antigen D1; Melanoma-associated antigen D1; Neurotrophin receptor interacting MAGE homolog; Neurotrophin receptor-interacting MAGE homolog; NRAGE; PP2250;

Immunogens

Immunogen:
Uniprot:

Q9Y5V3(MAGD1_HUMAN) >>Visit HPA database.

Gene(ID):
MAGED1(9500)
Expression:
Q9Y5V3 MAGD1_HUMAN:

Expressed in bone marrow stromal cells from both multiple myeloma patients and healthy donors. Seems to be ubiquitously expressed.

Description:
MAGE-D1, also known as NRAGE or Dlxin-1, is a member of the MAGE family of proteins. Identified as a p75 neurotrophin receptor intracellular binding protein, MAGE-D1 induces developmental apoptosis of motoneurons, and is required for p75NTR-dependent apoptosis in sympathetic neurons. It is suggested that MAGE-D1 can suppress the motility and adhesion response of tumor cells. By forming a hetercomplex with Dlx/Msx family homeodomain proteins and Necdin, MAGE-D1 modulates the function of Dlx/Msxhomeodomain during terminal differentiation and maturation of neurons. MAGE-D1 is also involved in the phosphorylation of IKK-alpha/beta, and subsequent transcriptional activation of the p65 subunit of NF-kappaB, via the XIAP-Tak1-Tab1 complex. (20100315, 19639218, 17453828, 15272023) G.
Sequence:
MAQKMDCGAGLLGFQAEASVEDSALLMQTLMEAIQISEAPPTNQATAAASPQSSQPPTANEMADIQVSAAAARPKSAFKVQNATTKGPNGVYDFSQAHNAKDVPNTQPKAAFKSQNATPKGPNAAYDFSQAATTGELAANKSEMAFKAQNATTKVGPNATYNFSQSLNANDLANSRPKTPFKAWNDTTKAPTADTQTQNVNQAKMATSQADIETDPGISEPDGATAQTSADGSQAQNLESRTIIRGKRTRKINNLNVEENSSGDQRRAPLAAGTWRSAPVPVTTQNPPGAPPNVLWQTPLAWQNPSGWQNQTARQTPPARQSPPARQTPPAWQNPVAWQNPVIWPNPVIWQNPVIWPNPIVWPGPVVWPNPLAWQNPPGWQTPPGWQTPPGWQGPPDWQGPPDWPLPPDWPLPPDWPLPTDWPLPPDWIPADWPIPPDWQNLRPSPNLRPSPNSRASQNPGAAQPRDVALLQERANKLVKYLMLKDYTKVPIKRSEMLRDIIREYTDVYPEIIERACFVLEKKFGIQLKEIDKEEHLYILISTPESLAGILGTTKDTPKLGLLLVILGVIFMNGNRASEAVLWEALRKMGLRPGVRHPLLGDLRKLLTYEFVKQKYLDYRRVPNSNPPEYEFLWGLRSYHETSKMKVLRFIAEVQKRDPRDWTAQFMEAADEALDALDAAAAEAEARAEARTRMGIGDEAVSGPWSWDDIEFELLTWDEEGDFGDPWSRIPFTFWARYHQNARSRFPQTFAGPIIGPGGTASANFAANFGAIGFFWVE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Pig
83
Bovine
83
Sheep
83
Dog
83
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y5V3 As Substrate

Site PTM Type Enzyme
S76 Phosphorylation
K79 Ubiquitination
T85 Phosphorylation
K86 Ubiquitination
Y92 Phosphorylation
S95 Phosphorylation
K101 Acetylation
K101 Ubiquitination
K109 Acetylation
K109 Ubiquitination
K113 Ubiquitination
K120 Ubiquitination
Y126 Phosphorylation
S129 Phosphorylation
K141 Ubiquitination
K147 Methylation
K147 Ubiquitination
K154 Ubiquitination
T160 Phosphorylation
Y161 Phosphorylation
S166 Phosphorylation
T179 Phosphorylation
K182 Ubiquitination
K189 Ubiquitination
K247 Ubiquitination
K251 Ubiquitination
T274 Phosphorylation
T312 Phosphorylation
T316 Phosphorylation
S322 Phosphorylation
S457 Phosphorylation
K480 Ubiquitination
Y481 Phosphorylation
K485 Ubiquitination
K489 Ubiquitination
K493 Ubiquitination
Y505 Phosphorylation
K522 Ubiquitination
K523 Ubiquitination
K529 Ubiquitination
K555 Ubiquitination
S578 Phosphorylation
K588 Ubiquitination
R604 Methylation
K605 Ubiquitination
K613 Ubiquitination
K615 Ubiquitination
Y630 Phosphorylation
S638 Phosphorylation
T642 Phosphorylation
S643 Phosphorylation
K644 Ubiquitination
K656 Ubiquitination

Research Backgrounds

Function:

Involved in the apoptotic response after nerve growth factor (NGF) binding in neuronal cells. Inhibits cell cycle progression, and facilitates NGFR-mediated apoptosis. May act as a regulator of the function of DLX family members. May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. Plays a role in the circadian rhythm regulation. May act as RORA co-regulator, modulating the expression of core clock genes such as ARNTL/BMAL1 and NFIL3, induced, or NR1D1, repressed.

Subcellular Location:

Cytoplasm. Cell membrane>Peripheral membrane protein. Nucleus.
Note: Expression shifts from the cytoplasm to the plasma membrane upon stimulation with NGF.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in bone marrow stromal cells from both multiple myeloma patients and healthy donors. Seems to be ubiquitously expressed.

Subunit Structure:

Interacts with DLX5, DLX7 and MSX2 and forms homomultimers. Interacts with UNC5A. Interacts with TRIM28 and PJA1. Interacts with NGFR/p75NTR and RORA.

Research Fields

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

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