HADH Antibody - #DF6221

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Product: HADH Antibody
Catalog: DF6221
Description: Rabbit polyclonal antibody to HADH
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 34kDa; 34kD(Calculated).
Uniprot: Q16836
RRID: AB_2838187

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Zebrafish(100%), Bovine(89%), Horse(100%), Sheep(89%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
HADH Antibody detects endogenous levels of total HADH.
RRID:
AB_2838187
Cite Format: Affinity Biosciences Cat# DF6221, RRID:AB_2838187.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

3 hydroxyacyl Coenzyme A dehydrogenase; HAD; HADH; HADH1; HADHSC; HADHSC, formerly; HADSC, formerly; HCDH; HCDH_HUMAN; HHF4; Hydroxyacyl CoA dehydrogenase; Hydroxyacyl-coenzyme A dehydrogenase; hydroxyacyl-coenzyme A dehydrogenase, mitochondrial; L 3 hydroxyacyl Coenzyme A dehydrogenase short chain; M SCHAD; Medium and short chain L 3 hydroxyacyl coenzyme A dehydrogenase; Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase; MGC8392; mitochondrial; MSCHAD; OTTHUMP00000162626; OTTHUMP00000219688; SCHAD; SCHAD, formerly; Short chain 3 hydroxyacyl CoA dehydrogenase mitochondrial; short chain 3-hydroxyacyl-coa dehydrogenase; Short-chain 3-hydroxyacyl-CoA dehydrogenase;

Immunogens

Immunogen:
Uniprot:

Q16836(HCDH_HUMAN) >>Visit HPA database.

Gene(ID):
HADH(3033)
Expression:
Q16836 HCDH_HUMAN:

Expressed in liver, kidney, pancreas, heart and skeletal muscle.

Description:
This gene is a member of the 3-hydroxyacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene cause one form of familial hyperinsulinemic hypoglycemia. The human genome contains a related pseudogene of this gene on chromosome 15. [provided by RefSeq, May 2010]
Sequence:
MAFVTRQFMRSVSSSSTASASAKKIIVKHVTVIGGGLMGAGIAQVAAATGHTVVLVDQTEDILAKSKKGIEESLRKVAKKKFAENLKAGDEFVEKTLSTIATSTDAASVVHSTDLVVEAIVENLKVKNELFKRLDKFAAEHTIFASNTSSLQITSIANATTRQDRFAGLHFFNPVPVMKLVEVIKTPMTSQKTFESLVDFSKALGKHPVSCKDTPGFIVNRLLVPYLMEAIRLYERGDASKEDIDTAMKLGAGYPMGPFELLDYVGLDTTKFIVDGWHEMDAENPLHQPSPSLNKLVAENKFGKKTGEGFYKYK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Bovine
89
Sheep
89
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q16836 As Substrate

Site PTM Type Enzyme
S13 Phosphorylation
K68 Ubiquitination
S73 Phosphorylation
R75 Methylation
K76 Acetylation
K80 Acetylation
K81 Acetylation
K87 Acetylation
K87 Ubiquitination
K127 Ubiquitination
K132 Acetylation
K179 Acetylation
K185 Acetylation
K185 Ubiquitination
K192 Acetylation
K192 Ubiquitination
T193 Phosphorylation
S196 Phosphorylation
S201 Phosphorylation
K202 Acetylation
K202 Ubiquitination
K206 Ubiquitination
K212 Acetylation
K212 Ubiquitination
T214 Phosphorylation
Y226 Phosphorylation
Y234 Phosphorylation
K241 Acetylation
K241 Ubiquitination
K249 Acetylation
Y264 Phosphorylation
T269 Phosphorylation
T270 Phosphorylation
S290 Phosphorylation
K301 Ubiquitination
K304 Acetylation
K312 Acetylation
K312 Methylation
K312 Ubiquitination
Y313 Phosphorylation

Research Backgrounds

Function:

Plays an essential role in the mitochondrial beta-oxidation of short chain fatty acids. Exerts it highest activity toward 3-hydroxybutyryl-CoA.

PTMs:

Succinylation at Lys-81, adjacent to a coenzyme A binding site. Desuccinylated by SIRT5 (By similarity).

Subcellular Location:

Mitochondrion matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in liver, kidney, pancreas, heart and skeletal muscle.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the 3-hydroxyacyl-CoA dehydrogenase family.

Research Fields

· Metabolism > Lipid metabolism > Fatty acid elongation.

· Metabolism > Lipid metabolism > Fatty acid degradation.

· Metabolism > Amino acid metabolism > Valine, leucine and isoleucine degradation.

· Metabolism > Amino acid metabolism > Lysine degradation.

· Metabolism > Amino acid metabolism > Tryptophan metabolism.

· Metabolism > Carbohydrate metabolism > Butanoate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Fatty acid metabolism.

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