ARPC1A Antibody - #DF6215

(3)  
Product: ARPC1A Antibody
Catalog: DF6215
Description: Rabbit polyclonal antibody to ARPC1A
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Rabbit, Chicken
Mol.Wt.: 42kDa; 42kD(Calculated).
Uniprot: Q92747
RRID: AB_2838181

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Rabbit(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
ARPC1A Antibody detects endogenous levels of total ARPC1A.
RRID:
AB_2838181
Cite Format: Affinity Biosciences Cat# DF6215, RRID:AB_2838181.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Actin binding protein (Schizosaccharomyces pombe sop2 like); Actin related protein 2/3 complex subunit 1A 41kDa; Actin-related protein 2/3 complex subunit 1A; ARC1A_HUMAN; Arc40; ARPC1A; Epididymis luminal protein 68; Epididymis secretory protein Li 307; HEL 68; HEL S 307; SOP2-like protein; SOP2Hs; SOP2L;

Immunogens

Immunogen:
Uniprot:

Q92747(ARC1A_HUMAN) >>Visit HPA database.

Gene(ID):
ARPC1A(10552)
Description:
This gene encodes one of seven subunits of the human Arp2/3 protein complex. This subunit is a member of the SOP2 family of proteins and is most similar to the protein encoded by gene ARPC1B. The similarity between these two proteins suggests that they both may function as p41 subunit of the human Arp2/3 complex that has been implicated in the control of actin polymerization in cells. It is possible that the p41 subunit is involved in assembling and maintaining the structure of the Arp2/3 complex. Multiple versions of the p41 subunit may adapt the functions of the complex to different cell types or developmental stages. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]
Sequence:
MSLHQFLLEPITCHAWNRDRTQIALSPNNHEVHIYKKNGSQWVKAHELKEHNGHITGIDWAPKSDRIVTCGADRNAYVWSQKDGVWKPTLVILRINRAATFVKWSPLENKFAVGSGARLISVCYFESENDWWVSKHIKKPIRSTVLSLDWHPNNVLLAAGSCDFKCRVFSAYIKEVDEKPASTPWGSKMPFGQLMSEFGGSGTGGWVHGVSFSASGSRLAWVSHDSTVSVADASKSVQVSTLKTEFLPLLSVSFVSENSVVAAGHDCCPMLFNYDDRGCLTFVSKLDIPKQSIQRNMSAMERFRNMDKRATTEDRNTALETLHQNSITQVSIYEVDKQDCRKFCTTGIDGAMTIWDFKTLESSIQGLRIM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Zebrafish
100
Chicken
100
Rabbit
100
Sheep
0
Dog
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92747 As Substrate

Site PTM Type Enzyme
S2 Phosphorylation
S26 Phosphorylation
K44 Ubiquitination
K49 Ubiquitination
K63 Ubiquitination
R74 Methylation
K87 Ubiquitination
K103 Ubiquitination
K110 Ubiquitination
K138 Ubiquitination
K174 Acetylation
K179 Acetylation
K179 Ubiquitination
K235 Ubiquitination
S236 Phosphorylation
K290 Ubiquitination
S298 Phosphorylation
K358 Ubiquitination

Research Backgrounds

Function:

Probably functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.

Subcellular Location:

Cytoplasm>Cytoskeleton. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Probable component of the Arp2/3 complex in which it may replace ARPC1B (Probable). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (By similarity).

Family&Domains:

Belongs to the WD repeat ARPC1 family.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

· Human Diseases > Infectious diseases: Bacterial > Shigellosis.

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

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