AMBRA1 Antibody - #DF6228
(1)
| Product: | AMBRA1 Antibody |
| Catalog: | DF6228 |
| Description: | Rabbit polyclonal antibody to AMBRA1 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| Mol.Wt.: | 133kDa; 143kD(Calculated). |
| Uniprot: | Q9C0C7 |
| RRID: | AB_2838194 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(83%)
Clonality:
Polyclonal
Specificity:
AMBRA1 Antibody detects endogenous levels of total AMBRA1.
RRID:
AB_2838194
Cite Format: Affinity Biosciences Cat# DF6228, RRID:AB_2838194.
Cite Format: Affinity Biosciences Cat# DF6228, RRID:AB_2838194.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
Activating molecule in beclin 1 regulated autophagy; Activating molecule in BECN1 regulated autophagy protein 1; Activating molecule in BECN1-regulated autophagy protein 1; Ambra1; AMRA1_HUMAN; FLJ20294; Hypothetical protein FLJ20294; Hypothetical protein LOC55626; KIAA1736; MGC33725; WDR94;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Description:
WD-repeats are motifs that are found in a variety of proteins and are characterized by a conserved core of 40-60 amino acids that commonly form a tertiary propeller structure. While proteins that contain WD-repeats participate in a wide range of cellular functions, they are generally involved in regulatory mechanisms concerning chromatin assembly, cell cycle control, signal transduction, RNA processing, apoptosis and vesicular trafficking. AMBRA1 (Activating molecule in BECN1-regulated autophagy protein 1), also known as WDR94 or KIAA1736, is a 1,298 amino acid protein that contains three WD repeats. Localized to cytoplasmic vesicles, AMBRA1 functions to control protein turnover, cell proliferation and cell survival during neuronal development, thereby playing an important role in autophagy and the development of the nervous system. Multiple isoforms of AMBRA1 exist due to alternative spicing events.
Sequence:
- Q9C0C7 AMRA1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKVVPEKNAVRILWGRERGARAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQSTVQDSATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTAECCQHLGILCLCSRCSGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHREIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLATESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPQTQQAQEMLNNNIESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWQVPSSFESVPSSGSQLPPLERTEGQTPSSSRLELSSSASPQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGVEYYWDQLNETVFTVHSNSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPATSVTSQGTQTLALQLQNAETQTEREVPEPGTAASGPGEGEGSEYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAAHTSSPQPSTSRGLLPEAGQLAERGLSPRTASWDQPGTPGREPTQPTLPSSSPVPIPVSLPSAEGPTLHCELTNNNHLLDGGSSRGDAAGPRGEPRNR
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
83
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9C0C7 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Phosphorylation | Uniprot | ||
| K2 | Ubiquitination | Uniprot | |
| K7 | Ubiquitination | Uniprot | |
| K36 | Ubiquitination | Uniprot | |
| K41 | Ubiquitination | Uniprot | |
| S52 | Phosphorylation | P42345 (MTOR) | Uniprot |
| K175 | Ubiquitination | Uniprot | |
| R226 | Methylation | Uniprot | |
| S328 | Phosphorylation | Uniprot | |
| S387 | Phosphorylation | Uniprot | |
| T391 | Phosphorylation | Uniprot | |
| R392 | Methylation | Uniprot | |
| S394 | Phosphorylation | Uniprot | |
| S443 | Phosphorylation | Uniprot | |
| S465 | Phosphorylation | Uniprot | |
| S628 | Phosphorylation | Uniprot | |
| S629 | Phosphorylation | Uniprot | |
| S635 | Phosphorylation | Uniprot | |
| S639 | Phosphorylation | Uniprot | |
| S696 | Phosphorylation | Uniprot | |
| S722 | Phosphorylation | Uniprot | |
| R730 | Methylation | Uniprot | |
| R747 | Methylation | Uniprot | |
| R754 | Methylation | Uniprot | |
| R795 | Methylation | Uniprot | |
| S797 | Phosphorylation | Uniprot | |
| S800 | Phosphorylation | Uniprot | |
| R808 | Methylation | Uniprot | |
| Y814 | Phosphorylation | Uniprot | |
| R824 | Methylation | Uniprot | |
| Y1026 | Phosphorylation | Uniprot | |
| T1028 | Phosphorylation | Uniprot | |
| S1043 | Phosphorylation | Uniprot | |
| S1192 | Phosphorylation | Uniprot | |
| T1203 | Phosphorylation | Uniprot | |
| S1204 | Phosphorylation | Uniprot | |
| S1205 | Phosphorylation | Uniprot | |
| T1238 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
Regulates autophagy and development of the nervous system. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation (By similarity).
Subcellular Location:
Cytoplasmic vesicle>Autophagosome.
Note: Localizes also to discrete punctae along the ciliary axoneme.
Subunit Structure:
Interacts with BECN1. Probably forms a complex with BECN1 and PIK3C3 (By similarity). Interacts with BECN2.
Research Fields
· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)
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