GPX5 Antibody - #DF6220

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Product: GPX5 Antibody
Catalog: DF6220
Description: Rabbit polyclonal antibody to GPX5
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 25kDa; 25kD(Calculated).
Uniprot: O75715
RRID: AB_2838186

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(82%), Bovine(90%), Horse(90%), Sheep(90%), Rabbit(90%), Dog(80%)
Clonality:
Polyclonal
Specificity:
GPX5 Antibody detects endogenous levels of total GPX5.
RRID:
AB_2838186
Cite Format: Affinity Biosciences Cat# DF6220, RRID:AB_2838186.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Arep; arMEP24; EGLP; Epididymal androgen related protein; Epididymal secretory glutathione peroxidase; epididymis secretory sperm binding protein Li 75p; Epididymis specific glutathione peroxidase like protein; Epididymis-specific glutathione peroxidase-like protein; Glutathione peroxidase 5 (epididymal androgen related protein); Glutathione peroxidase 5; Glutathione peroxidase, epididymal; GPx-5; GPX5; GPX5_HUMAN; GSHPx-5; HEL-S-75p; Major androgen regulated protein; OTTHUMP00000017919; OTTHUMP00000214636;

Immunogens

Immunogen:
Uniprot:

O75715(GPX5_HUMAN) >>Visit HPA database.

Gene(ID):
GPX5(2880)
Expression:
O75715 GPX5_HUMAN:

Epididymis.

Description:
GPX5 belongs to the glutathione peroxidase family. GPX5 protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. Catalytic the reaction: 2 glutathione + H2O2 = glutathione disulfide + 2 H2O. GPX5 constitute a glutathionine peroxidase-like protective system against peroxide damage in sperm membrane lipids.
Sequence:
MTTQLRVVHLLPLLLACFVQTSPKQEKMKMDCHKDEKGTIYDYEAIALNKNEYVSFKQYVGKHILFVNVATYCGLTAQYPELNALQEELKPYGLVVLGFPCNQFGKQEPGDNKEILPGLKYVRPGGGFVPSFQLFEKGDVNGEKEQKVFSFLKHSCPHPSEILGTFKSISWDPVKVHDIRWNFEKFLVGPDGIPVMRWSHRATVSSVKTDILAYLKQFKTK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
90
Bovine
90
Sheep
90
Rabbit
90
Pig
82
Dog
80
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75715 As Substrate

Site PTM Type Enzyme
Y41 Phosphorylation
K167 Methylation

Research Backgrounds

Function:

Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. May constitute a glutathione peroxidase-like protective system against peroxide damage in sperm membrane lipids.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Epididymis.

Family&Domains:

Belongs to the glutathione peroxidase family.

Research Fields

· Metabolism > Metabolism of other amino acids > Glutathione metabolism.

· Metabolism > Lipid metabolism > Arachidonic acid metabolism.

· Organismal Systems > Endocrine system > Thyroid hormone synthesis.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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